Results 1 to 10 of about 99,473 (144)
Two-dimensional gel electrophoresis image analysis of two Pseudomonas aeruginosa clones
Tecnología en Marcha, 2022 A classical strategy to analyse the protein content of a biological sample is the two-dimensional gel electrophoresis (2D-GE). This technique separates proteins by both isoelectric point and molecular weight, and images are taken for subsequent analyses.
José Arturo Molina-Mora +3 more
doaj +1 more source
What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?
Proteomes, 2020 Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past.
Katrin Marcus +2 more
doaj +1 more source
A Novel Spot-Enhancement Anisotropic Diffusion Method for the Improvement of Segmentation in Two-dimensional Gel Electrophoresis Images, Based on the Watershed Transform Algorithm [PDF]
Iranian Journal of Medical Physics, 2015 Introduction Two-dimensional gel electrophoresis (2DGE) is a powerful technique in proteomics for protein separation. In this technique, spot segmentation is an essential stage, which can be challenging due to problems such as overlapping spots, streaks,
Sina Shamekhi +3 more
doaj +1 more source
A semi-virtual two dimensional gel electrophoresis: IF–ESI LC-MS/MS
MethodsX, 2017 A method for increasing the productivity of ESI LC-MS/MS (electrospray ionization-liquid chromatography-tandem mass spectrometry) was proposed and applied. After IF (isoelectric focusing) of the sample using IPG (immobilized pH gradient) strip, the strip
Stanislav Naryzhny +4 more
doaj +1 more source
Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis
Proteomes, 2016 The pioneering work by Patrick H. O’Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry 1975, 250, 4007–4021).
Sandra Murphy +2 more
doaj +1 more source
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives
BioTechniques, 2008 The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms.
Haleem J. Issaq, Timothy D. Veenstra
doaj +1 more source
Establishment of two-dimensional gel electrophoresis for soybean protein isolate and its application
Grain & Oil Science and Technology, 2020 To optimize the conditions for the establishment of two-dimensional gel electrophoresis (2-DE) of soybean protein isolate (SPI), we investigated Ampholine mixture, anodic and cathodic electrolytes, loading amount of sample, acrylamide concentration, pH ...
Xinkai Lu +8 more
doaj +1 more source
Comparative Two-Dimensional Fluorescence Gel Electrophoresis [PDF]
, 2017 Abstract Comparative two-dimensional gel electrophoresis (CoFGE) is a special version of two-dimensional polyacrylamide GE (2D-PAGE) and related to difference GE (2D-DIGE). It provides reproducibility and standardisation for 2D-PAGE by introducing a reference to the experiment.
Simone König, Doreen Ackermann
openaire +4 more sources
Biomédica: revista del Instituto Nacional de Salud, 2003 En este estudio demostramos el potencial de la electroforesis en dos dimensiones (2DE) como herramienta para la caracterización del proteoma de Leishmania (expresión proteica complementaria del genoma).
Rafael Góngora +5 more
doaj +1 more source
Journal of Lipid Research, 1988 The protein heterogeneity of fractions isolated by immunoaffinity chromatography on anti-apolipoprotein A-I and anti-apolipoprotein A-II affinity columns was analyzed by high resolution two-dimensional gel electrophoresis.
R W James +8 more
doaj +1 more source