Results 41 to 50 of about 99,492 (163)

افزایش پوشش پروتئوم سلول‌های Hs578T، متعلق به سرطان پستان، در نمایه‌ی الکتروفورز دو بعدی با حذف کارآمد مداخله‌گرهای غیر پروتئینی

open access: yesمجله دانشکده پزشکی اصفهان, 2018
مقدمه: با وجود استفاده‌ی گسترده، پوشش پروتئینی پایین و الگوهای اسمیری، از مهم‌ترین موانع بازدارنده برای استفاده از مطالعات پروتئومیک مبتنی بر ژل در بررسی‌های بالینی می‌باشد.
Hamidreza Abbasi   +4 more
doaj   +1 more source

Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry

open access: yesBiological Procedures Online, 2010
The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass ...
Honoré Bent   +4 more
doaj  

Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori.

open access: yesPLoS ONE, 2013
Malpighian tubules (MTs) are highly specific organs of arthropods (Insecta, Myriapoda and Arachnida) for excretion and osmoregulation. In order to highlight the important genes and pathways involved in multi-functions of MTs, we performed a systematic ...
Xiao-wu Zhong   +7 more
doaj   +1 more source

Mass Spectrometry-based Proteomics in Acute Respiratory Distress Syndrome: A Powerful Modality for Pulmonary Precision Medicine

open access: yesChinese Medical Journal, 2016
Objective: Acute respiratory distress syndrome (ARDS) is an acute and lethal clinical syndrome that is characterized by hypoxemic respiratory failure and diffuse alveolar inflammatory damage. This review aimed to search and discuss the mass spectrometry (
Xue-Feng Xu   +4 more
doaj   +1 more source

Decreased GRP78 Protein Expression is a Potential Prognostic Marker of Oral Squamous Cell Carcinoma in Taiwan

open access: yesJournal of the Formosan Medical Association, 2010
Oral squamous cell carcinoma (OSCC) is an aggressive tumor and its occurrence in Taiwan is closely related to chronic smoking, alcohol consumption, and especially to betel quid chewing. It became the fourth most common malignant tumor of Taiwanese men in
Tze-Ta Huang   +8 more
doaj   +1 more source

Comparative phosphoproteomic analysis of blast resistant and susceptible rice cultivars in response to salicylic acid

open access: yesBMC Plant Biology, 2019
Background Salicylic acid (SA) is a significant signaling molecule that induces rice resistance against pathogen invasion. Protein phosphorylation carries out an important regulatory function in plant defense responses, while the global phosphoproteome ...
Ranran Sun   +6 more
doaj   +1 more source

Yeast DNA Replication 2D Gel Protocol

open access: yesBio-Protocol, 2012
Two-dimensional agarose gel electrophoresis (2D gel) analysis is used extensively as a method to detect origins of replication. Here, I present a simplified method for the isolation of yeast genomic DNA for 2D gel analysis from a small number of yeast ...
Yanfei Zou
doaj   +1 more source

Comparative Proteomics and Difference Gel Electrophoresis

open access: yesBioTechniques, 2007
The goal of comparative proteomics is to analyze proteome changes in response to development, disease, or environment. This is a two-step process in which proteins within cellular extracts are first fractionated to reduce sample complexity, and then the ...
Jonathan Minden
doaj   +1 more source

Study of Puccinia striiformis -related proteins in wheat using two-dimensional gel electrophoresis

open access: yesJournal of Crop Protection, 2020
Wheat is one of the strategic products and has rich nutritional value. Plant diseases are major limiting factors that reduce the yield and quality of wheat, and yellow rust Puccinia striiformis f. sp.
Sodabeh Jahanbakhsh Godehkahriz   +2 more
doaj  

Proteomics: From Protein Identification to Biological function

open access: yesCHIMIA, 2001
The term 'proteome' describes the expressed protein complement of a genome. The largely invariant genome of an individual or organism determines its potential for gene- and protein expression but does not specify which proteins are expressed in ...
Jan van Oostrum   +7 more
doaj  

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