Results 21 to 30 of about 10,232 (247)

Immunogenicity of Endolysin PlyC

Antibiotics, 2022
Endolysins are bacteriolytic enzymes derived from bacteriophages. They represent an alternative to antibiotics, since they are not susceptible to conventional antimicrobial resistance mechanisms. Since non-human proteins are efficient inducers of specific immune responses, including the IgG response or the development of an allergic response mediated ...
Marek Adam Harhala, Katarzyna Gembara, Daniel C. Nelson, Paulina Miernikiewicz, Krystyna Dąbrowska   +4 more
openaire   +3 more sources

Endolysins of bacteriophages

Journal of microbiology, epidemiology and immunobiology, 2023
Bacteriophage endolysins are a biologically active substances that play a specific role in the release of phage progeny by degrading the peptidoglycan of the host bacterium. In the light of antibiotic resistance, endolysins are considered as alternative therapeutic agents because of their exceptional ability to target bacterial cells.
Irina A. Barkova, Margarita P. Izhberdeeva, Anastasiya A. Sautkina   +2 more
openaire   +2 more sources

Crystallographic Structure Determination of Bacteriophage Endolysins [PDF]

Current Issues in Molecular Biology, 2020
Bacteriophages produce endolysins that target and cleave the hosts peptidoglycan to release their progeny at the end of the infection cycle. These proteins can be used for the eradication of pathogenic bacteria, but also for their detection. Endolysins may contain a single catalytic domain or several domains, including a cell wall binding domain.
Sanz-Gaitero, Marta, Raaij, Mark J. van
openaire   +3 more sources

Biochemical Characterization and Validation of a Catalytic Site of a Highly Thermostable Ts2631 Endolysin from the Thermus scotoductus Phage vB_Tsc2631. [PDF]

PLoS ONE, 2015
Phage vB_Tsc2631 infects the extremophilic bacterium Thermus scotoductus MAT2631 and uses the Ts2631 endolysin for the release of its progeny. The Ts2631 endolysin is the first endolysin from thermophilic bacteriophage with an experimentally validated ...
Magdalena Plotka, Anna-Karina Kaczorowska, Agnieszka Morzywolek, Joanna Makowska, Lukasz P Kozlowski, Audur Thorisdottir, Sigurlaug Skírnisdottir, Sigridur Hjörleifsdottir, Olafur H Fridjonsson, Gudmundur O Hreggvidsson, Jakob K Kristjansson, Slawomir Dabrowski, Janusz M Bujnicki, Tadeusz Kaczorowski   +13 more
doaj   +1 more source

On the Occurrence and Multimerization of Two-Polypeptide Phage Endolysins Encoded in Single Genes

Microbiology Spectrum, 2022
Bacteriophages (phages) and other viruses are extremely efficient in packing their genetic information, with several described cases of overlapping genes encoded in different open reading frames (ORFs).
Daniela Pinto, Raquel Gonçalo, Mariana Louro, Marta Sousa Silva, Guillem Hernandez, Tiago N. Cordeiro, Carlos Cordeiro, Carlos São-José   +7 more
doaj   +1 more source

A thermostable salmonella phage endolysin, Lys68, with broad bactericidal properties against gram-negative pathogens in presence of weak acids [PDF]

, 2014
Resistance rates are increasing among several problematic Gram-negative pathogens, a fact that has encouraged the development of new antimicrobial agents.
A Wohlkonig, BW Wren, E Scallan, H Nakagawa, H Nikaido, H Oliveira, H Webb, HL Alakomi, Hugo Oliveira, I Chopra, IH van Stokkum, JA Lim, Joana Azeredo, KH Schleifer, Kornelius Zeth, L Whitmore, L Whitmore, LA Compton, Leon D. Kluskens, LH Weaver, M Mastromatteo, M Schmelcher, M Walmagh, Maarten Walmagh, Maria Teresa Neves-Petersen, MD Rolfe, R Kuroki, Rob Lavigne, S Sillankorva, SA Kim, Sanna Sillankorva, SW Provencher, T Warnecke, VA Fischetti, Viruthachalam Thiagarajan, Y Briers, Y Briers, Y Briers, Y Briers   +38 more
core   +14 more sources

Synthetic biology of modular endolysins [PDF]

Biotechnology Advances, 2018
Endolysins and their derivatives have emerged in recent years as a novel class of antibacterials, which have now entered the clinical phases. Their rapid mode-of-action and proteinaceous nature differentiates them from any other class of antibiotics. A key feature of endolysins is their modularity and the opportunities that emerge thereof to customize ...
Gerstmans, Hans, Criel, Bjorn, Briers, Yves   +2 more
openaire   +3 more sources

Effect of different cloning strategies in pET-28a on solubility and functionality of a staphylococcal phage endolysin

BioTechniques, 2020
Endolysins have been studied intensively as an alternative to antibiotics. In this study, endolysin derived from a phage which infects methicillin-resistant Staphylococcus aureus (MRSA) was cloned and expressed in Escherichia coli pET28a.
Hong Y Tham, Adelene A-L Song, Khatijah Yusoff, Geok H Tan   +3 more
doaj   +1 more source

Diversity of the lysozyme fold: structure of the catalytic domain from an unusual endolysin encoded by phage Enc34

Scientific Reports, 2022
Endolysins are bacteriophage-encoded peptidoglycan-degrading enzymes with potential applications for treatment of multidrug-resistant bacterial infections. Hafnia phage Enc34 encodes an unusual endolysin with an N-terminal enzymatically active domain and
Elina Cernooka, Janis Rumnieks, Nikita Zrelovs, Kaspars Tars, Andris Kazaks   +4 more
doaj   +1 more source

Bacteriophage Endolysins as Novel Antimicrobials [PDF]

Future Microbiology, 2012
Endolysins are enzymes used by bacteriophages at the end of their replication cycle to degrade the peptidoglycan of the bacterial host from within, resulting in cell lysis and release of progeny virions. Due to the absence of an outer membrane in the Gram-positive bacterial cell wall, endolysins can access the peptidoglycan and destroy these organisms ...
Mathias, Schmelcher, David M, Donovan, Martin J, Loessner   +2 more
openaire   +2 more sources