The teneurin C-terminal domain possesses nuclease activity and is apoptogenic. [PDF]
, 2018 Teneurins are type 2 transmembrane proteins expressed by developing neurons during periods of synaptogenesis and apoptosis. Neurons expressing teneurin-1 synapse with other teneurin-1-expressing neurons, and neurons expressing teneurin-2 synapse with ...
Chiquet-Ehrismann, Ruth +2 more
core +3 more sources
F-CphI represents a new homing endonuclease family using the Endo VII catalytic motif
Mobile DNA, 2018 Background There are six known families of homing endonucleases, LAGLIDADG, GIY-YIG, HNH, His-Cys box, PD-(D/E)-XK, and EDxHD, which are characterized by their conserved residues.
Xiaoting Fang +3 more
doaj +1 more source
DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis. [PDF]
PLoS ONE, 2013 Apoptosis and necrosis, two major forms of cell death, can be distinguished morphologically and biochemically. Internucleosomal DNA fragmentation (INDF) is a biochemical hallmark of apoptosis, and caspase-activated DNase (CAD), also known as DNA ...
Ryushin Mizuta +8 more
doaj +1 more source
Molecular variation of Trypanosoma brucei subspecies as revealed by AFLP fingerprinting [PDF]
, 2002 Genetic analysis of Trypanosoma spp. depends on the detection of variation between strains. We have used the amplified fragment length polymorphism (AFLP) technique to develop a convenient and reliable method for genetic characterization of Trypanosome ...
Agbo, E.E.C. +3 more
core +4 more sources
Two restriction endonucleases [PDF]
Biochemistry and Molecular Biology Education, 2006 AbstractTerms to be familiar with before you start to solve the test: restriction endonucleases; palindromic sequences; blunt ends; cohesive (“sticky”) ends; in vitro DNA synthesis; radioactive nucleotide precursors; template‐primer complexes; DNA ligation.
openaire +3 more sources
The pinewood nematode, Bursaphelenchus xylophilus, in Madeira Island [PDF]
, 2012 The environmental conditions in Madeira Island are favorable for the presence and dissemination of the pinewood nematode (PWN), Bursaphelenchus xylophilus.
Abrantes, Isabel +7 more
core +1 more source
SLX4 Assembles a Telomere Maintenance Toolkit by Bridging Multiple Endonucleases with Telomeres
Cell Reports, 2013 SLX4 interacts with several endonucleases to resolve structural barriers in DNA metabolism. SLX4 also interacts with telomeric protein TRF2 in human cells. The molecular mechanism of these interactions at telomeres remains unknown.
Bingbing Wan +13 more
doaj +1 more source
Machines on Genes: Enzymes that Make, Break and Move DNA and RNA [PDF]
, 2010 As the vital information repositories of the cell, the nucleic acids DNA and RNA pose many challenges as enzyme substrates. To produce, maintain and repair DNA and RNA, and to extract the genetic information that they encode, a battery of remarkable ...
Bates +13 more
core +1 more source
Chimeric restriction endonuclease. [PDF]
Proceedings of the National Academy of Sciences, 1994 Fok I restriction endonuclease recognizes the nonpalindromic pentadeoxyribonucleotide 5'-GGATG-3'.5'-CATCC-3' in duplex DNA and cleaves 9 and 13 nt away from the recognition site. Recently, we reported the presence of two distinct and separable domains within this enzyme: one for the sequence-specific recognition of DNA (the DNA-binding domain) and the
Yang Gyun Kim, Srinivasan Chandrasegaran
openaire +3 more sources
Plasmid-based lacZalpha assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase [PDF]
, 2009 The preparation of a gapped pUC18 derivative, containing the lacZalpha reporter gene in the single-stranded region, is described. Gapping is achieved by flanking the lacZalpha gene with sites for two related nicking endonucleases, enabling the excision ...
Bebenek +28 more
core +2 more sources