Results 41 to 50 of about 155,310 (382)
Journal of Biological Chemistry, 2018 Viruses rely on many host cell processes, including the cellular transcription machinery. Segmented negative-strand RNA viruses (sNSV) in particular cannot synthesize the 5′-cap structure for their mRNA but cleave off cellular caps and use the resulting ...
Tobias Holm +7 more
semanticscholar +1 more source
Type II restriction endonucleases — a historical perspective and more
Nucleic Acids Research, 2016 This article continues the series of Surveys and Summaries on restriction endonucleases (REases) begun this year in Nucleic Acids Research. Here we discuss ‘Type II’ REases, the kind used for DNA analysis and cloning. We focus on their biochemistry: what
A. Pingoud, G. Wilson, W. Wende
semanticscholar +1 more source
Competitive Activation of a Methyl C−H Bond of Dimethylformamide at an Iridium Center [PDF]
, 2009 During the synthesis of [AsPh_4][Ir(CO)_2I_3Me] by refluxing IrCl_3·3H_2O in DMF (DMF = dimethylformamide) in the presence of aqueous HCl, followed by sequential treatment with [AsPh_4]Cl, NaI, and methyl iodide and finally recrystallization from ...
Bercaw, John E. +4 more
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Generation of genetically-engineered animals using engineered endonucleases
Archives of pharmacal research, 2018 The key to successful drug discovery and development is to find the most suitable animal model of human diseases for the preclinical studies. The recent emergence of engineered endonucleases is allowing for efficient and precise genome editing, which can
Jong Geol Lee, Y. Sung, In-Jeoung Baek
semanticscholar +1 more source
Genes and Environment, 2017 Apurinic/apyrimidinic (AP) sites are one of the most frequent DNA lesions. AP sites inhibit transcription and DNA replication, and induce cell death. AP endonucleases are key enzymes in AP site repair. Several types of AP endonucleases have been reported,
Masafumi Funakoshi +3 more
doaj +1 more source
Frontiers in Microbiology, 2022 Strand-specific DNA nicking endonucleases (NEases) typically nick 3–7 bp sites. Our goal is to engineer infrequent NEase with a >8 bp recognition sequence.
Shuang-yong Xu
doaj +1 more source
Modular Nucleic Acid Assembled p/MHC Microarrays for Multiplexed Sorting of Antigen-Specific T Cells [PDF]
, 2009 The human immune system consists of a large number of T cells capable of recognizing and responding to antigens derived from various sources. The development of peptide-major histocompatibility (p/MHC) tetrameric complexes has enabled the direct ...
Bailey, Ryan C. +12 more
core +2 more sources
In vivo cleavage specificity of Trypanosoma brucei editosome endonucleases
Nucleic Acids Research, 2017 RNA editing is an essential post-transcriptional process that creates functional mitochondrial mRNAs in Kinetoplastids. Multiprotein editosomes catalyze pre-mRNA cleavage, uridine (U) insertion or deletion, and ligation as specified by guide RNAs.
J. Carnes +5 more
semanticscholar +1 more source
Two restriction endonucleases [PDF]
Biochemistry and Molecular Biology Education, 2006 AbstractTerms to be familiar with before you start to solve the test: restriction endonucleases; palindromic sequences; blunt ends; cohesive (“sticky”) ends; in vitro DNA synthesis; radioactive nucleotide precursors; template‐primer complexes; DNA ligation.
openaire +2 more sources
Rapid identification and differentiation of the vaccine strain Rac H from EHV 1 field isolates using a non-radioactive DNA probe [PDF]
, 1992 A method for rapid differentiation between the EHV 1 live vaccine strain Rac H and field isolates is described. Total DNA was isolated from virus-infected small scale cell cultures.
Eichhorn, Werner +3 more
core +1 more source