HOPS-Dependent Endosomal Escape Demands Protein Unfolding [PDF]
ACS Central ScienceThe inefficient translocation of proteins across biological membranes limits their application as potential therapeutics and research tools. In many cases, the translocation of a protein involves two discrete steps: uptake into the endocytic pathway and endosomal escape.
Madeline Zoltek +5 more
doaj +6 more sources
Vapor nanobubble is the more reliable photothermal mechanism for inducing endosomal escape of siRNA without disturbing cell homeostasis [PDF]
Journal of Controlled Release, 2020 Strategies for controlled delivery of therapeutic siRNA into living cells are in high demand as endosomal escape remains the most prominent bottleneck at the intracellular level.
Juan C Fraire +2 more
exaly +4 more sources
Stochastic Model of Virus–Endosome Fusion and Endosomal Escape of pH-Responsive Nanoparticles [PDF]
Mathematics, 2022 In this paper, we set up a stochastic model for the dynamics of active Rab5 and Rab7 proteins on the surface of endosomes and the acidification process that govern the virus–endosome fusion and endosomal escape of pH-responsive nanoparticles. We employ a
Sergei Fedotov +3 more
doaj +4 more sources
The endosomal escape vehicle platform enhances delivery of oligonucleotides in preclinical models of neuromuscular disorders [PDF]
Molecular Therapy: Nucleic Acids, 2023 Biological therapeutic agents are highly targeted and potent but limited in their ability to reach intracellular targets. These limitations often necessitate high therapeutic doses and can be associated with less-than-optimal therapeutic activity.
Xiang Li +15 more
doaj +2 more sources
Modular Calcium‐Responsive and CD9‐Targeted Phospholipase System Enhancing Endosomal Escape for DNA Delivery [PDF]
Advanced ScienceGene delivery systems must overcome multiple barriers, with endosomal escape representing a prominent obstacle. We have previously shown that a bacterial phospholipase C (PLC) enabled endosomal escape of a non‐viral protein‐based DNA delivery system ...
Alexander Klipp +5 more
doaj +2 more sources
Mechanistic insights into endosomal escape by sodium oleate-modified liposomes [PDF]
Beilstein Journal of NanotechnologyEndosomal entrapment significantly limits the efficacy of drug delivery systems. This study investigates sodium oleate-modified liposomes (SO-Lipo) as an innovative strategy to enhance endosomal escape and improve cytosolic delivery in 4T1 triple ...
Ebrahim Sadaqa +3 more
doaj +2 more sources
Endosomal Escape and Nuclear Localization: Critical Barriers for Therapeutic Nucleic Acids [PDF]
MoleculesTherapeutic nucleic acids (TNAs) including antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) have emerged as promising treatment strategies for a wide variety of diseases, offering the potential to modulate gene expression with a high ...
Randall Allen, Toshifumi Yokota
doaj +2 more sources
Endosomal Escape of Lipid Nanoparticles: A Perspective on the Literature Data. [PDF]
ACS NanoEndosomal escape remains a critical bottleneck for the intracellular delivery of nucleic acids by lipid nanoparticles (LNPs), largely due to its low efficiency and poorly understood mechanism. While various models, including proton sponge effect/osmotic lysis and membrane destabilization/fusion, have been proposed, none are fully validated or ...
Pei D.
europepmc +3 more sources
Globoside Is an Essential Intracellular Factor Required for Parvovirus B19 Endosomal Escape [PDF]
CellsHuman parvovirus B19 (B19V), like most parvoviruses, possesses phospholipase A2 (PLA2) activity, which is thought to mediate endosomal escape by membrane disruption.
Jan Bieri +5 more
doaj +2 more sources
Sodium oleate functionalized simvastatin liposomes: boosting endosomal escape and anticancer efficacy in triple negative breast cancer [PDF]
Research in Pharmaceutical SciencesBackground and purpose: Due to delivery obstacles, Simvastatin, a potential anticancer agent, faces clinical limitations. This study aimed to enhance simvastatin delivery and efficacy against triple-negative breast cancer (TNBC) by developing liposomes ...
Ebrahim Sadaqa +3 more
doaj +2 more sources