Endosomal escape: A bottleneck for LNP-mediated therapeutics. [PDF]
Proc Natl Acad Sci U S ALipid nanoparticles (LNPs) have recently emerged as a powerful and versatile clinically approved platform for nucleic acid delivery, specifically for mRNA vaccines. A major bottleneck in the field is the release of mRNA-LNPs from the endosomal pathways into the cytosol of cells where they can execute their encoded functions.
Chatterjee S, Kon E, Sharma P, Peer D.
europepmc +4 more sources
Limiting endosomal damage sensing reduces inflammation triggered by lipid nanoparticle endosomal escape. [PDF]
Nat NanotechnolLipid nanoparticles (LNPs) have emerged as the dominant platform for RNA delivery, but they induce severe inflammation. Here we show that LNPs' hallmark feature, endosomal escape, which is necessary for RNA expression, also triggers inflammation by causing endosomal membrane damage.
Omo-Lamai S +38 more
europepmc +4 more sources
Chloroquine and cytosolic galectins affect endosomal escape of antisense oligonucleotides after Stabilin-mediated endocytosis [PDF]
Molecular Therapy: Nucleic Acids, 2023 Non-DNA-binding Stabilin-2/HARE receptors expressed on liver sinusoidal endothelial cells specifically bind to and internalize several classes of phosphorothioate antisense oligonucleotides (PS-ASOs).
Ekta Pandey, Edward N. Harris
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Thiol‐Disulfide Exchange as a Route for Endosomal Escape of Polymeric Nanoparticles [PDF]
Angewandte Chemie - International Edition, 2022 Endosomal entrapment has remained the major bottleneck for cytosolic delivery of nanoparticle-based delivery systems. Uncovering fundamentally new pathways for endosomal escape is therefore highly sought.
Pintu Kanjilal +2 more
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Imaging small molecule-induced endosomal escape of siRNA [PDF]
Nature Communications, 2020 Therapeutic siRNA becomes trapped in endosomes, limiting its efficacy. Here the authors use fluorescently-tagged galectin-9 as a biosensor for membrane damage to monitor endosomal escape of cholesterol-conjugated siRNA following treatment of small ...
Hampus Du Rietz +4 more
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The Biology of Endosomal Escape: Strategies for Enhanced Delivery of Therapeutics. [PDF]
ACS NanoIntracellular delivery of biomolecules is essential to the success of modern therapeutics, yet endosomal entrapment remains a critical barrier to efficacy, and this has been highlighted with the push toward lipid nanoparticle (LNP) therapeutic delivery.
Chahal GS +3 more
europepmc +6 more sources
Quantitating Endosomal Escape of a Library of Polymers for mRNA Delivery
Nano Letters, 2020 Endosomal escape is a key step for intracellular drug delivery of nucleic acids, but reliable and sensitive methods for its quantitation remain an unmet need. In order to rationally optimize the mRNA transfection efficiency of a library of polymeric materials, we designed a deactivated Renilla luciferase-derived molecular probe whose activity can be ...
Yuhang Jiang, Qiao Lu, Yongheng Wang
exaly +4 more sources
Pharmaceuticals, 2012 Cell penetrating peptides (CPPs) can deliver cell-impermeable therapeutic cargos into cells. In particular, CPP-cargo conjugates tend to accumulate inside cells by endocytosis.
Alfredo Erazo-Oliveras +2 more
exaly +3 more sources
Assessing cellular internalization and endosomal escape abilities of novel BUFII-Graphene oxide nanobioconjugates [PDF]
Frontiers in Chemistry, 2022 Cell-penetrating agents based on functionalized nanoplatforms have emerged as a promising approach for developing more efficient and multifunctional delivery vehicles for treating various complex diseases that require reaching different intracellular ...
Julian Daniel Torres-Vanegas +6 more
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Enhancing endosomal escape of transduced proteins by photochemical internalisation. [PDF]
PLoS ONE, 2012 Induced internalisation of functional proteins into cultured cells has become an important aspect in a rising number of in vitro and in vivo assays. The endo-lysosomal entrapment of the transduced proteins remains the major problem in all transduction ...
Kevin Mellert +4 more
doaj +4 more sources