DNA-inspired nanomaterials for enhanced endosomal escape [PDF]
Proceedings of the National Academy of Sciences, 2021 To realize RNA interference (RNAi) therapeutics, it is necessary to deliver therapeutic RNAs (such as small interfering RNA or siRNA) into cell cytoplasm. A major challenge of RNAi therapeutics is the endosomal entrapment of the delivered siRNA. In this study, we developed a family of delivery vehicles called Janus base nanopieces (NPs).
Jinhyung Lee +4 more
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Engineered lipid nanoparticles with synergistic dendritic cell targeting and enhanced endosomal escape for boosted mRNA cancer vaccines. [PDF]
Mater Today BioLipid nanoparticles (LNPs) have emerged as a pivotal carriers for enhancing mRNA therapeutics, particularly in antitumor therapy. However, achieving robust antigen expression remains a major challenge due to limitations in precise targeted delivery and ...
Wang S +8 more
europepmc +2 more sources
Breaking free: endocytosis and endosomal escape of extracellular vesicles. [PDF]
Extracell Vesicles Circ Nucl Acids, 2023 Extracellular vesicles (EVs) are natural micro-/nanoparticles that play an important role in intercellular communication. They are secreted by producer/donor cells and subsequent uptake by recipient/acceptor cells may result in phenotypic changes in ...
Ribovski L, Joshi B, Gao J, Zuhorn I.
europepmc +2 more sources
pH-Responsive Amphiphilic Carboxylate Polymers: Design and Potential for Endosomal Escape
Frontiers in Chemistry, 2021 The intracellular delivery of emerging biomacromolecular therapeutics, such as genes, peptides, and proteins, remains a great challenge. Unlike small hydrophobic drugs, these biotherapeutics are impermeable to the cell membrane, thus relying on the ...
Shiqi Wang
doaj +1 more source
Communications Biology, 2021 Munson et al. develop a high-throughput Galectin-9 reporter based endosomal escape imaging assay to probe correlations between nanoparticle-mediated uptake, endosomal escape frequency, and mRNA translation.
Michael J. Munson +8 more
doaj +1 more source
Endosomal escape of RNA therapeutics: How do we solve this rate-limiting problem? [PDF]
RNA, 2023 With over 15 FDA approved drugs on the market and numerous ongoing clinical trials, RNA therapeutics, such as small interfering RNAs (siRNAs) and antisense oligonucleotides (ASOs), have shown great potential to treat human disease.
Dowdy SF.
europepmc +2 more sources
Engineered ionizable lipid siRNA conjugates enhance endosomal escape but induce toxicity in vivo [PDF]
Journal of Controlled Release, 2022 Annabelle Biscans +2 more
exaly +2 more sources
Endosomal escape mechanisms of extracellular vesicle-based drug carriers: lessons for lipid nanoparticle design. [PDF]
Extracell Vesicles Circ Nucl AcidsThe rise of biologics and RNA-based therapies challenges the limitations of traditional drug treatments. However, these potent new classes of therapeutics require effective delivery systems to reach their full potential.
Hagedorn L +3 more
europepmc +2 more sources
Molecules, 2020 Endosomal escape in cell-penetrating peptide (CPP)-based drug/macromolecule delivery systems is frequently insufficient. The CPP-fused molecules tend to remain trapped inside endosomes and end up being degraded rather than delivered into the cytosol. One
Tet Htut Soe +2 more
doaj +1 more source
Endosomal escape of delivered mRNA from endosomal recycling tubules visualized at the nanoscale [PDF]
Journal of Cell Biology, 2020 Abstract Delivery of exogenous mRNA using lipid nanoparticles (LNP) is a promising strategy for therapeutics. However, a bottleneck remains the poor understanding of the parameters that correlate with endosomal escape vs. cytotoxicity.
Prasath Paramasivam +13 more
openaire +4 more sources