Metal–Phenolic Coatings as a Platform to Trigger Endosomal Escape of Nanoparticles [PDF]
ACS Nano, 2019 The intracellular delivery of functional nanoparticles (NPs) and the release of therapeutic payloads at a target site are central issues for biomedical applications. However, the endosomal entrapment of NPs typically results in the degradation of active cargo, leading to poor therapeutic outcomes. Current advances to promote the endosomal escape of NPs
Jingqu Chen, Jianhua Li, Jiajing Zhou
exaly +4 more sources
Endosomal escape in magnetic nanostructures: Recent advances and future perspectives
Materials Today AdvancesSeveral evolving therapies depend on the delivery of therapeutic cargo into the cytoplasm. Engineered magnetic nanoparticles (MNPs) have played a pivotal role in advancing and modernizing cancer theranostics, vaccination and gene therapies.
Shubhangi D. Shirsat +8 more
doaj +2 more sources
Delivery of RNA Therapeutics: The Great Endosomal Escape! [PDF]
Nucleic Acid Ther, 2022 RNA therapeutics, including siRNAs, antisense oligonucleotides, and other oligonucleotides, have great potential to selectively treat a multitude of human diseases, from cancer to COVID to Parkinson's disease. RNA therapeutic activity is mechanistically driven by Watson–Crick base pairing to the target gene RNA without the requirement of prior ...
Dowdy SF, Setten RL, Cui XS, Jadhav SG.
europepmc +3 more sources
Strategies and mechanisms for endosomal escape of therapeutic nucleic acids
Current Opinion in Chemical BiologyDespite impressive recent establishment of therapeutic nucleic acids as drugs and vaccines, their broader medical use is impaired by modest performance in intracellular delivery. Inefficient endosomal escape presents a major limitation responsible for inadequate cytosolic cargo release.
Melina Grau, Ernst Wagner
openaire +4 more sources
Development of a Cationic Polymeric Micellar Structure with Endosomal Escape Capability Enables Enhanced Intramuscular Transfection of mRNA-LNPs [PDF]
VaccinesBackground/Objectives: The endosomal escape of lipid nanoparticles (LNPs) is crucial for efficient mRNA-based therapeutics. Here, we present a cationic polymeric micelle (cPM) as a safe and potent co-delivery system with enhanced endosomal escape ...
Siyuan Deng +7 more
doaj +2 more sources
A cleavable peptide adapter augments the activity of targeted toxins in combination with the glycosidic endosomal escape enhancer SO1861 [PDF]
BMC BiotechnologyBackground Treatment with tumor-targeted toxins attempts to overcome the disadvantages of conventional cancer therapies by directing a drug’s cytotoxic effect specifically towards cancer cells.
Finn J. Schulze +5 more
doaj +2 more sources
PLoS ONE, 2017 A recent theory suggests that endocytosis is involved in uptake and intracellular transport of electrotransfected plasmid DNA (pDNA). The goal of the current study was to understand if approaches used previously to improve endocytosis of gene delivery ...
Lisa D Cervia +3 more
doaj +3 more sources
Nanoscopy for endosomal escape quantification
Nanoscale Advances, 2021 How nanoscopy can be applied towards the study and quantification of endosomal escape of nanoparticles.
Teodora Andrian +3 more
openaire +5 more sources
Unravelling cytosolic delivery of endosomal escape peptides with a quantitative endosomal escape assay (SLEEQ) [PDF]
ACS Applied Materials & Interfaces, 2020 Abstract Endosomal escape is an essential requirement but a major obstacle to efficient delivery of therapeutic peptides, proteins and nucleic acids. Current understanding of endosomal escape mechanisms remains limited due to significant number of conflicting reports, which are compounded by low sensitivity and indirect assays.
Serena L.Y. Teo +5 more
openaire +3 more sources
Mechanism of pH-sensitive Amphiphilic Endosomal Escape of Ionizable Lipid Nanoparticles for Cytosolic Nucleic Acid Delivery. [PDF]
Pharm ResLipid nanoparticles (LNPs) are among the most successful classes of nonviral delivery systems for nucleic acid-based therapeutics in treating human diseases.
Lu ZR, Sun D.
europepmc +2 more sources