Results 81 to 90 of about 47,261 (313)

Polycation gene delivery systems: escape from endosomes to cytosol [PDF]

Journal of Pharmacy and Pharmacology, 2003
Abstract Clinical success of gene therapy based on oligonucleotides (ODNs), ribozymes, RNA and DNA will be greatly dependent on the availability of effective delivery systems. Polycations have gained increasing attention as a non-viral gene delivery vector in the past decades.
Cho, YW, Kim, JD Kim, Jong-Duk, Park, K
openaire   +2 more sources

Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay

Nature Communications, 2021
Cytosolic transport is an essential requirement but a major obstacle to efficient delivery of therapeutic peptides, proteins and nucleic acids. Current understanding of cytosolic delivery mechanisms remains limited due to a significant number of ...
S. L. Teo, Joshua J. Rennick, D. Yuen, Hareth Al-Wassiti, A. Johnston, C. Pouton   +5 more
semanticscholar   +1 more source

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Mutational Analysis of RIP Type I Dianthin-30 Suggests a Role for Arg24 in Endocytosis

Toxins
Saponin-mediated endosomal escape is a mechanism that increases the cytotoxicity of type I ribosome-inactivating proteins (type I RIPs). In order to actualize their cytotoxicity, type I RIPs must be released into the cytosol after endocytosis.
Louisa Schlaak, Christoph Weise, Benno Kuropka, Alexander Weng   +3 more
doaj   +1 more source

Structural Dynamics and Activity of B19V VP1u during the pHs of Cell Entry and Endosomal Trafficking

Viruses, 2022
Parvovirus B19 (B19V) is a human pathogen that is the causative agent of fifth disease in children. It is also known to cause hydrops in fetuses, anemia in AIDS patients, and transient aplastic crisis in patients with sickle cell disease.
Renuk V. Lakshmanan, Joshua A. Hull, Luke Berry, Matthew Burg, Brian Bothner, Robert McKenna, Mavis Agbandje-McKenna   +6 more
doaj   +1 more source

Transferrinfection. A Highly Efficient Way to Express Gene Constructs in Eukaryotic Cells [PDF]

, 1992
No abstract ...
Atherton, Atherton, Chardonnet, CHRISTIAN PLANK, Cotten, Cotten, Cotten, Curiel, Curiel, DAVID T. CURIEL, Davidson, Day, ERNST WAGNER, Folk, Jones, Krol, KURT ZATLOUKAL, Lim, MATT COTTEN, MAX L. BIRNSTIEL, Mol, Pastan, PETER STEINLEIN, Powell, Sambrook, Sipe, Spiess, STEPHEN PHILLIPS, Stout, Stryer, Wagner, Wagner, Wharton, Wu, Wu, Zenke   +35 more
core   +1 more source

Fluorescence-based quantification of messenger RNA and plasmid DNA decay kinetics in extracellular biological fluids and cell extracts [PDF]

, 2020
Extracellular and intracellular degradation of nucleic acids remains an issue in non-viral gene therapy. Understanding biodegradation is critical for the rational design of gene therapeutics in order to maintain stability and functionality at the target ...
Braeckmans, Kevin, De Smedt, Stefaan, Raes, Laurens, Remaut, Katrien, Rombouts, Koen, Xiong, Ranhua, Zhang, Heyang   +6 more
core   +1 more source

Smart, Bio‐Inspired Polymers and Bio‐Based Molecules Modified by Zwitterionic Motifs to Design Next‐Generation Materials for Medical Applications

Advanced Functional Materials, EarlyView.
Bio‐based and (semi‐)synthetic zwitterion‐modified novel materials and fully synthetic next‐generation alternatives show the importance of material design for different biomedical applications. The zwitterionic character affects the physiochemical behavior of the material and deepens the understanding of chemical interaction mechanisms within the ...
Theresa M. Lutz, Jonas De Breuck, Sahar Salehi, Meike N. Leiske   +3 more
wiley   +1 more source

The thermodynamics of endosomal escape and DNA release from lipoplexes [PDF]

Physical Chemistry Chemical Physics, 2016
In this work we identify and characterize the entropic driving forces governing the process of transfection by lipid–DNA complexes.
Avital, Yotam Y., Grønbech-Jensen, Niels, Farago, Oded   +2 more
openaire   +3 more sources

Histidine-based coordinative polymers for efficient intracellular protein delivery via enhanced protein binding, cellular uptake, and endosomal escape.

Biomaterials Science, 2023
Polymers are one of the most promising protein delivery carriers; however, their applications are hindered by low delivery efficacy owing to their undesirable performance in protein binding, cellular uptake and endosomal escape.
Chang-Xiang Chen, Peng Gao, Hui Wang, Yiyun Cheng, Jia Lv   +4 more
semanticscholar   +1 more source