Results 91 to 100 of about 107,252 (310)

Understanding and Overcoming Antibody‐Drug Conjugate Resistance: Biological Mechanisms and Emerging Analytical Frameworks in Breast Cancer

Advanced Science, EarlyView.
Antibody–drug conjugates (ADCs) transform breast cancer therapy, yet resistance limits their durability. Emerging evidence reveals that ADC failure is not solely tumor‐intrinsic but shaped by dynamic tumor–microenvironment interactions that alter drug delivery, processing, and response.
Minji Seo, Jangsoon Lee, Naoto T. Ueno
wiley   +1 more source

The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomes and late endosomes

, 2000
Endobrevin/VAMP-8 is an R-SNARE localized to endosomes, but it is unknown in which intracellular fusion step it operates. Using subcellular fractionation and quantitative immunogold electron microscopy, we found that endobrevin/VAMP-8 is present on all ...
Honing, S., Jahn, R., Tikkanen, R., Antonin, W., Holroyd, C.   +4 more
core   +1 more source

Cryo‐EM Structure Guided Engineering of Botulinum Neurotoxin A With Advanced Receptor Binding Affinity and Therapeutical Benefits

Advanced Science, EarlyView.
The butterfly unfolded wing in an open form structure of botulinum neurotoxin type A (BoNT/A) at physiological‐state was confirmed at 2.85 Å resolution by cryo‐electron microscopy (cryo‐EM). Structure‐guided protein engineering significantly enhanced the receptor‐binding affinity, therapeutic efficacy, and safety of the engineered toxin variants ...
Wenrui Wang, Zhaxi Zerang, Linjin You, Ziye Liu, Rong Nie, Fuwei Qi, Fenfen Gao, Chengmu Zhao, Wantong Ma, Jinghan He, Xiaoru Wang, Shanquan Wu, Bo Liu, Xinyao Liu, Dongsheng Lei, Dejuan Zhi, Dongsheng Wang   +16 more
wiley   +1 more source

Combating VEGFA‐siRNA‐Induced Metabolic Reprogramming via Glucose Utilization Deprivation

Advanced Science, EarlyView.
An ionizable lipid nanoparticle co‐delivers VEGFA siRNA and glucose oxidase to tumors to counteract VEGFA knockdown‐induced, glutamine‐driven metabolic compensation. Enzymatic glucose consumption reverses the adaptive pathways and restores metabolism toward baseline, thereby sensitizing tumors to therapy.
Lulu Zheng, Shuai Guo, Yingjixing Luo, Pengfei Wu, Haiyin Yang, Yingqiu Xie, Yuchuan Fan, Qing Liu, Bo Hu, Jia Huang, Yuanyu Huang   +10 more
wiley   +1 more source

Magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles

Cell Structure and Function
Membrane stiffness is essential for cell migration, tumorigenesis, and development; however, the physical properties of intracellular membrane are poorly characterized.
Yuta Yonekawa, Kazuki Oikawa, Boldbaatar Bayarkhuu, Kizuna Kobayashi, Nana Saito, Ibuki Oikawa, Ryohei Yamada, Yu-han Chen, Koichi Oyanagi, Yuji Shibasaki, Satoru Kobayashi, Yoko Shiba   +11 more
doaj   +1 more source

Autophagosome maturation in primary rat hepatocytes.

, 2006
Nutrient deprivation of eukaryotic cells elicits a rapid survival response, including the induction of autophagy. Autophagy, or "self-eating", involves the formation of autophagosomes from an unknown membrane source and the sequestration of cytosolic ...
Kchl, R., Köchl, Robert
core  

Magnetic endosomes, probes inside living cells.

, 2013
Magnetic nanoparticles (black points on the electron micrograph, top left) concentrate within intracellular endosomes about 0.6 µm in diameter. Each endosome acquires a magnetic moment when submitted to a magnetic field B, resulting in the formation of ...
Thi-Hanh Nguyen (365851), Damien Robert (365850), François Gallet (365852), Claire Wilhelm (365853)   +3 more
core   +1 more source

ESCRT‐Mimetic Nanodegrader Targets STING for Anti‐Inflammatory Therapy

Advanced Science, EarlyView.
A nanoplatform‐enabled targeted protein degradation strategy is presented to regulate aberrant STING signaling. STING‐ATTEC induces selective autophagic degradation of STING via formation of a STING–ATTEC–LC3 ternary complex, while the cationic FA‐LNP+ system enhances LC3 generation and targeted delivery. Together, this synergistic approach efficiently
Fuyuan Zhou, Qiming Zhai, Zhihao Yao, Qing Li, Yan Yang, Si Wang, Chao Huang, Liangjing Xin, Tao Chen, Jinlin Song   +9 more
wiley   +1 more source

Molecular characterization of proprotein convertase subtilisin/kexin type 9-mediated degradation of the LDLR

Journal of Lipid Research, 2012
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a secreted protein that promotes degradation of cell surface LDL receptors (LDLRs) in selected cell types.
Yan Wang, Yongcheng Huang, Helen H. Hobbs, Jonathan C. Cohen   +3 more
doaj   +1 more source

Phagophores evolve from recycling endosomes.

, 2018
The membrane origins of autophagosomes have been a key unresolved question in the field. The earliest morphologically recognizable structure in the macroautophagy/autophagy itinerary is the double-membraned cup-shaped phagophore.

core   +1 more source