Results 11 to 20 of about 2,029 (168)
Generation of equine enteroids and enteroid-derived 2D monolayers that are responsive to microbial mimics [PDF]
Veterinary Research, 2021 Enteroid cultures are three-dimensional in vitro models that reflect the cellular composition and architecture of the small intestine. One limitation with the enteroid conformation is the enclosed lumen, making it difficult to expose the apical surface ...
Stina Hellman, Hellman, Stina
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Establishment of Bovine 3D Enteroid-derived 2D monolayers [PDF]
Veterinary Research, 2022 Three-dimensional (3D) intestinal enteroids are powerful in vitro models for studying intestinal biology. However, due to their closed structure direct access to the apical surface is impeded, limiting high-throughput applications of exogenous compounds ...
Vervelde, Lonneke +4 more
core +6 more sources
, 2021 Additional file 3. Equine enteroid-derived 2D monolayers exposed to Poly I:C + Lipofectin (A) or Lipofectin alone (B) and the relative expression of cytokine genes (C) after 20 h stimulation.
Stina Hellman (11282114)
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Intestinal Enteroid Culture for Human Astroviruses [PDF]
BIO-PROTOCOL, 2020 Human astroviruses (HAstV) are non-enveloped, positive-sense single stranded RNA viruses that typically cause gastroenteritis in children, the elderly and among immunocompromised individuals. Some HAstV species have also been implicated in neurological diseases.
Irene Owusu +2 more
openaire +3 more sources
Whole-mount Enteroid Proliferation Staining [PDF]
BIO-PROTOCOL, 2016 Small intestinal organoids, otherwise known as enteroids, have become an increasingly utilized model for intestinal biology in vitro as they recapitulate the various epithelial cells within the intestinal crypt (Mahe et al., 2013; Sato et al., 2009).
Caitlyn Barrett +3 more
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Organoid and Enteroid Modeling of Salmonella Infection [PDF]
Frontiers in Cellular and Infection Microbiology, 2018 Salmonella are Gram-negative rod-shaped facultative anaerobic bacteria that are comprised of over 2,000 serovars. They cause gastroenteritis (salmonellosis) with headache, abdominal pain and diarrhea clinical symptoms. Salmonellosis brings a heavy burden for the public health in both developing and developed countries. Antibiotics are usually effective
Yuebang Yin, Daoguo Zhou, Daoguo Zhou
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Human Sapovirus Replication in Human Intestinal Enteroids
Journal of Virology, 2023 Human sapoviruses (HuSaVs) are a frequent but overlooked cause of acute gastroenteritis, especially in children. Little is known about this pathogen, whose successful in vitro cultivation was reported only recently, in a cancer cell-derived line.
Gabriel Euller-Nicolas +8 more
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3D enteroid infection by microinjection of Giardia intestinalis trophozoites expressing mNeonGreen.
, 2023 (A) G. intestinalis microinjection with a non-optimised microinjection needle (G. intestinalis microinjection needles with wider openings (7–9 μm diameter). Scale bar = 10 μm. (C) Microinjection of a 3D enteroid with an optimised G.
Jana Grüttner (12467874) +8 more
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, 2022 TEER values of differentiated ileal enteroid monolayers (35I) were recorded prior to and after 4 hr infection (A). The change in TEER over time is expressed as TEER (percentage change) and data presented are pooled from six independent experiments.
Emily M. Smith (1326564) +4 more
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From 3D Organoids back to 2D Enteroids [PDF]
Developmental Cell, 2018 In this issue of Developmental Cell, Thorne et al. (2018) describe a simple, scalable method to culture 2D enteroid monolayers that surprisingly recapitulates many of the features of 3D organoid cultures and in vivo intestinal tissue and can be used for high-throughput microscopy-based experiments.
Jonathan, Braverman, Ömer H, Yilmaz
openaire +2 more sources