Results 171 to 180 of about 3,117 (204)

Apical-Out Enteroids as an Innovative Model for Necrotizing Enterocolitis

Journal of Surgical Research, 2023
Necrotizing enterocolitis (NEC) is a gastrointestinal disease of premature neonates. We previously validated a NEC enteroid model derived from human infant intestinal tissue. Typical enteroid configuration is basolateral-out (BO) without direct access to the luminal (apical) surface.
Heather Liebe, Camille Schlegel, Alena Golubkova   +2 more
exaly   +3 more sources

Quantification of Proliferative and Dead Cells in Enteroids

Journal of Visualized Experiments, 2020
The intestinal epithelium acts as a barrier that prevents luminal contents, such as pathogenic microbiota and toxins, from entering the rest of the body. Epithelial barrier function requires the integrity of intestinal epithelial cells. While epithelial cell proliferation maintains a continuous layer of cells that forms a barrier, epithelial damage ...
Hua-Shan, Li, Shao-Fang, Xu, Jian-Ying, Sheng, Zhi-Hui, Jiang, Jing, Wang, Ning, Ding, Tao, Wang, Matthew A, Odenwald, Jerrold R, Turner, Wei-Qi, He, Hong, Xu, Juan-Min, Zha   +11 more
openaire   +2 more sources

Age-Specific ADME Gene Expression in Infant Intestinal Enteroids [PDF]

Molecular Pharmaceutics
In childhood, developmental changes and environmental interactions highly affect orally dosed drug disposition across the age range. To optimize dosing regimens and ensure safe use of drugs in pediatric patients, understanding this age-dependent biology ...
Saskia N de Wildt
exaly   +2 more sources

Effect of essential amino acids on enteroids: Methionine deprivation suppresses proliferation and affects differentiation in enteroid stem cells

Biochemical and Biophysical Research Communications, 2017
We investigated the effects of essential amino acids on intestinal stem cell proliferation and differentiation using murine small intestinal organoids (enteroids) from the jejunum. By selectively removing individual essential amino acids from culture medium, we found that 24 h of methionine (Met) deprivation markedly suppressed cell proliferation in ...
Hikaru Hanyu, Eitaro Aihara, Miki Tadaishi   +2 more
exaly   +3 more sources

Production of tissue-engineered intestine from expanded enteroids [PDF]

Journal of Surgical Research, 2016
Short bowel syndrome is a life-threatening condition with few solutions. Tissue-engineered intestine (TEI) is a potential treatment, but donor intestine is a limiting factor. Expanded epithelial surrogates termed enteroids may serve as a potential donor source.To produce TEI from enteroids, crypts were harvested from mice and enteroid cultures ...
Jed Johnson, Gail E Besner
exaly   +3 more sources

Enteroids for Nutritional Studies

Molecular Nutrition & Food Research, 2019
AbstractNutritional studies are greatly hampered by a paucity of proper models. Previous studies on nutrition have employed conventional cell lines and animal models to gain a better understanding of the field. These models lack certain correlations with human physiological responses, which impede their applications in this field.
Yin, YB (Yuebang), de Jonge, Hugo, Wu, X, Yin, YL   +3 more
openaire   +3 more sources

Establishment of MDR1-knockout human enteroids for pharmaceutical application

Drug Metabolism and Pharmacokinetics, 2023
In the drug development process, it is important to assess the contributions of drug-metabolizing enzymes and/or drug transporters to the intestinal pharmacokinetics of candidate compounds. For such assessments, chemical inhibitors are often used in in vitro systems.
Tatsuya Inui, Ryuga Nomoto, Jumpei Yokota, Tomoki Yamashita, Kentaro Okada, Wataru Kishimoto, Hiroshi Nakase, Hiroyuki Mizuguchi   +7 more
openaire   +2 more sources

Evaluating protective effects of botanicals under inflammation and oxidative stress in chicken apical-out enteroids [PDF]

Poultry Science, 2023
: Botanicals (BOTs) are well known for their anti-inflammatory and antioxidant activities. They have been widely used as feed additives to reduce inflammation and improve intestinal functions in agricultural animals.
Federico Ghiselli, Liang-En Yu, Andrea Piva   +2 more
exaly   +2 more sources

Generation and Quantitative Imaging of Enteroid Monolayers

2020
The intestinal epithelium is a single layer of cells that plays a critical role in digestion, absorbs nutrients from food, and coordinates the delicate interplay between microbes in the gut lumen and the immune system. Epithelial homeostasis is crucial for maintaining health; disruption of homeostasis results in disorders including inflammatory bowel ...
Laura E, Sanman, Ina W, Chen, Jake M, Bieber, Curtis A, Thorne, Lani F, Wu, Steven J, Altschuler   +5 more
openaire   +2 more sources