Results 1 to 10 of about 1,860,594 (340)

Conductimetric Enzyme Assays [PDF]

European Journal of Biochemistry, 1971
A direct reading, six channel conductivity recording apparatus has been constructed and used to assay enzymes, including some of those found in muscle. This paper gives the essential theory of the method, and indicates that it is a highly versatile assay system. It may be applicable whenever ionic substrates are involved, but this is more easily tested
Anthony J. Lawrence
openaire   +5 more sources

The BhbA Enzyme Assay

Bio-Protocol, 2014
Reductive dehalogenation has been found primarily in anaerobic communities and is originally thought to rarely occur in aerobes. A reductive dehalogenase (BhbA) was characterized from an aerobic strain of Comamonas sp.
Kai Chen, Jiandong Jiang
doaj   +3 more sources

LDH-A Enzyme Assay

Bio-Protocol, 2013
LDH (Lactate dehydrogenase) enzyme catalyzes the reversible conversion of pyruvate to lactate using NAD+ as a cofactor. Although the physiological significance of lactate accumulation in tumor cells, a dead-end product in cellular metabolism, is ...
Di Zhao, Yue Xiong, Qun-Ying Lei, Kun‐Liang Guan   +3 more
doaj   +3 more sources

Automation of enzyme assays. [PDF]

Journal of Clinical Pathology, 1970
A. W. Skillen
openaire   +4 more sources

Biochemical and computational study of an alginate lyase produced by Pseudomonas aeruginosa strain S21 [PDF]

Iranian Journal of Basic Medical Sciences, 2020
Objective(s): Alginates play a key role in mucoid Pseudomonas aeruginosa colonization, biofilm formation, and driving out of cationic antibiotics. P. aeruginosa alginate lyase (AlgL) is a periplasmic enzyme that is necessary for alginate synthesis and ...
Firoozeh Piroozmand, Parinaz Ghadam, Mahboobe Zarrabi, Ahya Abdi Ali   +3 more
doaj   +1 more source

Neuronal ceroid lipofuscinoses in children

Annals of Indian Academy of Neurology, 2021
Background: The neuronal ceroid lipofuscinoses (NCL) constitute a group of gray matter neurodegenerative disorders characterized by the accumulation of ceroid lipopigment in lysosomes in neurons and other cell types.
Mahesh Kamate, Narendranadha Reddy, Mayank Detroja, Virupaxi Hattiholi   +3 more
doaj   +1 more source

Development of an Enzyme-Based Thin-Layer Chromatographic Assay for the Detection of Cyclooxygenase-2 Inhibitors

Separations, 2022
The search for new anti-inflammatory drugs with less side effects requires simple, fast and reliable screening methods. In this context, we have developed a sensitive thin-layer chromatographic (TLC) assay on silica gel plates to detect cyclooxygenase-2 (
Aurélie Urbain, Nidhal Trabelssi, Valérie Bardot   +2 more
doaj   +1 more source

Biochemical and Biophysical Characterisation of the Hepatitis E Virus Guanine-7-Methyltransferase

Molecules, 2022
Hepatitis E virus (HEV) is an understudied pathogen that causes infection through fecal contaminated drinking water and is prominently found in South Asian countries.
Preeti Hooda, Mohd Ishtikhar, Shweta Saraswat, Pooja Bhatia, Deepali Mishra, Aditya Trivedi, Rajkumar Kulandaisamy, Soumya Aggarwal, Manoj Munde, Nemat Ali, Abdullah F. AlAsmari, Mohd A. Rauf, Krishna K. Inampudi, Deepak Sehgal   +13 more
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Insecticidal and Enzyme Inhibition Activities of Leaf/Bark Extracts, Fractions, Seed Oil and Isolated Compounds from Triadica sebifera (L.) Small against Aphis craccivora Koch

Molecules, 2022
Aphid, Aphis craccivora Koch (Hemiptera: Aphididae), is a major sap-sucking insect pest of leguminous crops and also transmits plant viruses, leading to economic yield loss.
Shudh Kirti Dolma, Prithvi Pal Singh, Sajjalavarahalli G. Eswara Reddy   +2 more
doaj   +1 more source

Enzymatic Analysis of Yeast Cell Wall-Resident GAPDH and Its Secretion

mSphere, 2020
In yeast, many proteins are found in both the cytoplasmic and extracellular compartments, and consequently it can be difficult to distinguish nonconventional secretion from cellular leakage.
Michael J. Cohen, Brianne Philippe, Peter N. Lipke   +2 more
doaj   +1 more source