Results 1 to 10 of about 4,652,438 (262)

Conductimetric Enzyme Assays [PDF]

open access: bronzeEuropean Journal of Biochemistry, 1971
A direct reading, six channel conductivity recording apparatus has been constructed and used to assay enzymes, including some of those found in muscle. This paper gives the essential theory of the method, and indicates that it is a highly versatile assay system. It may be applicable whenever ionic substrates are involved, but this is more easily tested
Anthony J. Lawrence
openalex   +5 more sources

The BhbA Enzyme Assay

open access: yesBio-Protocol, 2014
Reductive dehalogenation has been found primarily in anaerobic communities and is originally thought to rarely occur in aerobes. A reductive dehalogenase (BhbA) was characterized from an aerobic strain of Comamonas sp.
Kai Chen, Jiandong Jiang
doaj   +3 more sources

LDH-A Enzyme Assay

open access: yesBio-Protocol, 2013
LDH (Lactate dehydrogenase) enzyme catalyzes the reversible conversion of pyruvate to lactate using NAD+ as a cofactor. Although the physiological significance of lactate accumulation in tumor cells, a dead-end product in cellular metabolism, is ...
Di Zhao   +3 more
doaj   +3 more sources

A serological assay to detect SARS-CoV-2 seroconversion in humans

open access: yesNature Medicine, 2020
Introduction: SARS-Cov-2 (severe acute respiratory disease coronavirus 2), which causes Coronavirus Disease 2019 (COVID19) was first detected in China in late 2019 and has since then caused a global pandemic. While molecular assays to directly detect the
Fatima Amanat   +2 more
exaly   +3 more sources

Diagnosis of Mucopolysaccharidoses and Mucolipidosis by Assaying Multiplex Enzymes and Glycosaminoglycans

open access: yesDiagnostics, 2021
Mucopolysaccharidoses (MPS) and mucolipidosis (ML II/III) are a group of lysosomal storage disorders (LSDs) that occur due to a dysfunction of the lysosomal hydrolases responsible for the catabolism of glycosaminoglycans (GAGs).
Nivethitha Arunkumar   +14 more
doaj   +1 more source

Heterocyclic Cathinones as Inhibitors of Kynurenine Aminotransferase II—Design, Synthesis, and Evaluation

open access: yesPharmaceuticals, 2021
Kynurenic acid is a neuroprotective metabolite of tryptophan formed by kynurenine aminotransferase (KAT) catalyzed transformation of kynurenine. However, its high brain levels are associated with cognitive deficit and with the pathophysiology of ...
Michal Maryška   +6 more
doaj   +1 more source

Metabolic Conversions by Lactic Acid Bacteria during Plant Protein Fermentations

open access: yesFoods, 2022
To secure a sustainable food supply for the rapidly growing global population, great efforts towards a plant-based diet are underway. However, the use of plant proteins comes with several challenges, such as improvement or removal of undesired flavours ...
Wim Engels   +5 more
doaj   +1 more source

Purification and Crystallographic Analysis of a Novel Cold-Active Esterase (HaEst1) from Halocynthiibacter arcticus

open access: yesCrystals, 2021
This report deals with the purification, characterization, and a preliminary crystallographic study of a novel cold-active esterase (HaEst1) from Halocynthiibacter arcticus.
Sangeun Jeon   +8 more
doaj   +1 more source

Isolation of Peroxisomes from Frozen Liver of Rat by Differential and Iodixanol Gradient Centrifugation

open access: yesSeparations, 2023
In the last decade, research has shown that most diseases are associated with organelle dysfunction in which metabolites play a crucial role or indicate specific processes.
Lina Baz   +3 more
doaj   +1 more source

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