Results 51 to 60 of about 651,887 (304)

Phospholipase C is a novel regulator at the early stages of microspore embryogenesis in Nicotiana tabacum

Plant Signaling & Behavior, 2022
Microspore transfers the developmental fate into embryogenesis in vitro regulated by determinant factors of stress-induced. However, the key regulators of microspore embryogenesis (ME) are still largely undiscovered to reveal the mechanism of cell fate ...
Pan Luo, Aixi Jiang, Yi Zhou, Mingchun Yang, Xiaotong Zhou, Yong Yang, Jun Yu, Xingchun Tang   +7 more
doaj   +1 more source

Design, modeling and synthesis of an in vitro transcription rate regulatory circuit [PDF]

, 2008
This paper describes the design, modeling and realization of a synthetic in vitro circuit that aims at regulating the rate of mRNA transcription. Two DNA templates are designed to interact through their transcripts, creating negative feedback loops that ...
Forsberg, Per-Ola, Franco, Elisa, Murray, Richard M.   +2 more
core   +2 more sources

Inhibiting stearoyl‐CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

FEBS Letters, EarlyView.
Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson, Mackenzie K Herroon, Shane Mecca, Laimar C Garmo, Jacob Lindquist, Shrila Rajendran, Steve M. Patrick, Izabela Podgorski   +7 more
wiley   +1 more source

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

Frontiers in Microbiology, 2017
Laccases are a family of copper-containing oxidases with important applications in bioremediation and other various industrial and biotechnological areas.
Jie Yang, Wenjuan Li, Tzi Bun Ng, Xiangzhen Deng, Juan Lin, Xiuyun Ye   +5 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins

Microbial Cell Factories, 2019
Background The methylotrophic yeast Pichia pastoris is well-known for the production of a broad spectrum of functional types of heterologous proteins including enzymes, antigens, engineered antibody fragments, and next gen protein scaffolds and many ...
Xueyun Zheng, Yimin Zhang, Xinying Zhang, Cheng Li, Xiaoxiao Liu, Ying Lin, Shuli Liang   +6 more
doaj   +1 more source

A new strategy for cellulases application in high temperature industrial scenarios with syringic acid assisting

GCB Bioenergy, 2023
In the process of bioethanol production, more stable and active cellulase in high temperature condition is required. In this study, syringic acid was applied in cellulase hydrolysis system.
Qiuping Ran, Mengtian Huang, Huanan Li, Bixin Chen, Lili He, Xinxin Cui, Jiashu Liu, Zhengbing Jiang   +7 more
doaj   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Design of minibinder proteins as universal antagonists against canine and human TNFα

Communications Biology
In this study, we resolve the complex structure of canine TNFα (cTNFα) trimer bound to nanobody molecules at a resolution of 3.1 Å using cryo-electron microscopy.
Jun Weng, Zhiyong Wu, Banbin Xing, Yang Hu, Xiaoyu Hu, Meng Mei, Jiaxin Xu, Mengqing Lu, Yibin Chen, Lin Wei, Ke Ming, Zhizheng Wang, Zhuang Li, Zigong Wei   +13 more
doaj   +1 more source