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Educational activity of enzyme kinetics in an undergraduate biochemistry course: invertase enzyme as a model [PDF]
Journal of Microbiology & Biology EducationThis article aims to simplify and facilitate the process of practical teaching of enzyme kinetics by utilizing minimal teaching laboratory requirements. Simultaneously, it ensures that students comprehend the enzyme kinetics experiment effectively.
Ibrahim Al-Odat
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ELISA-Based Enzyme Kinetics Assay for Measuring cGAS Activity [PDF]
Bio-ProtocolCyclic GMP–AMP synthase (cGAS) is a key cytosolic double-stranded DNA sensor that activates innate immune responses. Upon binding double-stranded DNA, cGAS undergoes conformational activation and catalyzes the synthesis of the second messenger 2′3 ...
Cécile Fréreux, Philip Howe
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Development and Implementation of a Remote Enzyme Kinetics Laboratory Exercise [PDF]
Journal of Microbiology & Biology Education, 2022 The COVID-19 pandemic imposed many new challenges on educational systems and increased the demand for novel strategies for effectively teaching laboratory skills without in-person instruction and without access to laboratory space, including critical ...
Abhishek Bhattacharjee +3 more
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Analysis of variable-order fractional enzyme kinetics model with time delay [PDF]
Scientific ReportsIn enzymatic reactions, studying reaction rates and mechanisms helps us understand how concentration, temperature, and catalysts influence the speed of chemical transformations.
K. Agilan +3 more
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Laboratory Exercise To Measure Restriction Enzyme Kinetics [PDF]
Journal of Microbiology & Biology Education, 2019 Enzymes are ubiquitous in the fields of biology and microbiology, catalyzing critical reactions and enabling a broad range of biotechnological applications.
Caroline Blassick +4 more
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Asymmetric effect of mechanical stress on the forward and reverse reaction catalyzed by an enzyme. [PDF]
PLoS ONE, 2014 The concept of modulating enzymatic activity by exerting a mechanical stress on the enzyme has been established in previous work. Mechanical perturbation is also a tool for probing conformational motion accompanying the enzymatic cycle.
Collin Joseph +3 more
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On the relationships between the Michaelis–Menten kinetics, reverse Michaelis–Menten kinetics, equilibrium chemistry approximation kinetics, and quadratic kinetics [PDF]
Geoscientific Model Development, 2015 The Michaelis–Menten kinetics and the reverse Michaelis–Menten kinetics are two popular mathematical formulations used in many land biogeochemical models to describe how microbes and plants would respond to changes in substrate abundance.
J. Y. Tang
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Enzyme Kinetics of Organic Contaminant Oxygenations
CHIMIA, 2020 Enzymatic oxygenations initiate biodegradation processes of many organic soil and water contaminants. Even though many biochemical aspects of oxygenation reactions are well-known, quantifying rates of oxidative contaminant removal as well as the extent ...
Charlotte E. Bopp +2 more
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Computational and Structural Biotechnology Journal, 2020 Transport of ligands between bulk solvent and the buried active sites is a critical event in the catalytic cycle of many enzymes. The rational design of transport pathways is far from trivial due to the lack of knowledge about the effect of mutations on ...
Piia Kokkonen +8 more
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BMC Pharmacology and Toxicology, 2021 Background Pharmacokinetics (PK) is the process of absorption, distribution, metabolism and elimination (ADME) of drugs. Some drugs undergo zero-order kinetics (ethyl alcohol), first order kinetics (piroxicam) and mixed order kinetics (ascorbic acid ...
Saganuwan Alhaji Saganuwan
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