Results 11 to 20 of about 1,681,787 (293)

The Kinetics of Enzyme Mixtures. [PDF]

open access: yesMolecular biology research communications, 2014
Even purified enzyme preparations are often heterogeneous. For example, preparations of aspartate aminotransferase or cytochrome oxidase can consist of several different forms of the enzyme. For this reason we consider how different the kinetics of the reactions catalysed by a mixture of forms of an enzyme must be to provide some indication of the ...
Brown, Simon   +3 more
core   +4 more sources

Enzyme kinetics

open access: yes
In the rapidly evolving field of biomedicine, the integration of physics into medical research and practice has become increasingly pivotal. The intersection of applied physics and biomedicine represents a frontier of innovation, where principles of physics are leveraged to solve complex biological and medical challenges.
Mishra, Deepak, Waoo, Ashwini A.
core   +3 more sources

Steady-State Enzyme Kinetics [PDF]

open access: yes, 2021
Steady-state enzyme kinetics is a cornerstone technique of biochemistry and related sciences since it allows the characterization and quantification of enzyme behaviour.
Lloyd, Matthew
core   +1 more source

Enzyme Kinetics at High Enzyme Concentration

open access: yesBulletin of Mathematical Biology, 2000
We re-visit previous analyses of the classical Michaelis-Menten substrate-enzyme reaction and, with the aid of the reverse quasi-steady-state assumption, we challenge the approximation d[C]/dt approximately 0 for the basic enzyme reaction at high enzyme concentration.
Schnell, S, Maini, P
openaire   +3 more sources

Effect of Water Content on the Thermal Inactivation Kinetics of Horseradish Peroxidase Freeze-Dried from Alkaline pH [PDF]

open access: yes, 2001
The thermal inactivation of horseradish peroxidase freeze-dried from solutions of different pH (8, 10 and 11.5, measured at 25 C) and equilibrated to different water contents was studied in the temperature range from 110 to 150 C.
Oliveira, J. C.   +2 more
core   +1 more source

Phage display-derived inhibitor of the essential cell wall biosynthesis enzyme MurF [PDF]

open access: yes, 2008
Background To develop antibacterial agents having novel modes of action against bacterial cell wall biosynthesis, we targeted the essential MurF enzyme of the antibiotic resistant pathogen Pseudomonas aeruginosa.
Sanschagrin, François   +13 more
core   +1 more source

Stochastic Simulation of the Kinetics of Multiple Interacting Nucleic Acid Strands [PDF]

open access: yes, 2013
DNA nanotechnology is an emerging field which utilizes the unique structural properties of nucleic acids in order to build nanoscale devices, such as logic gates, motors, walkers, and algorithmic structures. Predicting the structure and interactions of a
Schaeffer, Joseph Malcolm
core   +1 more source

Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies

open access: yesJournal of Analytical Methods in Chemistry, 2012
Enzyme kinetics studies normally focus on the initial rate of enzymatic reaction. However, the manual operation of steps of the conventional enzyme kinetics method has some drawbacks.
Supaporn Kradtap Hartwell, Kate Grudpan
doaj   +1 more source

The effect of enzyme treatment on the in vitro fermentation of Lucerne incubated with equine faecal inocula [PDF]

open access: yes, 2005
A series of experiments was conducted to determine the effects of a fibrolytic enzyme preparation (enzyme 1; E1) on the in vitro fermentation of lucerne incubated with equine faecal inocula.
Moore-Colyer, Meriel J. S.   +10 more
core   +1 more source

Mapping of Enzyme Kinetics on a Microfluidic Device. [PDF]

open access: yesPLoS ONE, 2016
A microfluidic platform or "microfluidic mapper" is demonstrated, which in a single experiment performs 36 parallel biochemical reactions with 36 different combinations of two reagents in stepwise concentration gradients.
Hoon Suk Rho   +3 more
doaj   +1 more source

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