Results 121 to 130 of about 10,608 (175)

Mechanical cues regulate <i>in vitro</i> implantation of mouse embryos. [PDF]

Mater Today Bio
Liu Q, Chen K, Pan S, Zhang T, Wang Q, Zhu J, Zhang J, Jiang X, Jia H, Zheng S, Luo T.   +10 more
europepmc   +1 more source

Actin and myosin dynamics during epithelial remodeling in avian gastrulation

Ieda Y, Phan C, Alegria-Prévot O, Villedieu A, Gros J.   +4 more
europepmc   +1 more source

Loss of FANCM impairs primordial germ cell differentiation in vitro

Kaucher AV, Schorle HR, Parreira BR, Sun R, Stewart-Morgan KR, Pinborg A, Hoffmann ER, Halliwell JA.   +7 more
europepmc   +1 more source

Epiblast Morphogenesis Before Gastrulation

Developmental Biology, 2015
The epiblast is a single cell-layered epithelium which generates through gastrulation all tissues in an amniote embryo proper. Specification of the epiblast as a cell lineage in early development is coupled with that of the trophoblast and hypoblast, two lineages dedicated to forming extramebryonic tissues.
Guojun Sheng
exaly   +3 more sources

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Generation of Epiblast-Like Cells

2022
Different states of pluripotency can be captured in vitro depending on the embryo stage from which they are derived and the culture conditions. Pluripotency is a continuum of different states between the two extremes of naïve embryonic stem cells (ESCs) and primed Epiblast Stem Cells (EpiSCs), which resemble the pre/peri- and post- implantation embryo,
Cermola, Federica, Patriarca, Eduardo J., Minchiotti, Gabriella   +2 more
openaire   +3 more sources

Grafting of Epiblast Stem Cell into the Epiblast and Whole-Embryo Imaging to Unveil Lineage Competence

2022
Here we describe a method to engraft epiblast stem cells (EpiSC) into the epiblast of gastrulation-stage mouse embryo to test the lineage propensity acquired by the EpiSCs during in vitro culture under different signaling conditions. After dissection and grafting, the recipient embryos can be grown in whole-embryo culture for up to 48 h and the ...
Pierre, Osteil, Joshua B, Studdert, Patrick P L, Tam   +2 more
openaire   +2 more sources

Manipulating the Avian Epiblast and Epiblast-Derived Stem Cells

2013
Compared to eutherian mammals, birds retain a primitive form of epiblast development. Molecular studies of the avian epiblast can provide valuable insight for mammalian epiblast research. Here, we introduce several basic techniques in handling epiblast-stage embryos of the chick, the major model organism for avian developmental biology studies.
Cantas, Alev, Mikiharu, Nakano, Yuping, Wu, Hiroyuki, Horiuchi, Guojun, Sheng   +4 more
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Culturing the epiblast cells of the pig blastocyst

In Vitro Cellular & Developmental Biology - Animal, 1993
Pig epiblast cells that had been separated from other early embryonic cells were cultured in vitro. A three-step dissection protocol was used to isolate the epiblast from trophectoderm and primitive endoderm before culturing. Blastocysts collected at 7 to 8 days postestrus were immunodissected to obtain the inner cell mass (ICM) and destroy ...
N C, Talbot, C E, Rexroad, V G, Pursel, A M, Powell, N D, Nel   +4 more
openaire   +2 more sources

Generation of Gastruloids from Epiblast-Like Cells

2022
The different states of mouse pluripotency described so far rely on a combination of molecular, phenotypic, and functional analysis. Embryonic Stem cells (ESCs) aggregated in suspension culture are able to form 3D embryo-like structures called gastruloids that mimic features of the gastrulation process.
Cermola, Federica, Patriarca, Eduardo J., Minchiotti, Gabriella   +2 more
openaire   +2 more sources

Isolation and Maintenance of Mouse Epiblast Stem Cells

Methods in Molecular Biology, 2010
Epiblast stem cells (EpiSCs) are isolated from the postimplantation mouse embryo just after implantation but prior to gastrulation. EpiSCs are pluripotent and provide a tractable, in vitro system to study the processes that function during gastrulation to transition pluripotent cells to their differentiated derivatives.
Paul J. Tesar, Josh G Chenoweth
exaly   +3 more sources