Results 121 to 130 of about 10,397 (178)

Correction to "An Aggregation of Human Embryonic and Trophoblast Stem Cells Reveals the Role of Trophectoderm on Epiblast Differentiation". [PDF]

Cell Prolif
europepmc   +1 more source

Cell-context response to germ layer differentiation signals is predetermined by the epigenome in regionalized epiblast populations. [PDF]

Nat Commun
Menezes NA, Peterson KJ, Guo X, Castiglioni V, Kalvisa A, Filimonow K, Schachter K, Schuh CM, Pasias A, Mariani L, Brickman JM, Sedzinski J, Ferretti E.   +12 more
europepmc   +1 more source

A toolkit for mapping cell identities in relation to neighbors reveals conserved patterning of neuromesodermal progenitor populations. [PDF]

PLoS Biol
French M, Migueles RP, Neaverson A, Chakraborty A, Pettini T, Steventon B, Clark E, Dale JK, Blin G, Wilson V, Lowell S.   +10 more
europepmc   +1 more source

Epiblast morphogenesis before gastrulation

Developmental Biology, 2015
The epiblast is a single cell-layered epithelium which generates through gastrulation all tissues in an amniote embryo proper. Specification of the epiblast as a cell lineage in early development is coupled with that of the trophoblast and hypoblast, two lineages dedicated to forming extramebryonic tissues.
Guojun Sheng
exaly   +3 more sources

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Generation of Epiblast-Like Cells

2022
Different states of pluripotency can be captured in vitro depending on the embryo stage from which they are derived and the culture conditions. Pluripotency is a continuum of different states between the two extremes of naïve embryonic stem cells (ESCs) and primed Epiblast Stem Cells (EpiSCs), which resemble the pre/peri- and post- implantation embryo,
Cermola, Federica, Patriarca, Eduardo J., Minchiotti, Gabriella   +2 more
openaire   +3 more sources

Grafting of Epiblast Stem Cell into the Epiblast and Whole-Embryo Imaging to Unveil Lineage Competence

2022
Here we describe a method to engraft epiblast stem cells (EpiSC) into the epiblast of gastrulation-stage mouse embryo to test the lineage propensity acquired by the EpiSCs during in vitro culture under different signaling conditions. After dissection and grafting, the recipient embryos can be grown in whole-embryo culture for up to 48 h and the ...
Pierre, Osteil, Joshua B, Studdert, Patrick P L, Tam   +2 more
openaire   +2 more sources

Manipulating the Avian Epiblast and Epiblast-Derived Stem Cells

2013
Compared to eutherian mammals, birds retain a primitive form of epiblast development. Molecular studies of the avian epiblast can provide valuable insight for mammalian epiblast research. Here, we introduce several basic techniques in handling epiblast-stage embryos of the chick, the major model organism for avian developmental biology studies.
Cantas, Alev, Mikiharu, Nakano, Yuping, Wu, Hiroyuki, Horiuchi, Guojun, Sheng   +4 more
openaire   +2 more sources

Culturing the epiblast cells of the pig blastocyst

In Vitro Cellular & Developmental Biology - Animal, 1993
Pig epiblast cells that had been separated from other early embryonic cells were cultured in vitro. A three-step dissection protocol was used to isolate the epiblast from trophectoderm and primitive endoderm before culturing. Blastocysts collected at 7 to 8 days postestrus were immunodissected to obtain the inner cell mass (ICM) and destroy ...
N C, Talbot, C E, Rexroad, V G, Pursel, A M, Powell, N D, Nel   +4 more
openaire   +2 more sources

Generation of Gastruloids from Epiblast-Like Cells

2022
The different states of mouse pluripotency described so far rely on a combination of molecular, phenotypic, and functional analysis. Embryonic Stem cells (ESCs) aggregated in suspension culture are able to form 3D embryo-like structures called gastruloids that mimic features of the gastrulation process.
Cermola, Federica, Patriarca, Eduardo J., Minchiotti, Gabriella   +2 more
openaire   +2 more sources

Adhesion and fusion of the extraembryonic epiblast

Tissue and Cell, 1989
A tissue culture model system has been devised to examine the attachment, expansion, and fusion of epithelial cell sheets. A normal embryonic epithelial tissue, the extraembryonic epiblast of the chick, is isolated mechanically and cultured on its natural substratum, the vitelline membrane.
openaire   +2 more sources