Results 71 to 80 of about 33,348 (245)

Tracing back variations in archaeal ESCRT-based cell division to protein domain architectures.

PLoS ONE, 2022
The Endosomal Sorting Complex Required for Transport (ESCRT) system is a multi-protein machinery that is involved in cell division of both Eukaryotes and Archaea.
Béla P Frohn, Tobias Härtel, Jürgen Cox, Petra Schwille   +3 more
doaj   +1 more source

Isolation Defines Identity: Functional Consequences of Extracellular Vesicle Purification Strategies

Advanced Healthcare Materials, EarlyView.
Four extracellular vesicle purification strategies are compared using ovarian‐cancer ascites and ES‐2 cell supernatants. A novel workflow links purification to function by combining particle‐normalized proteomics with matched cell‐free and cell‐based assays.
Christian Preußer, Dolores J. Salander, Witold Szymanski, Johannes Graumann, Yukai Wang, Kai Zhao, Jörg W. Bartsch, María Gómez‐Serrano, Daniel Bachurski, Silke Reinartz, Elke von Pogge von Strandmann   +10 more
wiley   +1 more source

Structural and Functional Characterization of EXPO‐Derived Extracellular Vesicles in Plants

Advanced Science, EarlyView.
In this study, 3D electron tomography (ET), cryo‐ET, and immunogold transmission electron microscopy (TEM) are employed to characterize plant extracellular vesicles (EVs) under physiological conditions. EVs are classified into three distinct categories according to their size, content, and molecular‐marker profiles. Furthermore, Exo70E2‐positive medium
Jiayang Gao, Yanbin Li, Shengqi Zhang, Yilin He, Zhiqi Liu, Juan Wang, Jingmin Hua, Jinyu Chen, Junru Zhong, Huan Zhong, Yiji Xia, Yong Cui, Liwen Jiang   +12 more
wiley   +1 more source

Super-Resolution Microscopy: A Virus’ Eye View of the Cell [PDF]

, 2014
It is difficult to observe the molecular choreography between viruses and host cell components, as they exist on a spatial scale beyond the reach of conventional microscopy.
Grove, J
core   +2 more sources

EGF receptor trafficking: consequences for signaling and cancer [PDF]

, 2013
The ligand-stimulated epidermal growth factor receptor (EGFR) has been extensively studied in the analysis of molecular mechanisms regulating endocytic traffic and the role of that traffic in signal transduction.
Eden, ER, Futter, CE, Tomas, A
core   +1 more source

HRS Degradation‐Induced Nicotinamide Deficiency in Placental Extracellular Vesicles Triggers Preeclampsia by Disrupting Maternal‐Fetal Immune Homeostasis

Advanced Science, EarlyView.
This study shows that lower NAM levels in PE‐derived pEVs correlate with disease severity. NAM‐deficient pEVs reduce Th1 and Th17 inhibition, leading to PE‐like symptoms. NAM in pEVs inhibits Th1 via SIRT1 and Th17 via macrophages. Reduced NAM in PE‐EVs is due to decreased HRS expression in trophoblasts, resulting from elevated HSP27.
Haiyi Fei, Yuhan Lin, Xiu Liu, Xiaohong Zhu, Xiaowen Lu, Zhan Shi, Fan Jia, Shiqian Xu, Ling Fang, Lingling Jiang, Songying Zhang   +10 more
wiley   +1 more source

Genome-wide screen reveals important roles for ESCRT proteins in drug/ion resistance of fission yeast. [PDF]

PLoS ONE, 2018
To study sodium homeostasis, we performed a genome-wide screen for deletion strains that show resistance to NaCl. We identified 34 NaCl-resistant strains.
Yikun Yang, Qiannan Liu, Guanglie Jiang, Si Chen, Lina Zhou, Norihiro Sakamoto, Takayoshi Kuno, Yue Fang, Fan Yao   +8 more
doaj   +1 more source

Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation

Nature Communications
Autophagy is relevant for diverse processes in eukaryotic cells, making its regulation of fundamental importance. The formation and maturation of autophagosomes require a complex choreography of numerous factors.
Niccolò Mosesso, Niharika Savant Lerner, Tobias Bläske, Felix Groh, Shane Maguire, M. L. Niedermeier, Eliane Landwehr, Karin Vogel, Konstanze Meergans, Marie-Kristin Nagel, M. Drescher, Florian Stengel, Karin Hauser, Erika Isono   +13 more
semanticscholar   +1 more source

In vitro reconstitution of calcium-dependent recruitment of the human ESCRT machinery in lysosomal membrane repair

bioRxiv, 2022
The endosomal sorting complex required for transport (ESCRT) machinery has been shown to be centrally involved in repair of damage to both the plasma and lysosome membranes.
Sankalp Shukla, K. Larsen, Chen-Yin Ou, Kevin Rose, J. Hurley   +4 more
semanticscholar   +1 more source

A novel minimal in vitro system for analyzing HIV-1 Gag mediated budding

, 2014
A biomimetic minimalist model membrane was used to study the mechanism and kinetics of cell-free in vitro HIV-1 Gag budding from a giant unilamellar vesicle (GUV).
Gill, Sarjeet, Gui, Dong, Gupta, Sharad, Huang, I-Chueh, Mohideen, Umar, Rao, A. L. N., Xu, Jun, Zandi, Roya   +7 more
core   +1 more source