Results 1 to 10 of about 265,040 (167)

The Specificity of Transgene Suppression in Plants by Exogenous dsRNA [PDF]

Plants, 2022
The phenomenon of RNA interference (RNAi) is widely used to develop new approaches for crop improvement and plant protection. Recent investigations show that it is possible to downregulate plant transgenes, as more prone sequences to silencing than ...
Alexandra S Dubrovina, Olga Aleynova, Konstantin Kiselev   +2 more
exaly   +5 more sources

Recent Advances in the Development of Exogenous dsRNA for the Induction of RNA Interference in Cancer Therapy [PDF]

Molecules, 2021
Selective regulation of gene expression by means of RNA interference has revolutionized molecular biology. This approach is not only used in fundamental studies on the roles of particular genes in the functioning of various organisms, but also possesses ...
Konstantin Orishchenko
exaly   +5 more sources

Exogenous dsRNA-Mediated RNAi: Mechanisms, Applications, Delivery Methods and Challenges in the Induction of Viral Disease Resistance in Plants [PDF]

Viruses
The increasing challenges posed by plant viral diseases demand innovative and sustainable management strategies to minimize agricultural losses. Exogenous double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) represents a transformative approach ...
Venu Emmadi, Namburi Karunakar Reddy, Bhukya Srinivas   +2 more
exaly   +5 more sources

Impact of Exogenous dsRNA on miRNA Composition in Arabidopsis thaliana [PDF]

Plants
The application of double-stranded RNAs (dsRNAs) to plant surfaces has emerged as a promising tool for manipulating gene expression in plants and pathogens, offering new opportunities for crop improvement.
Nikolay N. Nityagovsky, Konstantin V. Kiselev, Andrey R. Suprun, Alexandra S. Dubrovina   +3 more
doaj   +3 more sources

Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperda [PDF]

Frontiers in Physiology, 2022
RNAi is regarded as a promising technology for pest control. However, not all insects are sensitive to RNAi. Studies have confirmed that insect dsRNases are one of key factors affecting RNAi efficiency.
Yang Yao, Dong-Jiang Lin, Xiang-Yun Cai, Ran Wang, You-Ming Hou, Chao-Hua Hu, San-Ji Gao, Jin-Da Wang   +7 more
doaj   +2 more sources

Silencing of the Nicotiana benthamiana phytoendesaturase gene by root treatment of exogenous dsRNA [PDF]

Вавиловский журнал генетики и селекции
RNA interference (RNAi) is a powerful tool for gene silencing. It has recently been used to design promising plant protection strategies against pests such as viruses, insects, etc.
T. S. Golubeva, V. A. Cherenko, E. A. Filipenko, I. V. Zhirnov, A. A. Ivanov, A. V. Kochetov   +5 more
doaj   +2 more sources

Exogenous dsRNA made accessible to Dicer by two eukaryotic RNA-dependent RNA polymerases in Paramecium tetraurelia [PDF]

Communications Biology
Discrimination of self from non-self RNA is a critical requirement for any cell to respond to infections and to maintain cellular integrity. We report novel functions for two RNA-dependent RNA polymerases (RDRs) in Paramecium.
Marcello Pirritano, Johannes Buescher, Pauline Staubach, Thorsten Tacken, Yulia Yakovleva, Mark Sabura, Kristela Shehu, Sören Franzenburg, Marc Schneider, Martin Simon   +9 more
doaj   +2 more sources

RNAi-based pest control: Production, application and the fate of dsRNA

Frontiers in Bioengineering and Biotechnology, 2022
The limitations of conventional pesticides have raised the demand for innovative and sustainable solutions for plant protection. RNA Interference (RNAi) triggered by dsRNA has evolved as a promising strategy to control insects in a species-specific ...
Li He, Li He, Li He, Yanna Huang, Yanna Huang, Yanna Huang, Xueming Tang, Xueming Tang, Xueming Tang   +8 more
exaly   +3 more sources

RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops. [PDF]

Adv Sci (Weinh)
This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Zhang X, Wu Y, Hou J, Gao L, Pan L, Shen C, Zhao C, Gao F, Zhang Y, Ren L, Fang X, Dun X, Liu S, Liu L.   +13 more
europepmc   +2 more sources

BcMFSR Is Required for Efficient Uptake of Exogenous dsRNA and Spray-Induced Gene Silencing in Botrytis cinerea. [PDF]

Mol Plant Pathol
Spray‐induced gene silencing (SIGS) uses exogenous dsRNA to silence pathogen genes and control grey mould disease. We identify BcMFSR, an MFS protein required for dsRNA uptake and SIGS efficacy in Botrytis cinerea. Loss of BcMFSR abolishes dsRNA internalization and disease suppression, revealing a novel role for MFS proteins.
Xu L, Yu Z, Du R, Ma H, Hu X, Wei R, Hu Q, Sheng C, Njoki WD, Chen P, Shi X, Tang C, Zhang H, Zhou S, Zhong H, Wang W, Umida I, Zhao H.   +17 more
europepmc   +2 more sources