Delivery, Persistence, and Functional Response of dsRNA in Acyrthosiphon pisum: Progress and Remaining Barriers. [PDF]
Arch Insect Biochem PhysiolAphids efficiently ingest dsRNA, which persists in the midgut but is rapidly degraded by aphid nucleases when unprotected. Chitosan and LDH‐based formulations improve dsRNA stability, triggering RNAi activation. Despite this protection, gene knockdown remains variable, though the RNAi machinery is consistently activated.
Merkel L +4 more
europepmc +2 more sources
Communications BiologyRNA-binding proteins (RBPs) provide a critical post-transcriptional regulatory layer in determining RNA fate. Currently, UV crosslinking followed by oligo-dT pull-down is the gold standard in identifying the RBP repertoire of poly-adenylated RNAs, but ...
JinA Lim +12 more
doaj +2 more sources
Reduction in Musca domestica fecundity by dsRNA-mediated gene knockdown. [PDF]
PLoS ONE, 2018 House flies (Musca domestica) are worldwide agricultural pests with estimated control costs at $375 million annually in the U.S. Non-target effects and widespread resistance challenge the efficacy of traditional chemical control.
Neil D Sanscrainte +7 more
doaj +2 more sources
Exogenous Application of dsRNA for the Control of Viruses in Cucurbits
Frontiers in Plant Science, 2022 The recurrent emergence of viral diseases in intensive horticultural crops requires alternative control strategies. The topical application of double-stranded RNA (dsRNA) molecules homologous to pathogens has been proposed as a tool for virus control in ...
Leonardo Velasco, Dirk Janssen
exaly +3 more sources
Exogenous dsRNA Induces RNA Interference of a Chalcone Synthase Gene in Arabidopsis thaliana. [PDF]
Int J Mol Sci, 2022 Recent investigations have shown the possibility of artificial induction of RNA interference (RNAi) via plant foliar treatments with naked double-stranded RNA (dsRNA) to silence essential genes in plant fungal pathogens or to target viral RNAs ...
Nityagovsky NN +3 more
europepmc +2 more sources
Interactional Endocytosis and Transmembrane Transport Promote Cellular Internalization of Nano-Delivered RNA Drugs for Efficient Control of Crop Diseases. [PDF]
Plant Biotechnol JABSTRACT Botrytis cinerea is a widespread plant pathogenic ascomycete that causes grey mould in over 1400 species and impacts global crop yields. Double‐stranded RNA (dsRNA)‐induced gene silencing is a promising technology for pest control, but efficient delivery remains a major challenge.
Guan M +7 more
europepmc +2 more sources
A model system test of trophic transmission of RNA interference effects using the milkweed bug Oncopeltus fasciatus (Hemiptera) [PDF]
Scientific ReportsDesigning species-specific dsRNAs makes gene knockdown by RNA interference (RNAi) ideal for pest control. Here, we establish the milkweed bug Oncopeltus fasciatus as a lab model to assess RNAi impacts.
Ebony Michelle Argaez +5 more
doaj +2 more sources
Soil-free bioassays for testing novel control agents against Phytophthora cinnamomi root rot [PDF]
Frontiers in Plant SciencePhytophthora cinnamomi is considered as one of the world’s worst plant pathogens, infecting about 5,000 plant species including those of agricultural and environmental significance.
Leny Jane Pame +7 more
doaj +2 more sources
Orchestrating innate immunity through RNA editing and helicase activity: ADAR1, dsRNA sensors, and tumor immune evasion [PDF]
Frontiers in Cell and Developmental BiologyDysregulated dsRNA editing and RNA metabolism in cancer contribute to immune evasion, highlighting the critical role of RNA structural regulation in disease.
Moeko Minakuchi +2 more
doaj +2 more sources
PlantsRNA interference (RNAi) is a regulatory and protective mechanism that plays a crucial role in the growth, development, and control of plant responses to pathogens and abiotic stresses.
Andrey R. Suprun +4 more
doaj +2 more sources