Results 11 to 20 of about 22,111 (197)

RNAi-based pest control: Production, application and the fate of dsRNA

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
The limitations of conventional pesticides have raised the demand for innovative and sustainable solutions for plant protection. RNA Interference (RNAi) triggered by dsRNA has evolved as a promising strategy to control insects in a species-specific ...
Li He   +8 more
doaj   +3 more sources

Exogenous Application of dsRNA for the Control of Viruses in Cucurbits

open access: yesFrontiers in Plant Science, 2022
The recurrent emergence of viral diseases in intensive horticultural crops requires alternative control strategies. The topical application of double-stranded RNA (dsRNA) molecules homologous to pathogens has been proposed as a tool for virus control in ...
Josemaría Delgado-Martín   +4 more
doaj   +3 more sources

Physiological Conditions and dsRNA Application Approaches for Exogenously induced RNA Interference in Arabidopsis thaliana [PDF]

open access: yesPlants, 2021
Recent studies have revealed that foliar application of double-stranded RNAs (dsRNAs) or small-interfering RNAs (siRNAs) encoding specific genes of plant pathogens triggered RNA interference (RNAi)-mediated silencing of the gene targets.
Konstantin V. Kiselev   +4 more
doaj   +3 more sources

Nanovector-mediated exogenous delivery of dsRNA induces silencing of target genes in very young tomato flower buds

open access: yesNanoscale Advances, 2022
We propose a method to deliver nanoparticle-coupled dsRNA to tomato flower buds to silence endogenous genes.
Barbara Molesini   +2 more
exaly   +3 more sources

Exogenous dsRNA Induces RNA Interference of a Chalcone Synthase Gene in Arabidopsis thaliana. [PDF]

open access: yesInt J Mol Sci, 2022
Recent investigations have shown the possibility of artificial induction of RNA interference (RNAi) via plant foliar treatments with naked double-stranded RNA (dsRNA) to silence essential genes in plant fungal pathogens or to target viral RNAs. Furthermore, several studies have documented the downregulation of plant endogenous genes via external ...
Nityagovsky NN   +3 more
europepmc   +3 more sources

Knockdown of Bmp1 and Pls1 Virulence Genes by Exogenous Application of RNAi-Inducing dsRNA in Botrytis cinerea

open access: yesInternational Journal of Molecular Sciences, 2023
Botrytis cinerea is a pathogen of wide agronomic and scientific importance partly due to its tendency to develop fungicide resistance. Recently, there has been great interest in the use of RNA interference as a control strategy against B. cinerea. In order to reduce the possible effects on non-target species, the sequence-dependent nature of RNAi can ...
Maria Spada   +2 more
exaly   +3 more sources

Feeding exogenous dsRNA interferes with endogenous sRNA accumulation in Paramecium. [PDF]

open access: yesDNA Res, 2020
AbstractSupply of exogenous dsRNA (exo-dsRNA), either by injection or by feeding, is a fast and powerful alternative to classical knockout studies. The biotechnical potential of feeding techniques is evident from the numerous studies focusing on oral administration of dsRNA to control pests and viral infection in crops/animal farming.
Karunanithi S   +7 more
europepmc   +5 more sources

Cellular dsRNA interactome captured by K1 antibody reveals the regulatory map of exogenous RNA sensing

open access: yesCommunications Biology
RNA-binding proteins (RBPs) provide a critical post-transcriptional regulatory layer in determining RNA fate. Currently, UV crosslinking followed by oligo-dT pull-down is the gold standard in identifying the RBP repertoire of poly-adenylated RNAs, but ...
JinA Lim   +12 more
doaj   +3 more sources

Exogenous Application of dsRNA—Inducing Silencing of the Fusarium oxysporum Tup1 Gene and Reducing Its Virulence

open access: yesInternational Journal of Molecular Sciences
Fusarium oxysporum is a widespread soil-borne fungal pathogen that can infect various plants, causing wilt and root rot diseases. The root rot disease of Atractylodes macrocephala caused by F. oxysporum is among the most serious diseases associated with continuous cropping, significantly hindering its sustainable development. In this study, we aimed to
Fan, Sen   +8 more
exaly   +3 more sources

Cellular uptake of extracellular dsRNA is tissue-dependent in insects [PDF]

open access: yesBMC Biology
Background RNA interference (RNAi), a naturally occurring gene silencing mechanism found in almost all eukaryotic organisms, has proven to be an adaptable and powerful tool in therapeutics, bioengineering, and agriculture. Differential responses to RNAi,
Xuekai Shi   +9 more
doaj   +2 more sources

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