Results 11 to 20 of about 3,039 (115)
Crystallographic Studies of Bacterial Exoribonucleases [PDF]
The Scientific World JOURNAL, 2002 INTRODUCTION. Ribonucleases (RNases) play a central role in all cellular RNA processes. These processes include mRNA degradation, and maturation and turnover of stable RNAs, which are vital for the proper functioning of all cells. E. coli has served as a model system for understanding the role of ribonucleases in RNA metabolism, and eight distinct ...
Tristan J. Fiedler, Arun Malhotra
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Real-time fluorescence detection of exoribonucleases [PDF]
RNA, 2009 The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic RNA–DNA hybrid is a powerful tool for a real-time fluorescence detection and assay of exoribonucleases (RT-FeDEx).
Sinturel, F. +5 more
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Immunosuppressive arenaviral exoribonuclease
Oncotarget, 2015 Arenaviruses cause up to 500,000 zoonotic infections per year in endemic areas of Africa and South America and can lead to severe and lethal hemorrhagic fever (HF) symptoms [1]. Currently, there is no specific antiviral drug or vaccine available for the treatment of these infections, with the exception of the Candid #1 vaccine that has successfully ...
Bjoern, Meyer, Hinh, Ly
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Exoribonucleases as Modulators of Virulence in Pathogenic Bacteria [PDF]
Frontiers in Cellular and Infection Microbiology, 2012 Pathogenic bacteria are responsible for severe diseases worldwide. RNA stability is a major player controlling the expression of virulence factors. Ribonucleases (RNases) are the enzymes responsible for the maturation and degradation of RNA molecules (Arraiano et al., 2010; Silva et al., 2011).
Matos, Rute G. +4 more
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Biochemical Characterization of Exoribonuclease Encoded by SARS Coronavirus [PDF]
BMB Reports, 2007 The nsp14 protein is an exoribonuclease that is encoded by severe acute respiratory syndrome coronavirus (SARS-CoV). We have cloned and expressed the nsp14 protein in Escherichia coli, and characterized the nature and the role(s) of the metal ions in the reaction chemistry.
Ping, Chen +5 more
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Characterization of a bafinivirus exoribonuclease activity
Journal of General Virology, 2018 White bream virus (WBV), a poorly characterized plus-strand RNA virus infecting freshwater fish of the Cyprinidae family, is the prototype species of the genus Bafinivirus in the subfamily Torovirinae (family Coronaviridae, order Nidovirales). In common with other nidoviruses featuring >20 kilobase genomes, bafiniviruses have been predicted to encode ...
Izabela, Durzynska +4 more
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Mutational analysis of exoribonuclease I from Saccharomyces cerevisiae [PDF]
Nucleic Acids Research, 1998 Exoribonuclease I from yeast is a 175 kDa protein that is responsible for the majority of cytoplasmic mRNA degradation. Alignment of the Xrn1p sequence with homologs from yeast as well as from higher eukaryotes suggests that the protein is composed of several domains: two acidic N-terminal domains which likely contain the exonuclease, a basic middle ...
A M, Page +4 more
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XRN 5′→3′ exoribonucleases: Structure, mechanisms and functions [PDF]
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2013 The XRN family of 5'→3' exoribonucleases is critical for ensuring the fidelity of cellular RNA turnover in eukaryotes. Highly conserved across species, the family is typically represented by one cytoplasmic enzyme (XRN1/PACMAN or XRN4) and one or more nuclear enzymes (XRN2/RAT1 and XRN3).
Vinay K, Nagarajan +3 more
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Arenaviridae exoribonuclease presents genomic RNA edition capacity [PDF]
, 2019 AbstractThe Arenaviridae is a large family of viruses causing both acute and persistent infections and causing significant public health concerns in afflicted regions. A “trademark” of infection is the quick and efficient immuno-suppression mediated in part by a 3’-5’ RNA exonuclease domain (ExoN) of the Nucleoprotein (NP).
Yekwa, Elsie +7 more
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Direct observation of processive exoribonuclease motion using optical tweezers [PDF]
Proceedings of the National Academy of Sciences, 2015 Significance Bacteria regulate the synthesis and degradation of RNA molecules to ensure timely and robust responses to an ever-changing environment. The transcript’s lifetime can be influenced profoundly by a secondary structure that can form in the RNA and that may inhibit or promote its digestion by RNases.
Furqan M, Fazal +3 more
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