Results 21 to 30 of about 3,047 (148)
Localization of components of the RNA-degrading machine in Bacillus subtilis
Frontiers in Microbiology, 2016 In bacteria, the control of mRNA stability is crucial to allow rapid adaptation to changing conditions. In most bacteria, RNA degradation is catalyzed by the RNA degradosome, a protein complex composed of endo- and exoribonucleases, RNA helicases and ...
Nora Cascante-Estepa +2 more
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Microorganisms, 2021 Moonlighting proteins are proteins with more than one function. During the past 25 years, they have been found to be rather widespread in bacteria. In Bacillus subtilis, moonlighting has been disclosed to occur via DNA, protein or RNA binding or protein ...
Inam Ul Haq, Sabine Brantl
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A dedicated diribonuclease resolves a key bottleneck for the terminal step of RNA degradation
eLife, 2019 Degradation of RNA polymers, an ubiquitous process in all cells, is catalyzed by specific subsets of endo- and exoribonucleases that together recycle RNA fragments into nucleotide monophosphate.
Soo-Kyoung Kim +8 more
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Synthesis of 5’ end-labeled RNA
Bio-Protocol, 2012 5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P32 labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases generally do not
Harald Putzer
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Nuclease Activity of the Junín Virus Nucleoprotein C-Terminal Domain
Viruses, 2023 The mammarenavirus Junín (JUNV) is the causative agent of Argentine hemorrhagic fever, a severe disease of public health concern. The most abundant viral protein is the nucleoprotein (NP), a multifunctional, two-domain protein with the primary role as ...
Alicia Armella Sierra +6 more
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BMC Genomics, 2018 Background Exoribonucleases are crucial for RNA degradation in Escherichia coli but the roles of RNase R and PNPase and their potential overlap in stationary phase are not well characterized.
Clémentine Dressaire +5 more
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RNase J1 and J2 Are Host-Encoded Factors for Plasmid Replication
Frontiers in Microbiology, 2021 Plasmids need to ensure their transmission to both daughter-cells when their host divides, but should at the same time avoid overtaxing their hosts by directing excessive host-resources toward production of plasmid factors.
Vanessa Andrade Guimarães +10 more
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Cell Reports, 2019 Summary: Cell-based studies of human ribonucleases traditionally rely on methods that deplete proteins slowly. We engineered cells in which the 3′→5′ exoribonucleases of the exosome complex, DIS3 and EXOSC10, can be rapidly eliminated to assess their ...
Lee Davidson +7 more
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PLoS ONE, 2018 Flaviviruses accumulate abundant subgenomic RNA (sfRNA) in infected cells. It has been reported that sfRNA results from stalling of host 5'-to-3' exoribonuclease XRN1 at the highly structured RNA of the 3' untranslated region (UTR).
Yi-Shiuan Chen +4 more
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Biochemical Characterization of Exoribonuclease Encoded by SARS Coronavirus [PDF]
BMB Reports, 2007 The nsp14 protein is an exoribonuclease that is encoded by severe acute respiratory syndrome coronavirus (SARS-CoV). We have cloned and expressed the nsp14 protein in Escherichia coli, and characterized the nature and the role(s) of the metal ions in the reaction chemistry.
Ping, Chen +5 more
openaire +2 more sources