Results 21 to 30 of about 2,321,862 (234)
Selection of reference genes (RGs) for normalization of PCR-gene expression data includes two crucial steps: determination of the between-sample transcriptionally more stable genes, and subsequent choosing of the most suitable genes as internal controls.
Carlos Noceda +2 more
doaj +1 more source
Validation of suitable internal control genes for expression studies in aging. [PDF]
Quantitative data from experiments of gene expression are often normalized through levels of housekeeping genes transcription by assuming that expression of these genes is highly uniform. This practice is being questioned as it becomes increasingly clear
Bacalini, MARIA GIULIA +9 more
core +1 more source
Enzymatic Properties of Recombinant Thermophilic Xylanase from Pseudothermotoga thermarum and Its Application in Beer Brewing [PDF]
This study aimed to investigate the efficient expression of the xylanase gene Pthxyn of Pseudothermotoga thermarum and its application in beer brewing. The recombinant plasmid pET-20b-pthxyn was constructed and expressed in Escherichia coli.
LI Qingfei, LIU Yue, YAN Xing, NIE Xinling, LI Xiangqian, TAN Zhongbiao, SHI Hao
doaj +1 more source
Roundup is a glyphosate-based herbicide (GBH) widely used in agriculture and may cause toxic effects in non-target organisms. Model organisms, as zebrafish, and analysis of gene expression by reverse transcription-quantitative PCR (RT-qPCR) could be used
M.L. Jaramillo +6 more
doaj +1 more source
Friedmann Equation and Stability of Inflationary Higher Derivative Gravity [PDF]
Stability analysis on the De Sitter universe in pure gravity theory is known to be useful in many aspects. We first show how to complete the proof of an earlier argument based on a redundant field equation.
A. Dobado +53 more
core +3 more sources
APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining
Summary: The synapsis of DNA ends is a critical step for the repair of double-strand breaks by non-homologous end joining (NHEJ). This is performed by a multicomponent protein complex assembled around Ku70-Ku80 heterodimers and regulated by accessory ...
Sara De Bragança +9 more
doaj +1 more source
Validation of Suitable Reference Genes for Gene Expression Studies on Yak Testis Development
Testis has an important function in male reproduction. Its development is regulated by a large number of genes. The real-time reserve transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a useful tool to evaluate the gene expression levels ...
Xuelan Zhou +9 more
doaj +1 more source
Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability. [PDF]
Thrombospondins (Thbs) are a family of five secreted matricellular glycoproteins in vertebrates that broadly affect cell-matrix interaction. While Thbs4 is known to protect striated muscle from disease by enhancing sarcolemmal stability through increased
Brody, Matthew J +11 more
core +3 more sources
Effects of Nanoparticles on Double-Stranded RNA Stability in Corn Soil [PDF]
Double-stranded RNA (dsRNA) can potentially be used as a pesticide because these molecules trigger an immune response called RNA interference (RNAi). If the expression of essential genes matching the dsRNA sequence are silenced, then the pest dies.
Reyanldo, Miriam +7 more
core +5 more sources
A number of transcriptome datasets for differential expression (DE) genes have been widely used for understanding organismal biology, but these datasets also contain untapped information that can be used to develop more precise analytical tools. With the
Jiaping Zhao +7 more
doaj +1 more source

