Results 61 to 70 of about 6,818,443 (298)

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Transcriptome-based screening and the optimal reference genes for real-time quantitative PCR in Rehmannia chingii and R. henryi

Biologia Plantarum, 2020
Real time quantitative PCR (qPCR) is a powerful tool for studying the expression of specific genes. The accuracy and reliability of qPCR analysis data require the selection of reference genes with stable expression.
X. ZUO, F.-Q. WANG, X.-R. LI, M.-M. LI
doaj   +1 more source

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in Megalurothrips usitatus (thysanoptera: thripidae)

Frontiers in Physiology, 2023
IntroductionGene expression analysis by reverse transcription quantitative polymerase chain reaction (qRT-PCR) has been widely used in research including insects.
Qingfang Hou, Qingfang Hou, Linlin Yuan, Linlin Yuan, Haifeng Jin, Haifeng Jin, Han Yan, Han Yan, Fen Li, Fen Li, Shaoying Wu, Shaoying Wu   +11 more
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Stability of reference gene expression under biotic conditions.

, 2015
Stability of reference gene expression under biotic conditions.
Blair D. Siegfried (208322), Xiaowei Yang (116246), Huipeng Pan (104844), Keith Bidne (747760), Richard L. Hellmich (9367), Xuguo Zhou (104854)   +5 more
core   +2 more sources

Strategic Optimization of CHO Cell Expression Platforms for Biopharmaceutical Manufacturing

Shengwu huaxue yu shengwu wuli jinzhan
Chinese hamster ovary (CHO) cells are the most established and versatile mammalian expression system for the large-scale production of recombinant therapeutic proteins, owing to their genetic stability, adaptability to serum-free suspension culture, and ...
ZHANG Rui-Ming, LI Meng-Lin, ZHU Hong-Wei, ZHANG Xing-Xiao   +3 more
doaj   +1 more source

Levels and Stability of Expression of Transgenes

, 2010
It is well known that in a given cell, at a particular time, only a fraction of the entire genome is expressed. Expression of a gene, nuclear, or organellar starts with the onset of transcription and ends in the synthesis of the functional protein.
Bandopadhyay, Rajib, Haque, Inamul, Singh, Dharmendra, Mukhopadhyay, Kunal   +3 more
openaire   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Validation of expression stability of housekeeping genes.

, 2016
Validation of expression stability of housekeeping genes.
Qiankun Zhu (755358), Yi Liang (110469), Nan Lv (798889), Wei Pei (390028), Jing Fan (149601), Wanjun Wang (755360), Hongqiang An (3332835), Yihui Cui (169693)   +7 more
core   +1 more source