Results 71 to 80 of about 6,818,443 (298)

Stability of reference gene expression under abiotic conditions.

open access: yes, 2015
Stability of reference gene expression under abiotic conditions.
Blair D. Siegfried (208322)   +5 more
core   +2 more sources

Selection and Validation of Reference Genes for RT-qPCR Analysis of Lipid Biosynthesis-Related Genes in Perilla frutescens

open access: yesPlants
Perilla frutescens is a specialty oilseed crop rich in polyunsaturated fatty acids, particularly α-linolenic acid (ALA), and the molecular regulation of seed lipid metabolism is an active area of research. Reverse-transcription quantitative PCR (RT-qPCR)
Siya Yue   +8 more
doaj   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

open access: yesFEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens   +8 more
wiley   +1 more source

Gene expression stability of the candidate genes analyzed by NormFinder.

open access: yes, 2016
A) Gene expression difference between two groups. Gene expression quantities were transformed from the Cq values by using Relative Expression Software [30]. Vertical bars represent the intragroup variation.
Jiewei Zhang (2833409)   +5 more
core   +1 more source

A variance-stabilizing transformation for gene-expression microarray data [PDF]

open access: yesBioinformatics, 2002
Abstract Motivation: Standard statistical techniques often assume that data are normally distributed, with constant variance not depending on the mean of the data. Data that violate these assumptions can often be brought in line with the assumptions by application of a transformation.
Blythe Durbin   +3 more
openaire   +4 more sources

Reconstructing enzyme evolution by protein engineering

open access: yesFEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler   +2 more
wiley   +1 more source

NormFinder stability results; higher stability values mean unstable expression under evaluated conditions.

open access: yes, 2013
NormFinder stability results; higher stability values mean unstable expression under evaluated conditions.
Paola Romina Amable (453036)   +4 more
core   +1 more source

Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer

open access: yesScientific Reports, 2017
Aberrant expression of long non-coding RNAs (lncRNAs) has been regarded as a critical component in bladder cancer (BC) and lncRNAs have been associated with BC development and progression although their overall expression and functional significance is ...
Anna Katharina Seitz   +16 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

open access: yesFEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj   +3 more
wiley   +1 more source

Refined Characterization of the Expression and Stability of the SMN Gene Products [PDF]

open access: yesThe American Journal of Pathology, 2007
Spinal muscular atrophy (SMA) is characterized by degeneration of lower motor neurons and caused by mutations of the SMN1 gene. SMN1 is duplicated in a homologous gene called SMN2, which remains present in patients. SMN has an essential role in RNA metabolism, but its role in SMA pathogenesis remains unknown.
Vitte, J   +9 more
openaire   +3 more sources

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