Results 151 to 160 of about 1,588,316 (303)

Purification Protocols for Extracellular Vesicles [PDF]

, 2017
This chapter provides a description of some of the standard methods used for the isolation of extracellular vesicles (EVs) from a variety of biological fluids, including cell culture media, urine, plasma and serum. The methods presented include ultracentrifugation, ultrafiltration, proprietary polymer-based reagents, size exclusion chromatography ...
Lane, Rebecca E., Korbie, Darren, Trau, Matt, Hill, Michelle M.   +3 more
openaire   +4 more sources

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Injected extracellular vesicles and other nanoparticles hitchhike on erythrocytes and platelets from circulation towards organ clearance

Extracellular Vesicle
The application of extracellular vesicles (EVs) for targeted delivery of drugs, proteins, and RNA to specific cell types or organs is a promising approach in molecular therapy. However, to achieve efficient and targeted delivery, it is crucial to improve
Svetlana Pavlova, Doste R. Mamand, Daniel W. Hagey, Xiuming Liang, H. Yesid Estupiñán, Wenyi Zheng, Guannan Zhou, Risul Amin, Antje M. Zickler, Scott Bonner-Harris, Zankruti Dave, Miina Ojansivu, Manuela O. Gustafsson, Oliver G. Hayes, Oscar P.B. Wiklander, Manuchehr Abedi‐Valugerdi, Samantha Roudi, Antonin Marquant, Joel Z. Nordin, Molly M. Stevens, André Görgens, Samir EL Andaloussi   +21 more
doaj   +1 more source

Cell surface CD11c as a neutrophil aging marker molecule

FEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki, Fahd Alhamdan, Erik Malm, Marie Bermudez, Samuel Y. Kim, Koichi Yuki   +5 more
wiley   +1 more source

Epithelial organization and cyst lumen expansion require efficient Sec13-Sec31-driven secretion [PDF]

, 2012
Epithelial morphogenesis is directed by interactions with the underlying extracellular matrix. Secretion of collagen and other matrix components requires efficient coat complex II (COPII) vesicle formation at the endoplasmic reticulum. Here, we show that
Schmidt, Katy, Hodgson, Lorna, Stephens, David J, Townley, Anna K   +3 more
core   +1 more source

Elevated TRPP2 promotes malignant behavior of head and neck cancer via enhancing exosome biogenesis

Extracellular Vesicle
Background: Exosomes promote cancer progression by mediating intercellular communication via oncogenic cargos. These vesicles originate as intraluminal vesicles (ILVs) within multivesicular bodies (MVBs), with their secretion dependent on MVB trafficking.
Suwen Bai, Hexing Sun, Xiling Xu, Yuan Wei, Yuxin Zhang, Chenxi Zhang, Qing Peng, Junwei Zhu, Juan Du   +8 more
doaj   +1 more source

Fluorination: A strategy to tune the interactions of molecules and nanoscale systems with biological matter

FEBS Open Bio, EarlyView.
This minireview explores fluorination as a key strategy to optimize therapeutic molecules and nanocarriers. By incorporating different fluorinated motifs—from single atoms to linear and branched chains—drug delivery, cellular uptake, and colloidal stability can be improved and fine‐tuned.
Martina Beccalli, Beatrice Lucia Bona, Francesca Baldelli Bombelli   +2 more
wiley   +1 more source

Discriminating extracellular vesicles by their membranes

Extracellular Vesicle
Extracellular Vesicles (EVs) are increasingly employed for targeted drug delivery and advanced diagnostic assays. However, the diagnostic application of EVs so far only relied on biochemical markers as in the case of prostate cancer.
Carlo Guardiani, Matteo Riboli, Flavio Costa, Massimiliano Bruno, Giulia Rossi, Alberto Giacomello   +5 more
doaj   +1 more source

Rationally engineered polymeric interfaces for selective and dynamic recognition of vesicular agents

FEBS Open Bio, EarlyView.
This review explores how molecularly imprinted polymers can be engineered to recognize complex nanoscale vesicles, including extracellular vesicles and membrane‐enveloped viruses. By tailoring polymer cavities to vesicle surface chemistry and structure, these synthetic receptors support selective detection, separation, and integration with optical ...
Özgecan Erdem, Eylul Gulsen Yilmaz, Selcan Genc, Nil Kılıç, Fatih Inci   +4 more
wiley   +1 more source

Dataset for Characterizing the Extracellular Vesicle Proteomic Landscape of the Human Airway using In vitro Organotypic Multi-Cellular Models

These data represent underlying data generated for the study titled 'Characterizing the Extracellular Vesicle Proteomic Landscape of the Human Airway using In vitro Organotypic Multi-Cellular Models'.
Payton, Alexis, Carberry, Celeste, Vitucci, Eva, Rager, Julia E., McCullough, Shaun   +4 more
core   +1 more source