Results 31 to 40 of about 622,719 (265)
Frontiers in Plant Science, 2017 In vivo markers for F-actin organization and dynamics are extensively used to investigate cellular functions of the actin cytoskeleton, which are essential for plant development and pathogen defense.
Adriana Montes-Rodriguez, Benedikt Kost
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Journal of the Mechanical Behavior of Biomedical Materials, 2010 We use the existing data of force-extension experiments on F-actin molecules tied into knots to compute a value of 0.15 for the static friction coefficient for contact between different parts of the same molecule with itself. This estimate for protein-protein friction is relevant for the stabilization of the 273 known proteins with knots, one percent ...
Kirchner, Helmut, Neukirch, Sébastien
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Cingulin interacts with F‐actin in vitro [PDF]
FEBS Letters, 2001 Cingulin, a M r 140–160 kDa protein of the cytoplasmic plaque of epithelial tight junctions (TJ), interacts in vitro with TJ proteins and myosin. Here we investigated cingulin interaction with actin, using His‐tagged, full‐length Xenopus laevis cingulin expressed in insect cells, and glutathione S‐transferase (GST) fusion proteins of fragments of ...
D'atri, Fabio, Citi, Sandra
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Biomedicines, 2022 Changes in F-actin distribution and cortical F-actin morphology are important for blastocyst developmental competence during embryogenesis. However, the effect of paclitaxel as a microtubule stabilizer on embryonic development in pigs remains unclear. We
Seung-Yeon Joe +4 more
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FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
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Induced Arp2/3 Complex Depletion Increases FMNL2/3 Formin Expression and Filopodia Formation
Frontiers in Cell and Developmental Biology, 2021 The Arp2/3 complex generates branched actin filament networks operating in cell edge protrusion and vesicle trafficking. Here we employ a conditional knockout mouse model permitting tissue- or cell-type specific deletion of the murine Actr3 gene ...
Vanessa Dimchev +14 more
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Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
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Targeting F-actin stress fibers to suppress the dedifferentiated phenotype in chondrocytes
European Journal of Cell BiologyActin is a central mediator of the chondrocyte phenotype. Monolayer expansion of articular chondrocytes on tissue culture polystyrene, for cell-based repair therapies, leads to chondrocyte dedifferentiation.
Mandy M. Schofield +7 more
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Nature Communications, 2023 The phylum Apicomplexa comprises important eukaryotic parasites that invade host tissues and cells using a unique mechanism of gliding motility. Gliding is powered by actomyosin motors that translocate host-attached surface adhesins along the parasite ...
Matthew Martinez +8 more
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An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
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