Results 41 to 50 of about 672,429 (301)

Direct Comparison of the Performance of Commonly Employed In Vivo F-actin Markers (Lifeact-YFP, YFP-mTn and YFP-FABD2) in Tobacco Pollen Tubes

open access: yesFrontiers in Plant Science, 2017
In vivo markers for F-actin organization and dynamics are extensively used to investigate cellular functions of the actin cytoskeleton, which are essential for plant development and pathogen defense.
Adriana Montes-Rodriguez, Benedikt Kost
doaj   +1 more source

Friction of F-actin knots

open access: yesJournal of the Mechanical Behavior of Biomedical Materials, 2010
We use the existing data of force-extension experiments on F-actin molecules tied into knots to compute a value of 0.15 for the static friction coefficient for contact between different parts of the same molecule with itself. This estimate for protein-protein friction is relevant for the stabilization of the 273 known proteins with knots, one percent ...
Kirchner, Helmut, Neukirch, Sébastien
openaire   +3 more sources

Stabilization of F-Actin Cytoskeleton by Paclitaxel Improves the Blastocyst Developmental Competence through P38 MAPK Activity in Porcine Embryos

open access: yesBiomedicines, 2022
Changes in F-actin distribution and cortical F-actin morphology are important for blastocyst developmental competence during embryogenesis. However, the effect of paclitaxel as a microtubule stabilizer on embryonic development in pigs remains unclear. We
Seung-Yeon Joe   +4 more
doaj   +1 more source

The COOH terminus of the c-Abl tyrosine kinase contains distinct F- and G-actin binding domains with bundling activity [PDF]

open access: yes, 1994
The myristoylated form of c-Abl protein, as well as the P210bcr/abl protein, have been shown by indirect immunofluorescence to associate with F-actin stress fibers in fibroblasts. Analysis of deletion mutants of c-Abl stably expressed in fibroblasts maps
Baltimore, David   +5 more
core   +4 more sources

In vivo imaging of cell behaviors and F-actin reveals LIM-HD transcription factor regulation of peripheral versus central sensory axon development [PDF]

open access: yes, 2011
Background Development of specific neuronal morphology requires precise control over cell motility processes, including axon formation, outgrowth and branching.
Erica F Andersen   +2 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

Induced Arp2/3 Complex Depletion Increases FMNL2/3 Formin Expression and Filopodia Formation

open access: yesFrontiers in Cell and Developmental Biology, 2021
The Arp2/3 complex generates branched actin filament networks operating in cell edge protrusion and vesicle trafficking. Here we employ a conditional knockout mouse model permitting tissue- or cell-type specific deletion of the murine Actr3 gene ...
Vanessa Dimchev   +14 more
doaj   +1 more source

Stress relaxation in F-actin solutions by severing

open access: yes, 2019
Networks of filamentous actin (F-actin) are important for the mechanics of most animal cells. These cytoskeletal networks are highly dynamic, with a variety of actin-associated proteins that control cross-linking, polymerization and force generation in ...
Arzash, S.   +4 more
core   +1 more source

Cytoskeletal stabilization of inhibitory interactions in immunologic synapses of mature human dendritic cells with natural killer cells [PDF]

open access: yes, 2011
Human mature dendritic cells (DCs) can efficiently stimulate natural killer (NK)-cell responses without being targeted by their cytotoxicity. To understand this important regulatory crosstalk, we characterized the development of the immunologic synapse ...
Barreira da Silva, Rosa   +2 more
core   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

open access: yesFEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto   +3 more
wiley   +1 more source

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