Results 51 to 60 of about 672,429 (301)

Targeting F-actin stress fibers to suppress the dedifferentiated phenotype in chondrocytes

European Journal of Cell Biology
Actin is a central mediator of the chondrocyte phenotype. Monolayer expansion of articular chondrocytes on tissue culture polystyrene, for cell-based repair therapies, leads to chondrocyte dedifferentiation.
Mandy M. Schofield, Alissa T. Rzepski, Stephanie Richardson-Solorzano, Jonah Hammerstedt, Sohan Shah, Chloe E. Mirack, Marin Herrick, Justin Parreno   +7 more
doaj   +1 more source

Origin and arrangement of actin filaments for gliding motility in apicomplexan parasites revealed by cryo-electron tomography

Nature Communications, 2023
The phylum Apicomplexa comprises important eukaryotic parasites that invade host tissues and cells using a unique mechanism of gliding motility. Gliding is powered by actomyosin motors that translocate host-attached surface adhesins along the parasite ...
Matthew Martinez, Shrawan Kumar Mageswaran, Amandine Guérin, William David Chen, Cameron Parker Thompson, Sabine Chavin, Dominique Soldati-Favre, Boris Striepen, Yi-Wei Chang   +8 more
doaj   +1 more source

Tracer Studies on F-Actin Fluctuations [PDF]

Physical Review Letters, 2002
We present a study on the fluctuations of semiflexible actin filaments using fluorescence videomicroscopy, focusing on the end-to-end fluctuations of single filaments. In order to specifically measure the position of the polymer's ends, we developed a novel noninvasive method that consists of annealing short end tags to the filaments. This allows us to
Le Goff, Loïc, Hallatschek, Oskar, Frey, Erwin, Amblard, François   +3 more
openaire   +5 more sources

Roles of Three Domains of Tetrahymena eEF1Ain Bundling F-actin [PDF]

, 2008
The conventional role of eukaryotic elongation factor 1A (eEF1A) is to transport aminoacyl tRNA to the A site of ribosomes during the peptide elongation phase of protein synthesis.
Bunai Fumihide, Morita Kenya, Numata Osamu, 沼田 治   +3 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

F-Actin Dynamics in Neurospora crassa [PDF]

Eukaryotic Cell, 2010
ABSTRACT This study demonstrates the utility of Lifeact for the investigation of actin dynamics in Neurospora crassa and also represents the first report of simultaneous live-cell imaging of the actin and microtubule cytoskeletons in filamentous fungi.
Berepiki, Adokiye, Lichius, Alexander, Shoji, Jun Y., Tilsner, Jens, Read, Nick D.   +4 more
openaire   +4 more sources

Filamin cross-linked semiflexible networks: Fragility under strain

, 2006
The semiflexible F-actin network of the cytoskeleton is cross-linked by a variety of proteins including filamin, which contain Ig-domains that unfold under applied tension.
Alex J. Levine, B. A. DiDonna, B. Alberts, E. Evans, E. Frey, E. L. Elson, O. Kallmes, R. Satcher   +7 more
core   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

FEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure, Misaki K. Nishio, Satoru Okuda, Kohji Hotta   +3 more
wiley   +1 more source

Real-time measurement of F-actin remodelling during exocytosis using Lifeact-EGFP transgenic animals.

PLoS ONE, 2012
F-actin remodelling is essential for a wide variety of cell processes. It is important in exocytosis, where F-actin coats fusing exocytic granules. The purpose of these F-actin coats is unknown.
Yujin Jang, Carolina Soekmadji, Justin M Mitchell, Walter G Thomas, Peter Thorn   +4 more
doaj   +1 more source

Coactosin-like 1 antagonizes cofilin to promote lamellipodial protrusion at the immune synapse. [PDF]

PLoS ONE, 2014
Actin depolymerizing factor-homology (ADF-H) family proteins regulate actin filament dynamics at multiple cellular locations. Herein, we have investigated the function of the ADF-H family member coactosin-like 1 (COTL1) in the regulation of actin ...
Joanna Kim, Michael J Shapiro, Adebowale O Bamidele, Pinar Gurel, Puspa Thapa, Henry N Higgs, Karen E Hedin, Virginia S Shapiro, Daniel D Billadeau   +8 more
doaj   +1 more source