Results 21 to 30 of about 4,584 (262)
Ankyrin is fatty acid acylated in erythrocytes. [PDF]
Ankyrin is a peripheral membrane protein that mediates the attachment of the erythrocyte membrane skeleton to the plasma membrane. We show that [3H]palmitic acid is incorporated into ankyrin in vivo. The majority of the 3H-labeled fatty acid is covalently bound to the polypeptide, as it cannot be removed by strong detergents or by chloroform/methanol ...
Staufenbiel, Matthias, Lazarides, Elias
openaire +4 more sources
A comprehensive classification system for lipids1
Lipids are produced, transported, and recognized by the concerted actions of numerous enzymes, binding proteins, and receptors. A comprehensive analysis of lipid molecules, “lipidomics,” in the context of genomics and proteomics is crucial to ...
Eoin Fahy +16 more
doaj +1 more source
Lipidomic profiling of the developing kernel clarifies the lipid metabolism of Paeonia ostii
Lipid components in the developing kernel of Paeonia ostii were determined, and the fatty acid (FA) distributions in triacylglycerol and phospholipids were characterized. The lipids in the kernel were mainly phospholipids (43%), neutral glycerides (24%),
Shui-Yan Yu +4 more
doaj +1 more source
In animals, myostatin (MSTN) is a negative regulator that inhibits muscle growth and repair. The decreased level of functional MSTN gene expression can change the amount and proportions of fats in pigs.
Yangli Pei +7 more
doaj +1 more source
The various biosynthetic modifications of proteins, as for instance the addition of oligosaccharides, phosphate groups and fatty acid chains have become an important area of study in the biomedical sciences. Especially in a time where cloned eukaryotic genes are expressed in bacterial (or other alien) systems yielding non-modified protein products for ...
openaire +2 more sources
Dynamic fatty acylation of p21N‐ras. [PDF]
To study the acylation of p21N-ras with palmitic acid we have used cells which express the human N-ras gene to high levels under control of the steroid-inducible MMTV--LTR promoter. Addition of [3H]palmitate to these cells resulted in detectable incorporation of label into p21N-ras within 5 min, which continued linearly for 30-60 min.
A I, Magee +4 more
openaire +2 more sources
Multiple isozymes are encoded in the Caenorhabditis elegans genome for the various sphingolipid biosynthesis reactions, but the contributions of individual isozymes are characterized only in part. We developed a simple but effective reversed-phase liquid
Hui Luo +6 more
doaj +1 more source
Effect of chitosan on buck semen quality and semen plasma metabolites during low-temperature storage
BackgroundOptimizing buck semen preservation techniques can significantly advance the goat industry. This study aimed to investigate the effects of chitosan on sperm quality and seminal plasma metabolite profiles in bucks during low-temperature storage ...
Meijun Song +21 more
doaj +1 more source
Proteomic analysis of fatty-acylated proteins [PDF]
Protein fatty-acylation in eukaryotes has been associated with many fundamental biological processes. However, the diversity, abundance and regulatory mechanisms of protein fatty-acylation in vivo remain to be explored. Herein, we review the proteomic analysis of fatty-acylated proteins, with a focus on N-myristoylation and S-palmitoylation.
Peng, Tao +2 more
openaire +2 more sources
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source

