A practical, biomimetic, one-pot synthesis of firefly luciferin [PDF]
Scientific ReportsThe bioluminescence reaction of firefly luciferase with D-luciferin has become an indispensable imaging technique in modern biology and life science experiments, but the high cost of D-luciferin is limiting its further application.
Maria Kato +4 more
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Biosynthesis of firefly luciferin in adult lantern: decarboxylation of L-cysteine is a key step for benzothiazole ring formation in firefly luciferin synthesis. [PDF]
PLoS ONE, 2013 Bioluminescence in fireflies and click beetles is produced by a luciferase-luciferin reaction. The luminescence property and protein structure of firefly luciferase have been investigated, and its cDNA has been used for various assay systems.
Yuichi Oba +4 more
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Biosensing firefly luciferin synthesis in bacteria reveals a cysteine-dependent quinone detoxification route in Coleoptera [PDF]
Scientific Reports, 2022 Luciferin biosynthetic origin and alternative biological functions during the evolution of beetles remain unknown. We have set up a bioluminescent sensing method for luciferin synthesis from cysteine and benzoquinone using E.
Daniel Rangel de Souza +3 more
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How to Select Firefly Luciferin Analogues for In Vivo Imaging. [PDF]
Int J Mol Sci, 2021 Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models.
Saito-Moriya R +6 more
europepmc +4 more sources
Triple Bioluminescence Imaging for In Vivo Monitoring of Cellular Processes [PDF]
Molecular Therapy: Nucleic Acids, 2013 Bioluminescence imaging (BLI) has shown to be crucial for monitoring in vivo biological processes. So far, only dual bioluminescence imaging using firefly (Fluc) and Renilla or Gaussia (Gluc) luciferase has been achieved due to the lack of availability ...
Casey A Maguire +8 more
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Luciferase expression and bioluminescence does not affect tumor cell growth
Molecular Cancer, 2010 Live animal imaging is becoming an increasingly common technique for accurate and quantitative assessment of tumor burden over time. Bioluminescence imaging systems rely on a bioluminescent signal from tumor cells, typically generated from expression of ...
Rasko John EJ +4 more
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Tracking adipose-derived mesenchymal stromal cells in the eye: Integrating IVIS imaging and Alu PCR for enhanced detection of human cells [PDF]
Regenerative TherapyIntroduction: Cell transplantation finds broad applications in medical science, with applications ranging from stem cell therapies to cancer research. Despite its widespread use, inherent risks such as tumor formation and immune rejection necessitate a ...
Robert M. Rusch +8 more
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Correction: Biosynthesis of Firefly Luciferin in Adult Lantern: Decarboxylation of L-Cysteine is a Key Step for Benzothiazole Ring Formation in Firefly Luciferin Synthesis [PDF]
PLoS ONE, 2014 doaj +4 more sources
A Microplate-Based Expression Monitoring System for Arabidopsis NITRATE TRANSPORTER2.1 Using the Luciferase Reporter [PDF]
Bio-ProtocolGene expression analysis is a fundamental technique to elucidate the regulatory mechanisms of genes of interest or to reveal the patterns of plant response to environmental stimuli.
Yoshiaki Ueda, Shuichi Yanagisawa
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Transcriptome analysis reveals candidate genes involved in luciferin metabolism in Luciola aquatilis (Coleoptera: Lampyridae) [PDF]
PeerJ, 2016 Bioluminescence, which living organisms such as fireflies emit light, has been studied extensively for over half a century. This intriguing reaction, having its origins in nature where glowing insects can signal things such as attraction or defense, is ...
Wanwipa Vongsangnak +2 more
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