Results 11 to 20 of about 20,810 (248)

Immobilization of Firefly Bioluminescent System: Development and Application of Reagents

Biosensors, 2022
The present study describes the method of preparing reagents containing firefly luciferase (FLuc) and its substrate, D-luciferin, immobilized into gelatin gel separately or together.
Elena N. Esimbekova, Maria A. Kirillova, Valentina A. Kratasyuk   +2 more
doaj   +1 more source

Mathematical Model of the Firefly Luciferase Complementation Assay Reveals a Non-Linear Relationship between the Detected Luminescence and the Affinity of the Protein Pair Being Analyzed. [PDF]

PLoS ONE, 2016
The firefly luciferase complementation assay is widely used as a bioluminescent reporter technology to detect protein-protein interactions in vitro, in cellulo, and in vivo.
Renee Dale, Yuki Ohmuro-Matsuyama, Hiroshi Ueda, Naohiro Kato   +3 more
doaj   +1 more source

A Novel Brighter Bioluminescent Fusion Protein Based on ZZ Domain and Amydetes vivianii Firefly Luciferase for Immunoassays

Frontiers in Bioengineering and Biotechnology, 2021
Immunoassays are widely used for detection of antibodies against specific antigens in diagnosis, as well as in electrophoretic techniques such as Western Blotting.
Vadim R. Viviani, Vadim R. Viviani, Jaqueline Rodrigues Silva, Jaqueline Rodrigues Silva, Paulo Lee Ho   +4 more
doaj   +1 more source

Improving the Stability of Protein–Protein Interaction Assay FlimPIA Using a Thermostabilized Firefly Luciferase

Frontiers in Bioengineering and Biotechnology, 2021
The protein–protein interaction assay is a key technology in various fields, being applicable in drug screening as well as in diagnosis and inspection, wherein the stability of assays is important.
Yuki Ohmuro-Matsuyama, Yuki Ohmuro-Matsuyama, Yuki Ohmuro-Matsuyama, Keiko Gomi, Takuya Shimoda, Hideki Yamaji, Hiroshi Ueda   +6 more
doaj   +1 more source

5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems.

PLoS ONE, 2020
Firefly luciferase-based ATP detection assays are frequently used as a sensitive, cost-efficient method for monitoring hygiene in many industrial settings.
Ce Shi, Michael P Killoran, Mary P Hall, Paul Otto, Monika G Wood, Ethan Strauss, Lance P Encell, Thomas Machleidt, Keith V Wood, Thomas A Kirkland   +9 more
doaj   +1 more source

A QM/MM Study on the Initiation Reaction of Firefly Bioluminescence—Enzymatic Oxidation of Luciferin

Molecules, 2021
Among all bioluminescent organisms, the firefly is the most famous, with a high luminescent efficiency of 41%, which is widely used in the fields of biotechnology, biomedicine and so on.
Mohan Yu, Yajun Liu
doaj   +1 more source

Adeno‐associated virus serotype 2 capsid variants for improved liver‐directed gene therapy

Hepatology, EarlyView., 2022
Abstract Background and Aims Current liver‐directed gene therapies look for adeno‐associated virus (AAV) vectors with improved efficacy. With this background, capsid engineering is explored. Whereas shuffled capsid library screenings have resulted in potent liver targeting variants with one first vector in human clinical trials, modifying natural ...
Nadja Meumann, Marti Cabanes‐Creus, Moritz Ertelt, Renina Gale Navarro, Julie Lucifora, Qinggong Yuan, Karin Nien‐Huber, Ahmed Abdelrahman, Xuan‐Khang Vu, Liang Zhang, Ann‐Christin Franke, Christian Schmithals, Albrecht Piiper, Annabelle Vogt, Maria Gonzalez‐Carmona, Jochen T. Frueh, Evelyn Ullrich, Philip Meuleman, Steven R. Talbot, Margarete Odenthal, Michael Ott, Erhard Seifried, Clara T. Schoeder, Joachim Schwäble, Leszek Lisowski, Hildegard Büning   +25 more
wiley   +1 more source

The location and nature of general anesthetic binding sites on the active conformation of firefly luciferase; a time resolved photolabeling study. [PDF]

PLoS ONE, 2012
Firefly luciferase is one of the few soluble proteins that is acted upon by a wide variety of general anesthetics and alcohols; they inhibit the ATP-driven production of light.
Sivananthaperumal Shanmugasundararaj, Simon Lehle, Herve I Yamodo, S Shaukat Husain, Claire Tseng, Khanh Nguyen, George H Addona, Keith W Miller   +7 more
doaj   +1 more source

Genomic and experimental data provide new insights into luciferin biosynthesis and bioluminescence evolution in fireflies

Scientific Reports, 2020
Fireflies are among the most charismatic insects for their spectacular bioluminescence, but the origin and evolution of bioluminescence remain elusive.
Ru Zhang, Jinwu He, Zhiwei Dong, Guichun Liu, Yuan Yin, Xinying Zhang, Qi Li, Yandong Ren, Yongzhi Yang, Wei Liu, Xianqing Chen, Wenhao Xia, Kang Duan, Fei Hao, Zeshan Lin, Jie Yang, Zhou Chang, Ruoping Zhao, Wenting Wan, Sihan Lu, Yanqiong Peng, Siqin Ge, Wen Wang, Xueyan Li   +23 more
doaj   +1 more source

The HSV-1 Latency-Associated Transcript Functions to Repress Latent Phase Lytic Gene Expression and Suppress Virus Reactivation from Latently Infected Neurons [PDF]

, 2016
open access articleHerpes simplex virus 1 (HSV-1) establishes life-long latent infection within sensory neurons, during which viral lytic gene expression is silenced.
Coleman, Heather M., Connor, Viv, Efstathiou, Stacey, Hann, William, Harman, Laura E.R., Nicoll, Michael P., Proenca, Joao T., Shivkumar, Maitreyi   +7 more
core   +3 more sources