Using bioluminescence to image gene expression and spontaneous behavior in freely moving mice.
PLoS ONE, 2023 Bioluminescence imaging (BLI) of gene expression in live animals is a powerful method for monitoring development, tumor growth, infections, healing, and other progressive, long-term biological processes. BLI remains an effective approach for reducing the
Astha Malik +2 more
doaj +1 more source
Molecular Therapy: Oncolytics, 2021 Bioluminescence reporter gene imaging is a robust, high-throughput imaging modality that is useful for tracking cells and monitoring biological processes, both in cell culture and in small animals.
Maxim A. Moroz +10 more
doaj +1 more source
Scientific Reports, 2018 The New Zealand glowworm, Arachnocampa luminosa, is well-known for displays of blue-green bioluminescence, but details of its bioluminescent chemistry have been elusive.
Oliver C. Watkins +3 more
doaj +1 more source
Optogenetic inhibition of neurons by internal light production
Frontiers in Behavioral Neuroscience, 2014 Optogenetics is an extremely powerful tool for selective neuronal activation/inhibition and dissection of neural circuits. However, a limitation of in vivo optogenetics is that an animal must be tethered to an optical fiber for delivery of light.
Benjamin eLand +4 more
doaj +1 more source
A photocytes-associated fatty acid-binding protein from the light organ of adult Taiwanese firefly, Luciola cerata. [PDF]
PLoS ONE, 2011 BACKGROUND: Intracellular fatty acid-binding proteins (FABPs) are considered to be an important energy source supplier in lipid metabolism; however, they have never been reported in any bioluminescent tissue before.
King-Siang Goh, Chia-Wei Li
doaj +1 more source
Basement membrane-rich Organoids with functional human blood vessels are permissive niches for human breast cancer metastasis [PDF]
, 2013 Metastasic breast cancer is the leading cause of death by malignancy in women worldwide. Tumor metastasis is a multistep process encompassing local invasion of cancer cells at primary tumor site, intravasation into the blood vessel, survival in systemic ...
Alonso-Camino, Vanesa +12 more
core +7 more sources
Influence of Bioluminescence Imaging Dynamics by D-Luciferin Uptake and Efflux Mechanisms
Molecular Imaging, 2012 Bioluminescence imaging (BLI) detects light generated by luciferase-mediated oxidation of substrate and is used widely for evaluating transgene expression in cell-based assays and in vivo in relevant preclinical models.
Yimao Zhang +3 more
doaj +1 more source
Decrease of catalytic efficiency of Photinus pyralis firefly luciferase in the presence of graphene quantum dots [PDF]
Nanomedicine Journal, 2020 Objective(s): Firefly luciferase is a monooxygenase enzyme that emits flash of light during the enzymatic reaction. Luciferase has been used in many bioanalytical fields from ATP detection methods to in vivo imaging.
Elaheh Samadi +3 more
doaj +1 more source
Insights into the kinetics of siRNA-mediated gene silencing from live-cell and live-animal bioluminescent imaging [PDF]
, 2006 Small interfering RNA (siRNA) molecules are potent effectors of post-transcriptional gene silencing. Using noninvasive bioluminescent imaging and a mathematical model of siRNA delivery and function, the effects of target-specific and treatment-specific ...
Bartlett, Derek W., Davis, Mark E.
core +1 more source
Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging [PDF]
, 2018 The sensitivity of bioluminescence imaging in animals is primarily dependent on the amount of photons emitted by the luciferase enzyme at wavelengths greater than 620 nm where tissue penetration is high.
Encell, L.P. (Lance P.) +11 more
core +1 more source