Results 71 to 80 of about 82,939 (224)

D‐amino acid oxidase as a chemogenetic tool for spatiotemporally controlled hydrogen peroxide production: The oxygen connection

British Journal of Pharmacology, EarlyView.
Abstract Background and Purpose D‐amino acid oxidase (DAO) has been developed as a chemogenetic tool, to precisely manipulate redox levels in tissues and cells by generating H2O2 in the presence of D‐amino acids. The enzyme's significant oxygen consumption during H2O2 production may influence cellular oxygen levels.
Wiebke Maurer, Anke Zieseniss, Vamsi Priya Vikram, Yamin Chen, Laura Bauer, Annette Hillemann, Peter Rehling, Maithily S. Nanadikar, Diane de Zélicourt, Vartan Kurtcuoglu, Dörthe M. Katschinski   +10 more
wiley   +1 more source

Selective fluorescent detection of flavin adenine dinucleotide in human eosinophils by using bis(Zn2+-dipicolylamine) complex

, 2015
A novel fluorescent sensor based on the Zn2+-DPA complex shows selective detection of flavin adenine dinucleotide (FAD) from among other flavins in water, and it was used to detect FAD in eosinophils by fluorescence microscopy and ...
Han, Kyungja, Rhee, Hyun-Woo, Choi, Ha-Young, Hong, Jong-In   +3 more
core   +1 more source

Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues [PDF]

Journal of Innovative Optical Health Sciences, 2019
The endogenous fluorophores such as reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) in cells and tissues can be imaged by fluorescence lifetime imaging microscopy (FLIM) to show the tissue morphology ...
Maojia Huang, Zixiao Zhang, Xinyi Wang, Yonghui Xie, Yiyan Fei, Jiong Ma, Jing Wang, Li Chen, Lan Mi, Yulan Wang   +9 more
doaj   +1 more source

Non‐canonical PKG1 regulation in cardiovascular health and disease

British Journal of Pharmacology, EarlyView.
It is well established that the cyclic GMP‐dependent protein kinase I (PKG1) is canonically activated by cyclic guanosine monophosphate (cGMP), enabling its regulation of vascular tone, cardiac function and smooth muscle homeostasis. However, diverse non‐canonical stimuli of PKG1 have also been identified.
Jie Su, Joseph Robert Burgoyne
wiley   +1 more source

Synthesis of phosphodiester-type nicotinamide adenine dinucleotide analogs

, 2009
Fourteen phosphodiester-type beta-nicotinamide adenine dinucleotide (NAD+) analogs were prepared starting from nicotinamide. The phosphodiester linkage was effectively assembled in 69-93% yields via condensation reaction between 2'.3'-di-O-acetyl ...
Wu, Siguo, 赵宗保, Hou, Shuhua, Zhao, Zongbao (Kent), Liu, Wujun   +4 more
core   +1 more source

Not Cleaving the His-tag of Thal Results in More Tightly Packed and Better-Diffracting Crystals

Crystals, 2020
Flavin-dependent halogenases chlorinate or brominate their substrates in an environmentally friendly manner, only requiring the cofactor reduced flavin adenine dinucleotide (FADH2), oxygen, and halide salts.
Ann-Christin Moritzer, Tina Prior, Hartmut H. Niemann   +2 more
doaj   +1 more source

Studies on the Dissociation of Flavin Adenine Dinucleotide from Metalloflavoproteins

Journal of Biological Chemistry, 1972
Abstract Treatment of milk xanthine oxidase and chicken liver xanthine dehydrogenase with high concentrations of salts for several hours resulted in the loss of reactivity toward O2 and NAD, respectively. Oxidation of xanthine by either enzyme in the presence of electron acceptors such as dichlorophenol indophenol was unimpaired under these conditions,
M, Kanda, F O, Brady, K V, Rajagopalan, P, Handler   +3 more
openaire   +2 more sources

A chemo‐enzymatic colorimetric cascade for detecting ethylene glycol contamination

The FEBS Journal, EarlyView.
A chemo‐enzymatic cascade is developed for ethylene glycol (EG) detection. EG is oxidized by GoADH with C1‐mediated cofactor recycling to produce glycolaldehyde (GAL). GAL subsequently reacts with purpald to generate a detectable purple chromophore.
Tawatchai Singphongam, Tadtarit Anujareewat, Wachirawit Chinantuya, Pratchaya Watthaisong, Chayasith Uttamapinant, Somchart Maenpuen, Ruchanok Tinikul   +6 more
wiley   +1 more source

Dynamic conformations of flavin adenine dinucleotide: Simulated molecular dynamics of the flavin cofactor related to the time-resolved fluorescence characteristics

, 2002
Molecular dynamics (MD) simulations and polarized subnanosecond time-resolved flavin fluorescence spectroscopy have been used to study the conformational dynamics of the flavin adenine dinucleotide (FAD) cofactor in aqueous solution.
K. Anton Feenstra, Berendsen, H. J. C., Feenstra, K. A., Berendsen, H.J.C., Visser, AJWG, Herman J. C. Berendsen, Visser, A. J. W. G., Mark, A.E., van den Berg, P.A.W., Mark, A. E., Feenstra, K.A., Feenstra, K.A, Petra A. W. van den Berg, Berg, P.A.W., van den, Alan E. Mark, Antonie J. W. G. Visser, Feenstra, K.A.; id_orcid, Visser, A.J.W.G., van den Berg, P. A. W.   +18 more
core   +1 more source

Characterization of Two VAO-Type Flavoprotein Oxidases from Myceliophthora thermophila

Molecules, 2018
The VAO flavoprotein family consists mostly of oxidoreductases harboring a covalently linked flavin cofactor. The linkage can be either monocovalent at position 8 with a histidine or tyrosine or bicovalent at position 8 with a histidine and at position 6
Alessandro R. Ferrari, Henriëtte J. Rozeboom, Aniek S. C. Vugts, Martijn J. Koetsier, Robert Floor, Marco W. Fraaije   +5 more
doaj   +1 more source