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Deconvolution of fluorescence lifetime imaging microscopy (FLIM) [PDF]
Multiphoton Microscopy in the Biomedical Sciences XXII, 2022 Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two fluorophores within a diffraction limit are excited, the resulting emission leads to non-linear spatial distortion and ...
Varun Mannam +2 more
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Fluorescence Lifetime Imaging Microscopy (FLIM): Instrumentation and Applications
Critical Reviews in Analytical Chemistry, 1992Abstract The new and novel techniques of fluorescence lifetime imaging (FLI)∗∗ and fluorescence lifetime imaging microscopy (FLIM) provide the investigator with the capacity to quantitate two-dimensional fluorescence intensity distributions and lifetimes. The concept, theory, and instrumentation of FLI and FLIM are reviewed in this paper.
Ammasi Periasamy +2 more
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Imaging Vacuolar Anthocyanins with Fluorescence Lifetime Microscopy (FLIM)
Methods in Molecular Biology, 2018Anthocyanins are intrinsically fluorescent pigments that accumulate in plant vacuoles. We have developed a platform to analyze the fluorescence decay of anthocyanins by fluorescence lifetime imaging microscopy (FLIM), under in vitro and in vivo conditions. Fluorescence lifetime of a fluorophore can be influenced by temperature, pH, oxygen concentration,
Kevin Eliceiri +2 more
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Fluorescence Lifetime Imaging Microscopy (FLIM)
2005Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems have been implemented both in the frequency domain, using sinusoidally intensity-modulated excitation light and modulated detectors, and in the time domain, using pulsed excitation ...
Erik B, van Munster +1 more
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Applying image restoration to fluorescence lifetime imaging microscopy (FLIM)
SPIE Proceedings, 2009We describe a novel approach using 2D-intensity-deconvolution to improve spatial resolution in wide-field FLIM. The method maintains lifetime accuracy and can restore features within experimentally reasonable intensity ranges.
Ching-Wei Chang, Mary-Ann Mycek
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Fluorescence lifetime imaging (FLIM) of rhodamine 123 in living cells
Photochemical & Photobiological Sciences, 2004A novel setup for fluorescence intensity and lifetime imaging (FLIM) of living cells is reported. Time-resolving techniques are combined with total internal reflection fluorescence microscopy (TIRFM), which permits optical excitation of either plasma membranes or whole cells depending on whether the angle of incidence of the excitation light is greater
Herbert, Schneckenburger +4 more
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Studying Membrane Properties Using Fluorescence Lifetime Imaging Microscopy (FLIM)
Springer Series on Fluorescence, 2012Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool to investigate the structure and composition of biological membranes. A wide variety of fluorescent probes suitable for FLIM experiments have been described. These compounds differ strongly in the details of their incorporation into membranes and in their responses toward changes in the
Vinod Subramaniam +2 more
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Application of fluorescence lifetime imaging (FLIM) in latent finger mark detection
Forensic Science International, 2006Fluorescence lifetime imaging (FLIM) in frequency domain enables the mapping of the spatial distribution of fluorescence lifetimes of a specimen. It has been extensively applied in biology. In this paper, a theoretical analysis for the fluorescence lifetime determination of latent finger mark samples is described, which is followed by the feasibility ...
Vadakke M Murukeshan, L K Seah, L K Seah
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Fluorescence Lifetime Imaging (FLIM) Measurements in Salinity Research
2012Plant response to salt stress involves changes in intracellular ion concentrations that can be estimated by ion-selective fluorescent dyes. Conventional confocal/fluorescent imaging does not always accurately reflect the process, since it is dependent not only on the ion concentration but on the dye concentration itself.
Olga, Babourina, Zed, Rengel
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