Results 151 to 160 of about 6,539 (187)

mb-FLIM: Model-based fluorescence lifetime imaging

SPIE Proceedings, 2012
We have developed a model-based, parallel procedure to estimate fluorescence lifetimes. Multiple frequencies are present in the excitation signal. Modeling the entire fluorescence and measurement process produces an analytical ratio of polynomials in the lifetime variable τ. A non-linear model-fitting procedure is then used to estimate τ.
Qiaole Zhao, Ian Ted Young, Raymond Schouten, Sjoerd Stallinga, Kees Jalink, Sander de Jong   +5 more
openaire   +1 more source

Fluorescence lifetime imaging microscopy (FLIM): Spatial resolution of microstructures on the nanosecond time scale

Biophysical Chemistry, 1993
A frequency domain fluorescence lifetime imaging microscope (FLIM) has been developed. A continuous wave laser excitation source of an epi-illumination fluorescence microscope is modulated at a high frequency fk The lifetime of the modulated fluorescence emission is determined from the phase delay and modulation depth of the fluorescence signal ...
Thomas M Jovin, Theodorus W J Gadella, Robert M Clegg   +2 more
exaly   +5 more sources

Fluorescence lifetime imaging microscopy (FLIM) on the single molecule level

Laser Applications to Chemical and Environmental Analysis, 2001
During the last years, several methods for the detection of individual fluorescently labeled molecules have been developed.
Phillip Tinnefeld, V. Buschmann, D.-P. Herten, J.-P. Knemeyer, M. Sauer   +4 more
openaire   +1 more source

Fluorescence lifetime imaging (FLIM) of membrane markers in living cells

Confocal, Multiphoton, and Nonlinear Microscopic Imaging, 2003
An experimental setup for fluorescence lifetime imaging (FLIM) has been combined with total internal reflection fluorescence microscopy (TIRFM) in order to detect various membrane markers within living cells. The method is established using T47D human breast cancer cells transfected by a plasmid encoding for a membrane associated yellow fluorescent ...
Herbert Schneckenburger, Michael Wagner, Martina Kretzschmar, Wolfgang S. Strauss, Reinhard Sailer   +4 more
openaire   +1 more source

Fluorescence and fluorescence-lifetime imaging microscopy (FLIM) to characterize yeast strains by autofluorescence

SPIE Proceedings, 2006
We characterised populations of wild type baking and brewing yeast cells using intrinsic fluorescence and fluorescence lifetime microscopy, in order to obtain quantitative identifiers of different strains. The cell autofluorescence was excited at 405 nm and observed within 440-540 nm range where strong cell to cell variability was observed.
H. Bhatta, E. M. Goldys, J. Ma
openaire   +1 more source

Fluorescence Lifetime Imaging (FLIM): Basic Concepts and Recent Applications

2015
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photo physical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is independent of the local fluorophore concentration and excitation intensity. A
Suhling, Klaus, Hirvonen, Liisa, Levitt, James, Chung, Pei-Hua, Tregido, Carolyn, Le Marois, Alix, Rusakov, Dmitri A., Zheng, Kaiyu, Ameer-Beg, Simon, Poland, Simon, Coelho, Simon, Dimble, Richard, Hirvonen, Liisa Maija   +12 more
openaire   +2 more sources

Analysis of Fluorescence Lifetime Imaging Microscopy (FLIM) Data

Methods of Information in Medicine, 2010
Summary Objectives: A novel Fluorescence Lifetime Imaging Microscopy (FLIM) deconvolution method based on the linear expansion of fluorescence decays on a set of orthonormal Laguerre functions was recently proposed. The Laguerre deconvolution method applies linear least-square estimation to estimate the expansion coefficients of all pixel ...
P, Pande, C A, Trivedi, J A, Jo
openaire   +2 more sources

Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments

Medical Photonics, 2015
Summary Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is independent of the local fluorophore concentration and excitation ...
Suhling, Klaus, Hirvonen, Liisa Maija, Levitt, James Alan, Chung, Pei-Hua, Tregidgo, Carolyn Lisa, Le Marois, Alix Marie, Rusakov, Dmitri A., Zheng, Kaiyu, Ameer-Beg, Simon, Poland, Simon Peter, Pereira Coelho, Simao Pedro, Henderson, Robert, Krstajić, Nikola   +12 more
openaire   +2 more sources

Fluorescence Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM)

2006
Current approaches and developments in the field of microscopy based on determinations of Fluorescence (Forster) Resonance Energy Transfer (FRET) and Fluorescence Lifetime (FLIM) are discussed.
Jovin, T., Lidke, D., Jares-Erijman, E.
openaire   +2 more sources

Confocal Fluorescence Lifetime Imaging Microscopy (FLIM) at the Single Molecule Level

Single Molecules, 2000
We report on confocal fluorescence lifetime imaging microscopy (CFLIM) of single dye molecules adsorbed on glass surface. Applying a short-pulse diode laser emitting at 635 nm with a repetition rate of 64 MHz we studied the time-resolved identification of individual carbocyanine and oxazine dyes via their characteristic fluorescence lifetimes of 2.06±0.
Philip Tinnefeld, Volker Buschmann, Dirk-Peter Herten, Kyung-Tae Han, Markus Sauer   +4 more
openaire   +1 more source