Results 161 to 170 of about 6,539 (187)

Application of Fluorescence Lifetime Imaging (FLIM) to Measure Intracellular Environments in a Single Cell

2017
Fluorescence lifetime imaging (FLIM) has now been used in many bioscience fields, which comes from the quantification of fluorescence lifetime. The procedure for obtaining lifetime images is very similar to that used in fluorescence microscopy. However, obtaining reliable lifetime images requires an understanding of the theory of fluorescence lifetime,
Takakazu, Nakabayashi, Kamlesh, Awasthi, Nobuhiro, Ohta   +2 more
openaire   +2 more sources

Parallelized fluorescence lifetime imaging microscopy (FLIM) based on photon reassignment

Optics Communications, 2018
Abstract We propose a novel parallelized fluorescence lifetime imaging microscopy (FLIM) method based on photon reassignment with a detector array and time-correlated single photon counting (TCSPC) array. The detector array composed of seven avalanche photon diodes (APDs) substitutes for original pinhole and detector to constitute an imaging scanning
Shaocong Liu, Zhimin Zhang, Jiaoyang Zheng, Liang Xu, Cuifang Kuang, Xu Liu   +5 more
openaire   +1 more source

Quantification of Astrocytic Sodium Signals Using Fluorescence Lifetime Imaging Microscopy (FLIM)

Intensity-based imaging with fluorescent indicators is a widely used method for monitoring ion transients, including Ca2+ or Na+ signals, in astrocytes. However, using this technique, changes in ion concentrations cannot always be reliably separated from simultaneous changes in the concentration of the ion-sensitive fluorophores, e.g., due to changes ...
Jan, Meyer, Karl W, Kafitz, Christine R, Rose   +2 more
openaire   +2 more sources

New application for the identification and differentiation of microplastics based on fluorescence lifetime imaging microscopy (FLIM)

Journal of Environmental Chemical Engineering, 2021
Heinz Langhals, Daniel Dietrich
exaly  

Label-free identification and differentiation of different microplastics using phasor analysis of fluorescence lifetime imaging microscopy (FLIM)-generated data

Chemico-Biological Interactions, 2021
Weronika Schary, Heinz Langhals, Daniel Dietrich   +2 more
exaly  

Light-field tomographic fluorescence lifetime imaging microscopy (LIFT-FLIM)

Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences
Fluorescence lifetime imaging microscopy (FLIM) measures fluorescence lifetimes of fluorescent probes to investigate molecular interactions. However, conventional FLIM systems often requires extensive scanning that is time-consuming. To address this challenge, we developed a novel computational imaging technique called light field tomographic FLIM ...
Ma, Yayao, Huang, Luzhe, Sen, Chandani, Burri, Samuel, Bruschini, Claudio, Yang, Xilin, Cameron, Robert B., Fishbein, Gregory A., Gomperts, Brigitte N., Özcan, Aydogan, Charbon, Edoardo, Gao, Liang   +11 more
openaire   +1 more source

Fluorescence lifetime imaging microscopy (FLIM) detects differences in metabolic signatures between euploid and aneuploid human blastocysts.

Human Reproduction, 2022
Jaimin Shah, Tim Sanchez, Alan S Penzias
exaly  

Special issue on fluorescence lifetime imaging (FLIM): from fundamentals to applications

Methods and Applications in Fluorescence, 2020
Simon Ameer-Beg, Klaus Suhling, Marina Kuimova   +2 more
openaire   +3 more sources

Phasor S-FLIM: a new paradigm for fast and robust spectral fluorescence lifetime imaging

Nature Methods, 2021
Enrico Gratton, Lorenzo Scipioni, Giulia Tedeschi   +2 more
exaly  

Fluorescence Lifetime-Resolved Imaging Microscopy (FLIM)

1996
R. M. Clegg, P. C. Schneider, T. M. Jovin   +2 more
openaire   +1 more source