Results 1 to 10 of about 307,855 (199)

Engineering the fragment crystallizable (Fc) region of human IgG1 multimers and monomers to fine-tune interactions with sialic acid-dependent receptors [PDF]

Journal of Biological Chemistry, 2017
Multimeric fragment crystallizable (Fc) regions and Fc-fusion proteins are actively being explored as biomimetic replacements for IVIG therapy, which is deployed to manage many diseases and conditions but is expensive and not always efficient. The Fc region of human IgG1 (IgG1-Fc) can be engineered into multimeric structures (hexa-Fcs) that bind their ...
Patricia A. Blundell, Ngoc Phuong Lan Le, Joel Allen, Yasunori Watanabe, Richard J. Pleass   +4 more
semanticscholar   +6 more sources

Choice of host cell line is essential for the functional glycosylation of the fragment crystallizable (Fc) region of human IgG1 inhibitors of influenza B viruses [PDF]

bioRxiv, 2019
AbstractAntibodies are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc, whose presence and fine structure profoundly impacts on theirin vivoimmunogenicity, pharmacokinetics and functional attributes. The host cell line used to produce IgG has a major impact on this glycosylation, as different systems express different ...
Blundell, Patricia A., Lu, Dongli, Dell, Anne, Haslam, Stuart M., Pleass, Richard J.   +4 more
semanticscholar   +5 more sources

A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors [PDF]

Methods in molecular biology, 2018
Engineering the fragment crystallizable (Fc) of human IgG can bring improved effector functions to monoclonal antibodies and Fc-fusion-based medicines and vaccines. Such Fc-effector functions are largely controlled by posttranslational modifications (PTMs) within the Fc, including the addition of glycans that introduce structural and functional ...
Richard J. Pleass, Patricia A. Blundell
semanticscholar   +5 more sources

Identification of conformational epitopes on fragment crystallizable region of human Immunoglobulin G by immunoinformatic

Tehran University Medical Journal, 2018
Background: Immunoglobulins are a group of proteins have important role in defense against microorganisms. Human immunoglobulins are divided into five classes: IgA, IgM, IgD, IgE and IgG.
Soheyla Rohani, Fatemeh Hajighasemi, Fatemeh Sefid   +2 more
doaj   +2 more sources

Impact of LS Mutation on Pharmacokinetics of Preventive HIV Broadly Neutralizing Monoclonal Antibodies: A Cross-Protocol Analysis of 16 Clinical Trials in People without HIV [PDF]

Pharmaceutics
Monoclonal antibodies are commonly engineered with an introduction of Met428Leu and Asn434Ser, known as the LS mutation, in the fragment crystallizable region to improve pharmacokinetic profiles.
Bryan T. Mayer, Lily Zhang, Allan C. deCamp, Chenchen Yu, Alicia Sato, Heather Angier, Kelly E. Seaton, Nicole Yates, Julie E. Ledgerwood, Kenneth Mayer, Marina Caskey, Michel Nussenzweig, Kathryn Stephenson, Boris Julg, Dan H. Barouch, Magdalena E. Sobieszczyk, Srilatha Edupuganti, Colleen F. Kelley, M. Juliana McElrath, Huub C. Gelderblom, Michael Pensiero, Adrian McDermott, Lucio Gama, Richard A. Koup, Peter B. Gilbert, Myron S. Cohen, Lawrence Corey, Ollivier Hyrien, Georgia D. Tomaras, Yunda Huang   +29 more
doaj   +3 more sources

Pre-Clinical Studies of a Novel Bispecific Fusion Protein Targeting C3b and VEGF in Neovascular and Nonexudative AMD Models [PDF]

Ophthalmology and Therapy
Introduction KNP-301 is a bi-specific fragment crystallizable region (Fc) fusion protein, which inhibits both C3b and vascular endothelial growth factor (VEGF) simultaneously for patients with late-stage age-related macular degeneration (AMD).
Yeri Lee, Donggeon Kim, Philip E. D. Chung, Minkyeong Lee, Nahmju Kim, Jihoon Chang, Byoung Chul Lee   +6 more
doaj   +3 more sources

Local Immune Activation and Age Impact on Humoral Immunity in Mice, with a Focus on IgG Sialylation [PDF]

Vaccines
Age alters the host’s susceptibility to immune induction. Humoral immunity with circulating antibodies, particularly immunoglobulin G (IgG), plays an essential role in immune response.
Priti Gupta, Tibor Sághy, Miriam Bollmann, Tao Jin, Claes Ohlsson, Hans Carlsten, Carmen Corciulo, Cecilia Engdahl   +7 more
doaj   +3 more sources

Identification of mpox M1R and B6R monoclonal and bispecific antibodies that efficiently neutralize authentic mpox virus [PDF]

Emerging Microbes and Infections
In 2022, the monkeypox virus (mpox virus, MPXV) exhibited global dissemination across six continents, representing a notable challenge owing to the scarcity of targeted antiviral interventions.
Zuning Ren, Mengjun Li, Jiayin Chen, Xiaohua Gong, Shuo Song, Delin Li, Minghui Yang, Jianhai Yu, Sadia Asghar, Yanxin Cui, Shiyu Niu, Zhonghui Liao, Yushan Jiang, Jiahui Liu, Yuqing Li, Bao Zhang, Wei Zhao, Jie Peng, Yang Yang, Chenguang Shen   +19 more
doaj   +3 more sources

Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions

Nature Biomedical Engineering, 2022
AbstractShort half-lives in circulation and poor transport across the blood–brain barrier limit the utility of cytokines and growth factors acting as receptor agonists. Here we show that surrogate receptor agonists with longer half-lives in circulation and enhanced transport rates across the blood–brain barrier can be generated by genetically inserting
Katsuya Sakai, Nozomi Sugano-Nakamura, Emiko Mihara, Nichole Marcela Rojas-Chaverra, Sayako Watanabe, Hiroki Sato, Ryu Imamura, Dominic Chih-Cheng Voon, Itsuki Sakai, Chihiro Yamasaki, Chise Tateno, Mikihiro Shibata, Hiroaki Suga, Junichi Takagi, Kunio Matsumoto   +14 more
openaire   +3 more sources

Determination of variable region sequences from hybridoma immunoglobulins that target Mycobacterium tuberculosis virulence factors.

PLoS ONE, 2021
Mycobacterium tuberculosis (Mtb) infects one-quarter of the world's population. Mtb and HIV coinfections enhance the comorbidity of tuberculosis (TB) and AIDS, accounting for one-third of all AIDS-associated mortalities.
Hui-Chen Chang Foreman, Andrew Frank, Timothy T Stedman   +2 more
doaj   +2 more sources