Results 1 to 10 of about 7,556 (242)

Engineering the fragment crystallizable (Fc) region of human IgG1 multimers and monomers to fine-tune interactions with sialic acid-dependent receptors [PDF]

Journal of Biological Chemistry, 2017
Multimeric fragment crystallizable (Fc) regions and Fc-fusion proteins are actively being explored as biomimetic replacements for IVIG therapy, which is deployed to manage many diseases and conditions but is expensive and not always efficient. The Fc region of human IgG1 (IgG1-Fc) can be engineered into multimeric structures (hexa-Fcs) that bind their ...
Patricia A. Blundell, Ngoc Phuong Lan Le, Joel Allen, Yasunori Watanabe, Richard J. Pleass   +4 more
openaire   +5 more sources

Choice of host cell line is essential for the functional glycosylation of the fragment crystallizable (Fc) region of human IgG1 inhibitors of influenza B viruses [PDF]

, 2019
AbstractAntibodies are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc, whose presence and fine structure profoundly impacts on theirin vivoimmunogenicity, pharmacokinetics and functional attributes. The host cell line used to produce IgG has a major impact on this glycosylation, as different systems express different ...
Blundell, Patricia A., Lu, Dongli, Dell, Anne, Haslam, Stuart M., Pleass, Richard J.   +4 more
openaire   +4 more sources

A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors [PDF]

, 2018
Engineering the fragment crystallizable (Fc) of human IgG can bring improved effector functions to monoclonal antibodies and Fc-fusion-based medicines and vaccines. Such Fc-effector functions are largely controlled by posttranslational modifications (PTMs) within the Fc, including the addition of glycans that introduce structural and functional ...
Richard J. Pleass, Patricia A. Blundell
openaire   +4 more sources

Altered IgG N-Glycosylation at Onset of Type 1 Diabetes in Children Is Predominantly Driven by Changes in the Fab N-Glycans [PDF]

Biomedicines
Background: N-glycosylation is a post-translational modification involving the attachment of oligosaccharides to proteins and is known to influence immunoglobulin G (IgG) effector functions and even antigen binding.
Branimir Plavša, Najda Rudman, Flemming Pociot, Olga Gornik   +3 more
doaj   +2 more sources

Certolizumab pegol in severe hidradenitis suppurativa in pregnancy: A case report [PDF]

SAGE Open Medical Case Reports
Hidradenitis suppurativa is a chronic inflammatory disease of the skin with a suppurative-cicatricial outcome affecting the infundibular component of the pilo-sebaceous unit.
Paolo Gisondi, Elena Montalto, Tea Curic, Elisabetta Danese, Francesco Bellinato, Giampiero Girolomoni   +5 more
doaj   +2 more sources

Humanized VHH-hFc Fusion Proteins Targeting the L-HN Fragment of Tetanus Toxin Provided Protection In Vivo [PDF]

Antibodies
Background: Tetanus toxin, produced by Clostridium tetani, is the second deadliest known toxin. Antibodies capable of neutralizing tetanus toxin (TeNT) are vital for preventing and treating tetanus disease.
Yating Li, Kexuan Cheng, Jiazheng Guo, Yujia Jiang, Qinglin Kang, Rong Wang, Peng Du, Chen Gao, Yunzhou Yu, Zhixin Yang, Wei Wang, Jiansheng Lu   +11 more
doaj   +2 more sources

Rapid In Vivo Screening of Monoclonal Antibody Cocktails Using Hydrodynamic Delivery of DNA-Encoded Modified Antibodies [PDF]

Biomedicines
Background: Monoclonal antibodies (mAbs) are potent treatment options for infectious diseases. The rapid isolation and in vivo validation of therapeutic mAb candidates, including mAb cocktails, are essential to combat novel or rapidly mutating pathogens.
Hugues Fausther-Bovendo, George (Giorgi) Babuadze, Teodora Ivanciuc, Birte Kalveram, Yue Qu, Jihae Choi, Allison McGeer, Mario Ostrowski, Samira Mubareka, Ami Patel, Roberto P. Garofalo, Robert Kozak, Gary P. Kobinger   +12 more
doaj   +2 more sources

Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions

Nature Biomedical Engineering, 2022
AbstractShort half-lives in circulation and poor transport across the blood–brain barrier limit the utility of cytokines and growth factors acting as receptor agonists. Here we show that surrogate receptor agonists with longer half-lives in circulation and enhanced transport rates across the blood–brain barrier can be generated by genetically inserting
Katsuya Sakai, Nozomi Sugano-Nakamura, Emiko Mihara, Nichole Marcela Rojas-Chaverra, Sayako Watanabe, Hiroki Sato, Ryu Imamura, Dominic Chih-Cheng Voon, Itsuki Sakai, Chihiro Yamasaki, Chise Tateno, Mikihiro Shibata, Hiroaki Suga, Junichi Takagi, Kunio Matsumoto   +14 more
openaire   +2 more sources

Enhancement of SARS-CoV-2 Infection via Crosslinking of Adjacent Spike Proteins by N-Terminal Domain-Targeting Antibodies

Viruses, 2023
The entry of SARS-CoV-2 into host cells is mediated by the interaction between the spike receptor-binding domain (RBD) and host angiotensin-converting enzyme 2 (ACE2).
Tina Lusiany, Tohru Terada, Jun-ichi Kishikawa, Mika Hirose, David Virya Chen, Fuminori Sugihara, Hendra Saputra Ismanto, Floris J. van Eerden, Songling Li, Takayuki Kato, Hisashi Arase, Matsuura Yoshiharu, Masato Okada, Daron M. Standley   +13 more
doaj   +1 more source

In vivo pharmacokinetic enhancement of monomeric Fc and monovalent bispecific designs through structural guidance

Communications Biology, 2021
Lu Shan et al. present a structure-guided approach to engineer a monovalent form of the fragment crystallizable (Fc) region of an IgG4 antibody to adapt multiple versions of half-life extension modifications and bispecific targeting.
Lu Shan, Nydia Van Dyk, Nantaporn Haskins, Kimberly M. Cook, Kim L. Rosenthal, Ronit Mazor, Sonia Dragulin-Otto, Yu Jiang, Herren Wu, William F. Dall’Acqua, Martin J. Borrok, Melissa M. Damschroder, Vaheh Oganesyan   +12 more
doaj   +1 more source