Results 11 to 20 of about 881 (146)

Circulating DNA fragmentomics and cancer screening

Cell Genomics, 2023
Summary: The high fragmentation of nuclear circulating DNA (cirDNA) relies on chromatin organization and protection or packaging within mononucleosomes, the smallest and the most stabilized structure in the bloodstream.
A.R. Thierry
exaly   +6 more sources

Cell-Free DNA Fragmentomics in Liquid Biopsy

Diagnostics, 2022
Cell-free DNA (cfDNA) in bodily fluids has rapidly transformed the development of noninvasive prenatal testing, cancer liquid biopsy, and transplantation monitoring. Plasma cfDNA consists of a mixture of molecules originating from various bodily tissues.
Spencer C Ding, Y M Dennis Lo, Y M Dennis Lo   +2 more
exaly   +5 more sources

Analysis of cfDNA fragmentomics metrics and commercial targeted sequencing panels [PDF]

Nature Communications
Fragmentomics based analysis of cell-free DNA (cfDNA) has recently emerged as a method to infer epigenetic and transcriptional data. Many of these reports analyze whole genome sequencing (WGS) which is not readily available clinically.
Kyle T. Helzer, Marina N. Sharifi, Jamie M. Sperger, Matthew R. Chrostek, Matthew L. Bootsma, Shannon R. Reese, Amy Taylor, Katie R. Kaufmann, Hannah Krause, Jennifer Schehr, Nan Sethakorn, David Kosoff, Christos E. Kyriakopoulos, Michael Bassetti, Grace Blitzer, John Floberg, Martin Sjöström, Andrew J. Armstrong, Himisha Beltran, Rana R. McKay, Felix Y. Feng, Ruth O’Regan, Kari B. Wisinski, Hamid Emamekhoo, Alex W. Wyatt, Joshua M. Lang, Shuang G. Zhao   +26 more
doaj   +5 more sources

Liquid biopsy epigenetics: establishing a molecular profile based on cell‐free DNA [PDF]

Molecular Oncology
Liquid biopsies containing circulating tumor DNA (ctDNA) are important biomarkers across several forms of cancer. The detection of mutations in cell‐free DNA (cfDNA) indicates the presence of ctDNA.
Christoffer Trier Maansson, Anders Lade Nielsen, Boe Sandahl Sorensen   +2 more
doaj   +3 more sources

A multi-center study for colorectal cancer early detection in high-risk disease patients using cell-free fragmentomics assay [PDF]

Cancer Cell International
Background Early detection of colorectal cancer (CRC) is crucial for improving patient survival. This innovative multi-center study aims to develop a non-invasive blood-based assay using cell-free DNA (cfDNA) fragmentomics to differentiate CRC from ...
Bing Gu, Dongqin Zhu, Xiuxiu Xu
exaly   +3 more sources

Cancer-Like Fragmentomic Characteristics of Somatic Variants in Cell-Free DNA. [PDF]

Adv Sci (Weinh)
We report that in non‐cancerous subjects, cell‐free (cfDNA) molecules harboring somatic variants exhibit cancer‐like fragmentomic characteristics associated with clonal hematopoiesis. Importantly, these somatic variant‐associated fragmentomic aberrations are more pronounced in cancer patients. Leveraging such somatic variant‐associated signals in cfDNA,
Zhang Z, An Y, Yang M, Pan Y, Liu X, Gong F, Lin H, Tang B, Bai Y, Zhao X, Zhao Y, Du C, Sun K.   +12 more
europepmc   +4 more sources

Seminal plasma cfDNA fragmentomics landscape delineates male infertility subtypes [PDF]

Clinical Epigenetics
Background While cell-free DNA (cfDNA) fragmentomics has transformed liquid biopsy applications in prenatal screening and oncology, its potential in male reproductive health remains unexplored.
Xiaoyu Wu, Fan Meng, Huiping Zhang, Zeqing Li, Kai Zhao   +4 more
doaj   +4 more sources

Longitudinal profiling of hormone receptor positive, HER2 negative metastatic breast cancer through droplet digital PCR-based circulating tumor DNA fragmentomics [PDF]

Translational Oncology
Background: In the context of hormone receptor positive, HER2 negative Metastatic breast cancer (MBC), CDK 4/6 inhibitors (CDK4/6i) combined with endocrine therapy represent the standard first-line treatment, improving Progression-Free Survival (PFS) and
Alessandro Minisini, Fabiola Giudici, Arianna Dri   +2 more
exaly   +4 more sources

Quantitative fragmentomics allow affinity mapping of interactomes [PDF]

Nature Communications, 2022
Protein networks have been widely explored but most binding affinities remain unknown, limiting the quantitative interpretation of interactomes. Here the authors measure affinities of 65,000 interactions involving human PDZ domains and target sequence ...
Gergo Gogl, Boglarka Zambo, Camille Kostmann, Alexandra Cousido-Siah, Bastien Morlet, Fabien Durbesson, Luc Negroni, Pascal Eberling, Pau Jané, Yves Nominé, Andras Zeke, Søren Østergaard, Élodie Monsellier, Renaud Vincentelli, Gilles Travé   +14 more
doaj   +4 more sources

Cell-Free DNA Fragmentomics: The Novel Promising Biomarker

International Journal of Molecular Sciences, 2023
Cell-free DNA molecules are released into the plasma via apoptotic or necrotic events and active release mechanisms, which carry the genetic and epigenetic information of its origin tissues. However, cfDNA is the mixture of various cell fragments, and the efficient enrichment of cfDNA fragments with diagnostic value remains a great challenge for ...
Qinyu Ge, Min Pan, Yunfei Bai
exaly   +3 more sources