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Nucleocytoplasmic shuttling revealed by FRAP and FLIP technologies

Current Opinion in Biotechnology, 2005
Protein mobility within cells is of key importance for many cellular functions. Although immunostaining can reveal protein locations in the steady-state, this might not represent the full picture and provides no information about protein movements. Fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) are two ...
Mario, Köster   +2 more
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Auswertung von FRAP-Experimenten

2015
Die Auswertung von FRAP-Experimenten nach AXELROD et al. [3] wird noch heute haufig verwendet, da sie relativ leicht durchzufuhren ist. Im Folgenden werden die Grundzuge der theoretischen Beschreibung eines FRAP-Experiments nach AXELROD et al. dargestellt, dabei wird nur auf seine Uberlegungen zur lateralen Diffusion in planaren Lipidmembranen ...
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Simulation von FRAP-Experimenten

2015
Um die zuvor theoretisch beschriebenen Methoden einem Test zu unterziehen, sollen in diesem Kapitel Simulationen von FRAP-Experimenten durchgefuhrt werden. Die Simulation der Experimente wird mit der Programmiersprache PYTHON durchgefuhrt, speziell werden die PYTHON-Bibliotheken SCIPY[20] und NUMPY[20] fur numerische Berechnungen, Integrationen ...
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Molecular diffusion and binding analyzed with FRAP

Protoplasma, 2014
Intracellular molecular transport and localization are crucial for cells (plant cells as much as mammalian cells) to proliferate and to adapt to diverse environmental conditions. Here, some aspects of the microscopy-based method of fluorescence recovery after photobleaching (FRAP) are introduced.
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FRAP

Imaging & Microscopy, 2008
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"Frap"

Notes and Queries, 1914
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Systematic Analysis of FRAP Experiments

Imaging & Microscopy, 2007
Sebastian Seiffert   +2 more
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