Results 201 to 210 of about 66,491 (250)

Expanding the scope of quantitative FRAP analysis

Journal of Theoretical Biology, 2010
In this study, new mathematical models were developed for analysis of fluorescence recovery after photobleaching (FRAP) data to account for features not represented in previous analysis: conical photobleaching geometry, spatial variations in binding of fluorescent molecules, and directed transport of fluorescent molecules. To facilitate computations in
Mark A, Hallen, Anita T, Layton
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Analysis of receptor oligomerization by FRAP microscopy

Nature Methods, 2009
Here we describe an approach to investigate di- or oligomerization of transmembrane receptors in living cells with fluorescence recovery after photobleaching (FRAP). We immobilized a defined fraction of receptors with antibodies and then measured lateral mobility of the nonimmobilized fraction by FRAP.
Dorsch, Sandra, Klotz, Karl-Norbert, Engelhardt, Stefan, Lohse, Martin J, Bünemann, Moritz   +4 more
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Molecular diffusion and binding analyzed with FRAP

Protoplasma, 2014
Intracellular molecular transport and localization are crucial for cells (plant cells as much as mammalian cells) to proliferate and to adapt to diverse environmental conditions. Here, some aspects of the microscopy-based method of fluorescence recovery after photobleaching (FRAP) are introduced.
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Nucleocytoplasmic shuttling revealed by FRAP and FLIP technologies

Current Opinion in Biotechnology, 2005
Protein mobility within cells is of key importance for many cellular functions. Although immunostaining can reveal protein locations in the steady-state, this might not represent the full picture and provides no information about protein movements. Fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) are two ...
Mario, Köster, Thomas, Frahm, Hansjörg, Hauser   +2 more
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Analysis of Biomolecular Dynamics by FRAP and Computer Simulation

2014
We present a Monte Carlo simulation environment for modelling complex biological molecular interaction networks and for the design, validation, and quantitative analysis of FRAP assays to study these. The program is straightforward in its implementation and can be instructed through an intuitive script language.
Geverts, Bart, Van Royen, Martin E., Houtsmuller, Adriaan B.   +2 more
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FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos

2018
Morphogens are signaling molecules that provide positional information to cells during development. They must move through embryonic tissues in order to coordinate patterning. The rate of a morphogen's movement through a tissue-its effective diffusivity-affects the morphogen's distribution and therefore influences patterning.
Soh, Gary H., Müller, Patrick
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Once a physicist: Thomas Fraps

Physics World, 2006
My interest in science was first sparked by my grandfather, who was an engineer and mathematician. Later I started reading science-fiction and popular-science books by the likes of Carl Sagan and Douglas Hofstadter. I finally decided to study physics when I was doing my military service, which would have been an intellectual vacuum for me had it not ...
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Auswertung von FRAP-Experimenten

2015
Die Auswertung von FRAP-Experimenten nach AXELROD et al. [3] wird noch heute haufig verwendet, da sie relativ leicht durchzufuhren ist. Im Folgenden werden die Grundzuge der theoretischen Beschreibung eines FRAP-Experiments nach AXELROD et al. dargestellt, dabei wird nur auf seine Uberlegungen zur lateralen Diffusion in planaren Lipidmembranen ...
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Complex Applications of Simple FRAP on Membranes

2009
There is an old adage that says “To see is to believe,” and it still seems to be true in many fields of biology. For an experimental validation of hypotheses, modern biology takes advantage of various fluorescence-based techniques (fluorescence microscopy, digital image analysis) for visualization and quantification.
Minchul Kang, Anne K. Kenworthy
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Simulation von FRAP-Experimenten

2015
Um die zuvor theoretisch beschriebenen Methoden einem Test zu unterziehen, sollen in diesem Kapitel Simulationen von FRAP-Experimenten durchgefuhrt werden. Die Simulation der Experimente wird mit der Programmiersprache PYTHON durchgefuhrt, speziell werden die PYTHON-Bibliotheken SCIPY[20] und NUMPY[20] fur numerische Berechnungen, Integrationen ...
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