Results 71 to 80 of about 3,115 (208)
Efficient Biosynthesis of 2’-Fucosyllactose Using Glycerol and Lactose in Engineered Escherichia coli [PDF]
Shipin KexueIn this study, the de novo synthesis pathway of 2’-FL was established in Escherichia coli BL21star(DE3). The β-galactosidase gene (lacZ) and the UDP-glucose lipid carrier transferase gene (wcaJ) were knocked out by the CRISPR/Cas9 system.
HE Zi, LIANG Shanquan, LIU Dan, JIANG Yawen, JIANG Zhengqiang, YANG Shaoqing
doaj +1 more source
Fucosyltransferase 4 shapes oncogenic glycoproteome to drive metastasis of lung adenocarcinoma
EBioMedicine, 2020 Background: Aberrant fucosylation plays a critical role in lung cancer progression. Nevertheless, the key fucosyltransferase with prognostic significance in lung cancer patients, the enzyme's intracellular targets, and complex molecular mechanisms ...
Hsuan-Hsuan Lu +13 more
doaj +1 more source
Naïve T cells re-distribute to the lungs of selectin ligand deficient mice. [PDF]
PLoS ONE, 2010 Selectin mediated tethering represents one of the earliest steps in T cell extravasation into lymph nodes via high endothelial venules and is dependent on the biosynthesis of sialyl Lewis X (sLe(x)) ligands by several glycosyltransferases, including two ...
John R Harp, Thandi M Onami
doaj +1 more source
Protein Posttranslational Modifications in Immunity: Molecular Mechanisms and Therapeutic Targets
MedComm, Volume 7, Issue 9, September 2026.Protein posttranslational modifications, including phosphorylation, ubiquitination, SUMOylation, acetylation, methylation, glycosylation, and other new types, dynamically regulate antigen presentation and recognition, immune signal transduction, antibody production and class switching, immune contraction, immune memory establishment, as well as immune ...
Yuxing Yao +10 more
wiley +1 more source
, 2013 The α-(1,2) fucosyltransferases (Fut1 and Fut2) and α-(1,3) fucosyltransferases (Fut4, Fut9) are responsible for the synthesis of Lewis X (LeX) and Lewis Y (LeY) conjugated to glycoproteins.
Wu-Bin Ma +11 more
core +1 more source
Protein O-Fucosyltransferases: Biological Functions and Molecular Mechanisms in Mammals
MoleculesDomain-specific O-fucosylation is an unusual type of glycosylation, where the fucose is directly attached to the serine or threonine residues in specific protein domains via an O-linkage.
Huilin Hao +3 more
doaj +1 more source
QTL study reveals candidate genes underlying host resistance in a Red Queen model system.
PLoS Genetics, 2023 Specific interactions of host and parasite genotypes can lead to balancing selection, maintaining genetic diversity within populations. In order to understand the drivers of such specific coevolution, it is necessary to identify the molecular ...
Maridel Fredericksen +4 more
doaj +1 more source
High‐Content CRISPR Screening: Methods and Applications
MedComm, Volume 7, Issue 9, September 2026.High‐content CRISPR screening represents a paradigm shift in functional genomics, moving beyond traditional survival‐based readouts to enable multidimensional mapping of genotype–phenotype relationships. This review systematically outlines the methodological evolution of this approach, detailing advances in perturbation modalities, delivery systems ...
Yike Zhang +6 more
wiley +1 more source
The Plant Genome, Volume 19, Issue 3, September 2026.Abstract Red clover (Trifolium pratense L.) is a globally important temperate forage legume. Its symbiosis with soil‐borne rhizobia enables nitrogen fixation, and its ability to produce quality forage under diverse soil conditions enhances pasture productivity, particularly during water deficits.
A. D. Heslop +11 more
wiley +1 more source
, 2016 Two recombinant fucosyltransferases were employed as synthetic tools in the chemoenzymatic synthesis of core fucosylated N-glycan structures. Enzyme substrates were rapidly identified by incubating a microarray of synthetic N-glycans with the ...
Shi Yan (164233) +4 more
core +1 more source