Results 81 to 90 of about 5,422 (250)

Human α1,3/4-Fucosyltransferases [PDF]

Journal of Biological Chemistry, 1998
An analysis of the acceptor substrate specificity of domain swap mutants of human alpha1,3/4-fucosyltransferases (FucTs) III and V has been carried out. The results demonstrate that changing Asp336 of FucT III to Ala (as in FucT V) produced a protein (III/V1) with a reduced activity with a variety of acceptors.
Loc Vo, Stephanie Lee, Eric H. Holmes, Marie C. Marcinko, Bruce A. Macher   +4 more
openaire   +2 more sources

Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Cancer [PDF]

, 2023
project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. Publisher Copyright: © 2023 by the authors.Triple-negative breast cancer (TNBC) encompasses multiple entities and is generally highly aggressive and metastatic.
Barreira, Daniela F., Borralho, Paula, Braga, Sofia, Carrascal, Mylène A., Granjo, Pedro, Grosso, Ana R., Lourenço, Rita A., Pascoal, Carlota, Videira, Paula A.   +8 more
core   +1 more source

Red Alga Porphyridium Supports High‐Yield Production of a Functional Chimeric Hepatitis B Surface Antigen With Strong Cellular and Humoral Immunogenicity

Plant Biotechnology Journal, EarlyView.
ABSTRACT Microalgae represent promising production factories for the light‐driven, cost‐effective production of recombinant proteins. The red microalga Porphyridium purpureum displays particularly favourable transgene expression properties due to the episomal maintenance of transformation vectors at high copy numbers in the nucleus.
Ana‐Maria Pantazica, Alexander Hammel, Iuliana Caras, Irina Ionescu, Catalin Tucureanu, Adrian Onu, Maria Murace, Jihong Liu Clarke, Crina Stavaru, Norica Branza‐Nichita, Ralph Bock   +10 more
wiley   +1 more source

Elevation of α-1,3 fucosylation promotes the binding ability of TNFR1 to TNF-α and contributes to osteoarthritic cartilage destruction and apoptosis

Arthritis Research & Therapy, 2022
Background Osteoarthritis (OA) is the most common form of arthritis and is characterized by the degradation of articular cartilage and inflammation of the synovial membrane.
Hanjie Yu, Mingxiu Li, Xiaodong Wen, Jie Yang, Xiaojun Liang, Xia Li, Xiaojuan Bao, Jian Shu, Xiameng Ren, Wentian Chen, Zheng Li, Yi Li   +11 more
doaj   +1 more source

Trafficking and localization studies of recombinant α1,3-fucosyltransferase VI stably expressed in CHO cells [PDF]

, 2017
Peripheral α1,3-fucosylation of glycans occurs by the action of either one of five different α1,3-fucosyltransferases (Fuc-Ts) cloned to date. Fuc-TVI is one of the α1,3-fucosyltransferases which is capable to synthesize selectin ligands.
Berger, Eric G., Borsig, Lubor, Bowen, Benjamin R., Katopodis, Andreas G.   +3 more
core  

CRISPR/Cas9‐Mediated Knockouts of the ALG3 and GNTI in N. benthamiana and Their Application to Pharmaceutical Production

Plant Biotechnology Journal, EarlyView.
ABSTRACT N‐Glycosylation critically influences the efficacy, safety and pharmacokinetic properties of biopharmaceuticals. Plant expression platforms offer multiple advantages for the production of N‐glycosylated proteins, but their use is impeded by the presence of plant‐specific N‐glycan epitopes, which raise concerns of possible immunogenicity to ...
Dolgormaa Bataa, Hiroyuki Kajiura, Reimi Lai Sang Sawada‐Choi, Yukino Yamashita, Takeshi Ishimizu, Ryo Misaki, Atsushi Takeda, Kazuhito Fujiyama   +7 more
wiley   +1 more source

High‐Throughput Screening for Inhibitors of Sialyl‐ and Fucosyltransferases [PDF]

Angewandte Chemie, 2011
Sweet screens: A high-throughput screening platform for identification of inhibitors of sialyl- and fucosyltransferases based on fluorescence polarization (FP) has been developed. An analogue of the natural donor substrate carrying a fluorescent label (green star) is transferred to a glycoprotein acceptor, which results in robust FP.
James C. Paulson, Cory D. Rillahan, Steven J. Brown, Ames C. Register, Hugh Rosen   +4 more
openaire   +3 more sources

Identification of the Plasticity-Relevant Fucose-α(1−2)-Galactose Proteome from the Mouse Olfactory Bulb [PDF]

, 2009
Fucose-α(1−2)-galactose [Fucα(1−2)Gal] sugars have been implicated in the molecular mechanisms that underlie neuronal development, learning, and memory. However, an understanding of their precise roles has been hampered by a lack of information regarding
Domino, Steven E., Ficarro, Scott B., Hsieh-Wilson, Linda C., Krishnamurthy, Chithra, Murrey, Heather E., Peters, Eric C.   +5 more
core   +4 more sources

Heterologous Production of Cyprosin B in Nicotiana benthamiana: Unveiling the Role of the Plant‐Specific Insert Domain in Protein Function and Subcellular Localisation

Plant Biotechnology Journal, EarlyView.
ABSTRACT Plant systems have gained increased attention as an alternative platform for producing heterologous proteins, particularly for industrially relevant proteins. The Cynara cardunculus L. flower extract is traditionally used in cheese production across Mediterranean countries due to its milk‐clotting properties.
Saraladevi Muthusamy, Ramesh Raju Vetukuri, Anneli Lundgren, Sungyong Kim, Pruthvi B. Kalyandurg, Åke Strid, Li‐Hua Zhu, Selvaraju Kanagarajan, Peter E. Brodelius   +8 more
wiley   +1 more source

The impact of elevation of total bilirubin level and etiology of the liver disease on serum N-glycosylation patterns in mice and men [PDF]

, 2010
The GlycoFibroTest and GlycoCirrhoTest are noninvasive alternatives for liver biopsy that can be used as a follow-up tool for fibrosis patients and to diagnose cirrhotic patients, respectively.
BLOMME, BRAM, Callewaert, Nico, Colle, Isabelle, Van Steenkiste, Christophe, Van Vlierberghe, Hans, Vanhuysse, Jacques   +5 more
core   +1 more source