Biochemical characterization of three Aspergillus niger β-galactosidases
Background: β-Galactosidases catalyze both hydrolytic and transgalactosylation reactions and therefore have many applications in food, medical, and biotechnological fields.
Dandan Niu+7 more
doaj +1 more source
Understanding the Effects of Lactose Hydrolysis Modeling on the Main Oligosaccharides in Goat Milk Whey Permeate. [PDF]
Enzymatic hydrolysis of lactose is a crucial step to improve the efficiency and selectivity of membrane-based separations toward the recovery of milk oligosaccharides free from simple sugars.
Barile, Daniela+5 more
core +2 more sources
Improving the Secretory Expression of an -Galactosidase from Aspergillus niger in Pichia pastoris. [PDF]
α-Galactosidases are broadly used in feed, food, chemical, pulp, and pharmaceutical industries. However, there lacks a satisfactory microbial cell factory that is able to produce α-galactosidases efficiently and cost-effectively to date, which prevents ...
Xianliang Zheng+6 more
doaj +1 more source
Recombinant microbial systems for improved β-galactosidase production and biotechnological applications [PDF]
β-Galactosidases (EC 3.2.1.23) constitute a large family of proteins that are known to catalyze both hydrolytic and transgalactosylation reactions. The hydrolytic activity has been applied in the food industry for decades for reducing the lactose content
Domingues, Lucília+2 more
core +1 more source
The red palm weevil, Rhynchophorus ferrugineus (Olivier), is one of the most destructive pests of palm trees in the southeast of Iran. Digestion in the alimentary canal of the red palm weevil is facilitated by some carbohydrases.
Nasir Saberi RISEH+2 more
doaj +1 more source
DENATURATION OF NATIVE AND DEGLYCOSYLATED α-GALACTOSIDASES FROM Penicillium canescens BY GUANIDINE HYDROCHLORIDE [PDF]
The aim of this work was the study of native and galactosidases from Penicillium canescens under denaturing conditions caused by guanidine hydrochloride.
Borzova N. V.
doaj +1 more source
Cloning and expression of a thermostable α-galactosidase from the thermophilic fungus Talaromyces emersonii in the methylotrophic yeast Pichia pastoris [PDF]
The first gene (alpha-gal1) encoding an extracellular alpha-Dgalactosidase from the thermophilic fungus Talaromyces emersonii was cloned and characterized.
Fernandes, Sara+4 more
core +1 more source
Cerebroside galactosidase of brain
The galactoside bond in cerebroside was found to be cleaved by an enzyme in rat and pig brain. Emulsified stearoyl-(14)C psychosine was used as the substrate and the extent of cleavage was studied by isolating and counting the stearoyl sphingosine (ceramide) formed.
David M. Bowen+3 more
openaire +4 more sources
Construction, Isolation and Implications of Repressor‐galactosidase ·ß‐galactosidase Hybrid Molecules [PDF]
Escherichia coli heterogenotes, which produce hybrid molecules between the chimaeric protein repressor‐galactosidase and the enzyme β‐galactosidase, were constructed. Repressor‐galactosidase. in which fully active lac repressor is covalently linked to active β‐galactosidase, is an aggregate with a core structure of four β‐galactosidase parts and two ...
Benno Müller-Hill, Jürgen Kania
openaire +3 more sources