Results 21 to 30 of about 1,805 (175)

Combined Transcriptomic and Metabolomic Analysis Reveals an Ethylene-Activated Regulatory Model on Monoterpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus. [PDF]

Plant Biotechnol J
ABSTRACT Catharanthus roseus contains nearly 200 bioactive monoterpenoid indole alkaloids (MIAs) that are effective in treating cancer and other diseases. Ethylene plays a significant role in enhancing MIA biosynthesis, and we have found that it greatly induces the accumulation of anhydrovinblastine.
Deng B, Miao Q, Ou C, Pan Y, Liu H, Fu X, Li L, Wang Y, Tang K, Pan Q.   +9 more
europepmc   +2 more sources

Identification of GBF1 as a cellular factor required for hepatitis E virus RNA replication [PDF]

Cellular Microbiology, 2017
The hepatitis E virus (HEV) genome is a single-stranded, positive-sense RNA that encodes three proteins including the ORF1 replicase. Mechanisms of HEV replication in host cells are unclear, and only a few cellular factors involved in this step have been identified so far.
Rayan Farhat, Maliki Ankavay, Nadjet Lebsir, Jérôme Gouttenoire, Catherine L. Jackson, Czeslaw Wychowski, Darius Moradpour, Jean Dubuisson, Yves Rouillé, Laurence Cocquerel   +9 more
openaire   +4 more sources

GARP dysfunction results in COPI displacement, depletion of Golgi v-SNAREs and calcium homeostasis proteins [PDF]

Frontiers in Cell and Developmental Biology, 2022
Golgi-associated retrograde protein (GARP) is an evolutionary conserved heterotetrameric protein complex that tethers endosome-derived vesicles and is vital for Golgi glycosylation. Microscopy and proteomic approaches were employed to investigate defects
Amrita Khakurel, Tetyana Kudlyk, Irina Pokrovskaya, Zinia D’Souza, Vladimir V. Lupashin   +4 more
doaj   +2 more sources

Acute GARP Depletion Disrupts Vesicle Transport, Leading to Severe Defects in Sorting, Secretion and O-Glycosylation. [PDF]

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Cartoon illustrating defects caused by GARP dysfunction. (Left) Control cells exhibit normal Golgi morphology with a stratified distribution of enzymes and trafficking machinery. (Right) Following acute VPS54 depletion, vesicle numbers increase, the trans‐Golgi enlarges, endosome‐Golgi trafficking proteins mislocalize to vesicles, and Golgi association
Khakurel A, Pokrovskaya I, Aragon-Ramirez WS, Lupashin VV.   +3 more
europepmc   +2 more sources

Disease-Associated Factors at the Endoplasmic Reticulum-Golgi Interface. [PDF]

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The ER–Golgi interface has garnered significant attention due to recent discoveries suggesting potential non‐vesicular transport mechanisms and regulation through liquid‐liquid phase separation. Numerous diseases linked to mutations in proteins located at this interface have been identified, highlighting the importance of comprehensive reviews in this ...
Maeda M, Arakawa M, Saito K.
europepmc   +2 more sources

GBF1 and Arf1 interact with Miro and regulate mitochondrial positioning within cells [PDF]

Scientific Reports, 2018
AbstractThe spatial organization of cells depends on coordination between cytoskeletal systems and intracellular organelles. The Arf1 small G protein and its activator GBF1 are important regulators of Golgi organization, maintaining its morphology and function.
Walch, Laurence, Pellier, Emilie, Leng, Weihua, Lakisic, Goran, Gautreau, Alexis, Contremoulins, Vincent, Verbavatz, Jean-Marc, Jackson, Catherine   +7 more
openaire   +7 more sources

GBF1, a Guanine Nucleotide Exchange Factor for Arf, Is Crucial for Coxsackievirus B3 RNA Replication [PDF]

Journal of Virology, 2009
ABSTRACT The replication of enteroviruses is sensitive to brefeldin A (BFA), an inhibitor of endoplasmic reticulum-to-Golgi network transport that blocks activation of guanine exchange factors (GEFs) of the Arf GTPases. Mammalian cells contain three BFA-sensitive Arf GEFs: GBF1, BIG1, and BIG2.
Lanke, K.H.W., Schaar, H.M. van der, Belov, G.A., Feng, Q., Duijsings, D., Jackson, C.L., Ehrenfeld, E., Kuppeveld, F.J.M. van   +7 more
openaire   +7 more sources

Differential Effects of the Putative GBF1 Inhibitors Golgicide A and AG1478 on Enterovirus Replication [PDF]

Journal of Virology, 2010
ABSTRACT The genus Enterovirus , belonging to the family Picornaviridae , includes well-known pathogens, such as poliovirus, coxsackievirus, and rhinovirus. Brefeldin A (BFA) impedes replication of several enteroviruses through inhibition of Golgi-specific BFA resistance factor 1 ...
Linden, L. van der, Schaar, H.M. van der, Lanke, K.H.W., Neyts, J., Kuppeveld, F.J.M. van   +4 more
openaire   +7 more sources

GBF1- and ACBD3-Independent Recruitment of PI4KIIIβ to Replication Sites by Rhinovirus 3A Proteins [PDF]

Journal of Virology, 2015
ABSTRACT PI4KIIIβ recruitment to Golgi membranes relies on GBF1/Arf and ACBD3. Enteroviruses such as poliovirus and coxsackievirus recruit PI4KIIIβ to their replication sites via their 3A proteins. Here, we show that human rhinovirus (HRV) 3A also recruited PI4KIIIβ to replication sites.
Dorobantu, Cristina M., Ford-Siltz, Lauren A., Sittig, Simone P., Lanke, Kjerstin H. W., Belov, George A., van Kuppeveld, Frank J. M., van der Schaar, Hilde M.   +6 more
openaire   +8 more sources

Kinetics of Arf1 inactivation regulates Golgi organisation and function in non-adherent fibroblasts [PDF]

Biology Open, 2023
Rajeshwari B.R., Nikita Shah, Prachi Joshi, M. S. Madhusudan, Nagaraj Balasubramanian   +4 more
doaj   +2 more sources