Results 151 to 160 of about 954 (183)

Baculovirus-mediated GCRV vp7 and vp6 genes expression in silkworm and grass carp

Molecular Biology Reports, 2016
Grass carp hemorrhagic disease is a common fish disease and often results in significant economic losses in grass carp aquaculture in China. This study was aimed to develop a novel oral vaccine against grass carp reovirus (GCRV). GCRV vp6 and vp7 genes with β-actin promoter of Megalobrama amblycephala and polyhedrin promoter (Ph10) of baculovirus ...
Bo, Liu, Yongchang, Gong, Zhen, Li, Xiaolong, Hu, Guangli, Cao, Renyu, Xue, Chengliang, Gong   +6 more
openaire   +2 more sources

miR-2188-5p promotes GCRV replication by the targeted degradation of klf2a in Ctenopharyngodon idellus

Developmental & Comparative Immunology, 2023
Studies on host immunity evasion by aquatic viruses have largely focused on coding genes. There is accumulating evidence for the important biological functions of non-coding miRNAs in virus-host interactions. The regulatory functions of non-coding miRNAs in fish reovirus-host interactions remain unknown.
Yangyu Li, Liangming Chen, Yangyang Li, Ping Deng, Cheng Yang, Yongming Li, Lanjie Liao, Zuoyan Zhu, Yaping Wang, Rong Huang   +9 more
openaire   +2 more sources

RBX1 and RBX2 promote GCRV replication in grass carp (Ctenopharyngodon idella)

Fish & Shellfish Immunology
Cullin-RING ligases (CRLs) constitute the most diverse E3 ligase family in the ubiquitin-proteasome system, yet their antiviral roles in bony fish remain poorly understood. Here, we identify the catalytic cores of CRLs, the RING-box proteins RBX1 and RBX2, as key modulators of antiviral signaling in cyprinid fish.
Jianhua Feng, Yafang Wang, Wenji Huang, Dawei Xie, Yinghao Huang, Xinran Tao, Tiantian Zhang, Haixia Wu, Jun Zou, Junya Wang   +9 more
openaire   +2 more sources

Irf5 Activates Tbk1 Phosphorylation to Enhance Panoptosis During Gcrv Infectionirf5 Activates Tbk1 Phosphorylation to Enhance Panoptosis During Gcrv Infection

TBK1 is an important IFN antiviral signalling factor, and in previous work black carp TBK1 (bcTBK1) and black carp IRF5 (bcIRF5) together promoted cell death in GCRV-infected cells. In this research, bcTBK1 and bcIRF5 were investigated both in vivo and in vitro to delineate their individual and combined functions.
Can Yang, Jinwei Gao, Hao Wu, Hao Feng, Jun Xiao, Rui Song, Zhonggui Xie, Dongsheng Ou, Yanfang Wu, Qing Yang   +9 more
openaire   +1 more source

The structural characteristics and expression characteristics of C1S in response to GCRV infection in grass carp

Fish & Shellfish Immunology
The complement system, a critical component of innate immunity in fish, plays a pivotal role in the defense against Grass Carp Reovirus (GCRV) infection in grass carp. This study explores the structural characteristics of C1S, a crucial molecule in the classical pathway of the complement system, and its involvement in the response to GCRV infection. We
Yuling Wei, Zhao Lv, Zongjun Du, Tiaoyi Xiao   +3 more
openaire   +2 more sources

Demonstrating the Importance of the Emergence of the S7 Gene in Monitoring GCRV-II Environmental Transmission

Archives of Virology
Grass carp reovirus is a major aquaculture biosecurity threat, causing acute outbreaks and diagnostic delays in hemorrhagic disease. For early warning of GCRV-II in aquaculture water, this study established a method using iron flocculation to concentrate viruses, with qPCR-based tracking and viral load quantification. Results show the S7 gene serves as
Ying Zhan, Jia Yang, Ye Zhang, Liqun Lu, Hao Wang   +4 more
openaire   +2 more sources

IFNa of triploid hybrid of gold fish and allotetraploid is an antiviral cytokine against SVCV and GCRV

Fish & Shellfish Immunology, 2016
Triploid hybrid of gold fish (Carassius auratus red var., ♀, 2n = 100) and allotetroploid (♂, 2n = 100) displays much improved disease resistance than its parents. In this paper, one of the type I IFNs of triploid hybrid (3nIFNa) has been cloned and characterized.
Jun, Yan, Lingzhi, Peng, Yang, Li, Hui, Fan, Yu, Tian, Shaojun, Liu, Hao, Feng   +6 more
openaire   +2 more sources

In Vitro and In Vivo Antiviral Activity of Histone Deacetylase Inhibitors Against GCRV

Journal of Fish Diseases
ABSTRACT Grass carp haemorrhagic disease (GCHD), a severe viral affliction caused by grass carp reovirus (GCRV), is responsible for significant mortality among grass carp. Characterised by rapid transmission and latent infections, GCHD poses a critical challenge in aquaculture.
Guangyao Hu, Feiran Li, Mengsha Zhou, Xiang Tian, Weijun Wu, Hao Wang, Liqun Lu   +6 more
openaire   +2 more sources

Effects of moroxydine hydrochloride and ribavirin on the cellular growth and immune responses by inhibition of GCRV proliferation

Research in Veterinary Science, 2018
Moroxydine hydrochloride (Mor) and ribavirin (Rib) are known for their multi-antiviral activities against DNA and RNA viruses but little information is available about the pharmacological impact in aquaculture. The present study was undertaken to investigate the response of host cells to antiviral compounds during the anti-GCRV treatment.
Xiao-Bo, Yu, Kai, Hao, Jing, Li, Xiao-Hui, Chen, Gao-Xue, Wang, Fei, Ling   +5 more
openaire   +2 more sources

CiLSM14Aa acts as a cytosolic dsRNA sensor to activate antiviral immunity against GCRV-II

Fish & Shellfish Immunology
The innate immune system relies on pattern recognition receptors (PRRs) to detect viral pathogens and initiate antiviral responses. LSM14A, a conserved RNA-binding protein involved in mRNA metabolism, has recently emerged as a cytosolic nucleic acid sensor in vertebrates. However, its role in teleost immunity remains unclear.
Hui Jiang, Chu Zhang, Pengfei Li, Yong-An Zhang, Xiangmou Qin   +4 more
openaire   +2 more sources