Results 151 to 160 of about 1,339 (193)

Autophagy signaling pathway is a therapeutic target to inhibit GCRV replication

Aquaculture, 2022
Abstract Autophagy is an evolutionarily conserved process that is thought to be essential for maintaining physiological homeostasis. However, the role of autophagy in host immune defense against viruses was poorly understood in fish. In this study, we found that although GCRV infection induced autophagy, increasing autophagy level by rapamycin ...
Pengfei Chu, Libo He
exaly   +2 more sources

Nucleophosmin 1a translocated from nucleus to cytoplasm and facilitate GCRV replication

Fish & Shellfish Immunology, 2023
To decipher the functional characterization of Nucleophosmin 1a (NPM1a) from grass carp (Ctenopharyngodon idellus) (CiNPM1a), its cDNA was cloned and bioinformatic analysis were conducted. The full-length cDNA sequence of CiNPM1a is 1732 bp, which encodes 307 amino acids.
Hao, Tang, Mingxue, Sun, Wei, Duan, Yanrong, Ying, Yijing, Liao, Tiaoyi, Xiao, Yaoguo, Li   +6 more
openaire   +2 more sources

Hsp90 Regulates GCRV-II Proliferation by Interacting with VP35 as Its Receptor and Chaperone

Journal of Virology, 2022
Identification of viral receptors has always been the research hot spot in virus research field as receptor functions at the first stage of viral infection, which can be designed as efficient antiviral drug targets. GCRV-II, the causative agent of the grass carp epidemic hemorrhagic disease, has caused tremendous losses in grass carp culture in China.
Lin Jiang, An-qi Liu, Chu Zhang, Yong-An Zhang, Jiagang Tu   +4 more
openaire   +2 more sources

Detection of grass carp reovirus (GCRV) with monoclonal antibodies

Archives of Virology, 2013
Grass carp reovirus (GCRV) is a pathogen that causes hemorrhagic disease of grass carp. It is the most serious infectious disease of carp and causes serious losses of fingerlings of grass carp and black carp. In this study, a recombinant VP4, one of the viral core proteins, was constructed with a histidine tag and expressed at a high level in E.
Jing, Hongli, Zhang, Lifeng, Fang, Zhenzhen, Xu, Lipu, Zhang, Min, Wang, Na, Jiang, Yulin, Lin, Xiangmei   +7 more
openaire   +2 more sources

Functions of MDA5 and its domains in response to GCRV or bacterial PAMPs

Fish & Shellfish Immunology, 2015
Melanoma differentiation-associated gene 5 (MDA5) is a member of retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) family which can initiate type I IFN expression in response to RNA virus infection. In this study, we constructed six mutants of Ctenopharyngodon idella MDA5 (CiMAD5) overexpression plasmids and generated stable transfected C ...
Tianle Gu, Youliang Rao, Jianguo Su, Chunrong Yang, Xiaohui Chen, Lijun Chen, Nana Yan   +6 more
openaire   +2 more sources

bcIRF5 activates bcTBK1 phosphorylation to enhance PANoptosis during GCRV infection

Fish and Shellfish Immunology
TBK1 is an important IFN antiviral signalling factor, and in previous work black carp TBK1 (bcTBK1) and black carp IRF5 (bcIRF5) together promoted cell death in GCRV-infected cells. In this research, bcTBK1 and bcIRF5 were investigated both in vivo and in vitro to delineate their individual and combined functions.
Can Yang
exaly   +3 more sources

ANAX4 is a downstream molecule of LGP2 and promotes GCRV proliferation

Fish & Shellfish Immunology
This study explored the key molecules and signal pathways in the pathogenesis of grass carp reovirus (GCRV). Using immunoprecipitation mass spectrometry and Co-IP validation, the protein CiANXA4 was identified which interacts indirectly with CiLGP2. CiANXA4 encodes 321 amino acids, including 4 ANX domains.
Mingxue Sun, Hao Tang, Tiaoyi Xiao, Yaoguo Li, Yilin Li   +4 more
openaire   +2 more sources

Enhanced Expression of GCRV VP6 in CIK Cells by Relative Sequence Optimization

Applied Biochemistry and Biotechnology, 2014
Efficient expression of target protein is one of strategies for gene therapy or vaccine design. Many studies showed that codon optimization could enhance the expression of target proteins. In this paper, a target sequence of about 1.26 kb encoding the major capsid protein VP6 of grass carp reovirus (GCRV) and an optimized counterpart were synthesized ...
Bo, Xiao, Xiaoyan, Chi, Li, Zhang, Huige, Qu, Yujing, Liu, Xiaojie, Wang, Juhua, Zhou   +6 more
openaire   +2 more sources

Baculovirus-mediated GCRV vp7 and vp6 genes expression in silkworm and grass carp

Molecular Biology Reports, 2016
Grass carp hemorrhagic disease is a common fish disease and often results in significant economic losses in grass carp aquaculture in China. This study was aimed to develop a novel oral vaccine against grass carp reovirus (GCRV). GCRV vp6 and vp7 genes with β-actin promoter of Megalobrama amblycephala and polyhedrin promoter (Ph10) of baculovirus ...
Bo, Liu, Yongchang, Gong, Zhen, Li, Xiaolong, Hu, Guangli, Cao, Renyu, Xue, Chengliang, Gong   +6 more
openaire   +2 more sources