Results 91 to 100 of about 54,378 (282)

Characterization of Novel Integrons, In1085 and In1086, and the Surrounding Genes in Plasmids from Enterobacteriaceae, and the Role for attCaadA16 Structural Features during attI1 × attC Integration

Frontiers in Microbiology, 2017
Novel class 1 integrons In1085 and In1086, containing the class D β-lactamase -encoding gene blaOXA, were identified in clinical enterobacterial strains.
Dongguo Wang, Wei Hou, Jiayu Chen, Linjun Yang, Zhihui Liu, Zhe Yin, Jiao Feng, Dongsheng Zhou   +7 more
doaj   +1 more source

Ultrasound‐Actuated Gene Editing in Human Kidney Organoids

Advanced Science, EarlyView.
Peptide‐stabilized nanoemulsions enable spatially controlled, ultrasound‐triggered delivery of gene‐editing proteins deep within human kidney organoid tissues. Focused acoustic pulses drive droplet vaporization, propelling cargo into target renal cells while preserving native tissue microarchitecture. This non‐viral platform improves both the depth and
Michael A. Miller, Nicole Vo, Utkarsh, Ivan Sokirniy, Justin Pritchard, Benjamin S. Freedman, Scott H. Medina   +6 more
wiley   +1 more source

Characterization of antibiotic resistance genes and mobile genetic elements in Escherichia coli isolated from captive black bears

Scientific Reports
The objective of this study was to analyze the antimicrobial resistance (AMR) characteristics produced by antibiotic resistance genes (ARGs), mobile genetic elements (MGEs) and gene cassettes in Escherichia coli isolated from the feces of captive black ...
Hang Liu, Keyun Shi, Yuhan Wang, Wenhao Zhong, Shulei Pan, Lei Zhou, Yuehong Cheng, Yu Yuan, Ziyao Zhou, Haifeng Liu, Shaqiu Zhang, Guangneng Peng, Qigui Yan, Yan Luo, Xiaoli Zhang, Zhijun Zhong   +15 more
doaj   +1 more source

Integration of Spatiotemporal Multi‐Omics in Peach Fruit Unravels a Metabolic Niche and the Genetic Basis of Trichome‐Mediated Stress Adaptation

Advanced Science, EarlyView.
This study constructed the first spatiotemporal multi‐omics map of peach fruit and discovered a key candidate gene that synergistically regulates trichome development and drought tolerance through the jasmonic acid signaling pathway, providing insights into the coupling mechanism between development and stress resistance.
Zhixin Liu, Ke Cao, Liping Guan, Aizhi Qin, Yong Li, Zihao Zhao, Yinpeng Zhang, Yaping Zhou, Lirong Wang, Xuwu Sun   +9 more
wiley   +1 more source

Large-Scale Gene Disruption Using the UAU1 Cassette [PDF]

, 2009
Candida albicans is a major fungal systemic pathogen in humans. Genetic manipulation of C. albicans is unwieldy. We report here a strategy that is useful and successful for large-scale genetic manipulation of C. albicans genes of interest: use of the UAU1 cassette on a Tn7 transposon. Streamlined yet admittedly flawed disruption techniques, such as the
Clarissa J, Nobile, Aaron P, Mitchell
openaire   +2 more sources

Cis‐ and Trans‐Regulatory Factors Independently Shape Phenotypic Heterogeneity of Retinitis Pigmentosa

Advanced Science, EarlyView.
A zebrafish model carrying an identical human RHO S334X allele reveals two independent genetic layers shaping retinitis pigmentosa (RP) severity: a protective 3‐bp cis‐regulatory insertion that attenuates transgene expression, and a dominant trans‐acting modifier that restores a severe phenotype.
Cong Cui, Kotone Nakagawa, Takumi Tateno, Ayaka Dan, Dexin Meng, Yoshihiro Omori, Soma Tomihara, Suzuri Okamoto, Shigeru Sato, Motokazu Tsujikawa   +9 more
wiley   +1 more source

Belt and braces: Two escape ways to maintain the cassette reservoir of large chromosomal integrons.

PLoS Genetics
Integrons are adaptive devices that capture, stockpile, shuffle and express gene cassettes thereby sampling combinatorial phenotypic diversity. Some integrons called sedentary chromosomal integrons (SCIs) can be massive structures containing hundreds of ...
Egill Richard, Baptiste Darracq, Eloi Littner, Gael A Millot, Valentin Conte, Thomas Cokelaer, Jan Engelstädter, Eduardo P C Rocha, Didier Mazel, Céline Loot   +9 more
doaj   +1 more source

Analysis of antibiotic resistance gene cassettes in a newly identified Salmonella enterica serovar Gallinarum strain in Korea. [PDF]

Mob DNA, 2023
Tran TQ, Park M, Lee JE, Kim SH, Jeong JH, Choy HE.   +5 more
europepmc   +1 more source

UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus Propagation

Advanced Science, EarlyView.
ABSTRACT Flavivirus subgenomic RNAs (sfRNAs) antagonise antiviral defences, yet how sfRNAs are organized and maintained in cells remains poorly understood. Here we identify ubiquitin C‐terminal hydrolase L3 (UCHL3) as a post‐translational regulator of flavivirus sfRNA stability and function.
Oscar Trejo‐Cerro, Anna Beekmayer‐Dhillon, Qi Wen Teo, Lewis Siu, Ming Yuan Li, Kate J. Heesom, Adán Pinto‐Fernández, Sumana Sanyal   +7 more
wiley   +1 more source

Identification and characterization of class 1 integrons among atypical enteropathogenic Escherichia coli isolated from children under 5 years of age

Iranian Journal of Microbiology, 2014
Background and Objectives: The therapeutic options for diseases caused by Escherichia coli are limited. In this study we investigated the presence of virulence factors among Enteropathogenic Escherichia coli (EPEC) strains and their antibiotic resistance
Farzad Shahcheraghi, Fatemeh Rahmati Ghezelgeh, Saman Nobari, Elham Torabi, Seyed-Fazlollah Mousavi, Mohammad Mehdi Aslani, Fereshteh Shahcheraghi   +6 more
doaj