Results 71 to 80 of about 95,867 (295)
Construction of a novel fungal gus expression plasmid, and its evaluation in Aspergillus nidulans : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Genetics at Massey University [PDF]
, 1996 A GUS expression plasmid, pFunGus, was constructed containing a multi-cloning site for the insertion of gene regulatory elements, to be used in fungal reporter gene studies.
McGowan, Tania Louise
core
Translesion synthesis DNA polymerase η exhibits a specific RNA extension activity and a transcription-associated function [PDF]
, 2017 We thank Andres Aguilera for providing the pCYC-LacZ plasmid for the GLRO experiments, and Szilvia Minorits for technical assistance. This work was also supported by grants from the National Research, Development and Innovation Office: GINOP-2.3.2-15 ...
Balint, Eva +5 more
core +3 more sources
Large-Scale Gene Disruption Using the UAU1 Cassette [PDF]
, 2009 Candida albicans is a major fungal systemic pathogen in humans. Genetic manipulation of C. albicans is unwieldy. We report here a strategy that is useful and successful for large-scale genetic manipulation of C. albicans genes of interest: use of the UAU1 cassette on a Tn7 transposon. Streamlined yet admittedly flawed disruption techniques, such as the
Clarissa J, Nobile, Aaron P, Mitchell
openaire +2 more sources
Characterization of the novel In1059 harbouring VIM gene cassette [PDF]
Antimicrobial Resistance & Infection Control, 2017 VIM-type enzyme encodes the most widely acquired metallo-β-lactamases in Gram- negative bacteria. To obtain current epidemiological data for integrons from enterobacteriae in hospital, the study characterizes the genetic structure in In1059 by comparison with In846 integrons harbouring VIM gene and other class 1 integrons including In37, In62, In843 ...
Dongguo Wang +6 more
openaire +3 more sources
Advanced Functional Materials, EarlyView.The nanostructure, size, and function of mRNA‐loaded lipid nanoparticles are evaluated before drying, within polymer microneedles, and after rehydration. The results reveal the polymer and LNP loadings required to recover nanostructure and preserve the delivery performance in dry‐state formulations.
Brendan P. Dyett +19 more
wiley +1 more source
New Microbes and New Infections, 2015 A total of 189 Acinetobacter baumannii isolates were collected in 2011 from a teaching hospital in Chongqing, China. Susceptibility data showed strains carrying integrons were significantly more resistant to all tested antibiotics that strains lacking ...
C. Huang +6 more
doaj +1 more source
Applied Biotechnology to combat the late blight in potato caused by Phytophthora infestans [PDF]
, 2009 Potato is an important crop, grown worldwide. It suffers from many pests and diseases among which late blight, caused by the oomycete Phytophthora infestans, is the worst.
Haverkort, A.J. +3 more
core +2 more sources
Advanced Healthcare Materials, EarlyView.Machine learning–guided engineering of a plectasin‐derived peptide yields DC05, a potent antimycobacterial candidate. Encapsulation into tuftsin‐functionalized mesoporous silica nanoparticles enhances intracellular delivery, stability, and activity against Mycobacterium tuberculosis while maintaining low cytotoxicity and minimal hemolysis. The combined
Christian S. Carnero Canales +12 more
wiley +1 more source
Rad51/Dmc1 paralogs and mediators oppose DNA helicases to limit hybrid DNA formation and promote crossovers during meiotic recombination [PDF]
, 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original ...
Lorenz, Alexander +3 more
core +1 more source
Gene Cassette PCR: Sequence-Independent Recovery of Entire Genes from Environmental DNA [PDF]
Applied and Environmental Microbiology, 2001 ABSTRACT The vast majority of bacteria in the environment have yet to be cultured. Consequently, a major proportion of both genetic diversity within known gene families and an unknown number of novel gene families reside in these uncultured organisms. Isolation of these genes is limited by lack of sequence information.
H W, Stokes +6 more
openaire +2 more sources