Results 71 to 80 of about 2,576,180 (238)

Construction of heterologous gene expression cassettes for the development of recombinant Clostridium beijerinckii

, 2020
Gene-expression cassettes for the construction of recombinant Clostridium beijerinckii were developed as potential tools for metabolic engineering of C. beijerinckii.
박시재
core   +1 more source

A study of clinical strains of Pseudomonas Aeruginosa and the investigation of antibiotic resistance mechanisms in the multidrug resistant strain PA13 [PDF]

, 2008
Thirteen clinical strains of bacteria from two Irish hospitals were identified as Pseudomonas aeruginosa using classical methods, API 20NE and Biolog GN. Their identification was confirmed by 16S rRNA gene sequencing.
Ferguson, Damien
core   +2 more sources

Esterified Cholesterol Conjugates Enable LNP‐Mediated mRNA Delivery to the Blood–Brain Barrier Following Intravenous Administration

Advanced Materials, EarlyView.
A cholesterol‐conjugated lipid library enabled the identification of a ligand‐free LNP platform for efficient mRNA delivery to brain endothelial cells via systemic administration. This platform achieves selective BBB targeting without disrupting barrier integrity, and enables modulation of neuroinflammation and vascular function without requiring trans‐
Zeru Tian, Xu Wang, Alexis Pacheco Benitez, Erick Guerrero, Yaou Duan, Jingwen Wei, Lukas Farbiak, Daniel J. Siegwart   +7 more
wiley   +1 more source

Distribution diversity and expression regulation of class 1 integron promoters in clinical isolates of Morganella morganii

Frontiers in Microbiology
BackgroundMorganella morganii is an emerging nosocomial opportunistic pathogen with increasing multidrug resistance. Antibiotic resistance, driven primarily by the horizontal transfer of resistance genes, has become a global health crisis.
Ye Yang, Hui Zhang, Rongqing Zhao, Xuedan Qiu, Jinglu Ye, Wenjun Lu, Qingcao Li, Guangliang Wu   +7 more
doaj   +1 more source

PCR- and Ligation-Mediated Synthesis of Split-Marker Cassettes with Long Flanking Homology Regions for Gene Disruption in Candida albicans

BioTechniques, 2000
Because Candida albicans is a diploid organism, two consecutive steps of gene disruption are required to generate a gene knock-out. The same marker (URA3) is often used for disruption of both copies of the gene.
Ronald de Hoogt, Walter H.M.L. Luyten, Roland Contreras, Marianne D. De Backer   +3 more
doaj   +1 more source

Modulation of miR‐23b Wnt/β‐catenin Axis Strengthens Endothelial Barrier Properties

Advanced Science, EarlyView.
Early blood‐brain barrier (BBB) disruption contributes to stroke and CNS disease pathology. miR‐23b was identified as a regulator of BBB integrity in brain endothelial cells. Inhibition of miR‐23b enhanced barrier‐associated properties, promoted repair‐related signaling, and reduced BBB leakage in experimental stroke models, supporting further ...
Victor Anthony Martinez, Rose E. Presby, Sara Nakanishi, Pallavi Gaur, Ugur Akcan, Johannes Daugaard Krog, Iben Daugaard Krog, Lipi Das, David S. Johnson, Danny Jamoul, Douglas Jury, Brian Lawson, Jacob Körbelin, Lewis M. Brown, Vilas Menon, Dritan Agalliu, Irene Munk Pedersen   +16 more
wiley   +1 more source

Repository of Antibiotic resistance Cassettes / gene cassettes of unknown function

The cassette repository was compiled from a previous review (Partridge SR, Tsafnat G, Coiera E et al. Gene cassettes and cassette arrays in mobile resistance integrons.

core   +1 more source

Natural transformation with synthetic gene cassettes: new tools for integron research and biotechnology

, 2011
Integrons are genetic elements that can capture and express genes packaged as gene cassettes. Here we report new methods that allow integrons to be studied and manipulated in their native bacterial hosts.
Elissa F. Liew, Nicholas V. Coleman, Alicia M. Gestal   +2 more
core   +1 more source

Characterization of Novel Integrons, In1085 and In1086, and the Surrounding Genes in Plasmids from Enterobacteriaceae, and the Role for attCaadA16 Structural Features during attI1 × attC Integration

Frontiers in Microbiology, 2017
Novel class 1 integrons In1085 and In1086, containing the class D β-lactamase -encoding gene blaOXA, were identified in clinical enterobacterial strains.
Dongguo Wang, Wei Hou, Jiayu Chen, Linjun Yang, Zhihui Liu, Zhe Yin, Jiao Feng, Dongsheng Zhou   +7 more
doaj   +1 more source

Attomolar Detection of HIV‐1 With Label‐Free RCA‐rCRISPR on Smartphone

Advanced Science, EarlyView.
A new diagnostic strategy integrating rolling circle amplification (RCA) with a plasmid reporter–based ratiometric CRISPR (rCRISPR) system was developed to detect unfragmented HIV genomes with single‐digit attomolar sensitivity. The method quantifies targets using the plasmid relaxation ratio and employs a smartphone‐based mini‐gel device to enable ...
Noor Mohammad, Anastasiia Steksova, Yuyang Tang, Leyao Huang, Alireza Velayati, Shengwei Zhang, Aditi Dey Poonam, Sina Jamalzadegan, Matthew Breen, Hongjie Chen, Mahsa Bagi, Guochun Jiang, Qingshan Wei   +12 more
wiley   +1 more source