Results 71 to 80 of about 54,378 (282)

A novel cassette method for probe evaluation in the designed biochips. [PDF]

PLoS ONE, 2014
A critical step in biochip design is the selection of probes with identical hybridisation characteristics. In this article we describe a novel method for evaluating DNA hybridisation probes, allowing the fine-tuning of biochips, that uses cassettes with ...
Vitaly Zinkevich, Nelly Sapojnikova, Julian Mitchell, Tamar Kartvelishvili, Nino Asatiani, Samia Alkhalil, Irina Bogdarina, Abdulmohsen A Al-Humam   +7 more
doaj   +1 more source

Identification of promoter activity in gene-less cassettes from Vibrionaceae superintegrons

Integrons are genetic platforms that acquire new genes encoded in integron cassettes (ICs), building arrays of adaptive functions. ICs generally encode promoterless genes, whose expression relies on the platform-associated Pc promoter, with the cassette ...
Vergara, Ester, Blanco Torres, Paula, Ortega, Alba Cristina, Hipólito, Alberto, Escudero, José Antonio, Toribio-Celestino, Laura, Escudero García-Calderón, José Antonio, San Millán, Álvaro, Blanco, Paula, García-Pastor, Lucía, Trigo da Roza, Filipa, García Pastor, Lucía   +11 more
core   +1 more source

Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes

, 2002
This study was aimed at determining whether gene cassettes (promoter-coding sequence-terminator) can be efficiently used in microprojectile acceleration-mediated co-transformation of rice in the place of whole plasmids, and to what extent their use ...
Meynard, Donaldo, Guiderdoni, Emmanuel, Legavre, Thierry, Labeyrie, Axel, Breitler, Jean-Christophe   +4 more
core   +1 more source

Functional Blood‐Brain Barrier Crossing by Biomimetic M13 Phage Vectors for Targeted Neuronal Delivery

Advanced Healthcare Materials, EarlyView.
This study investigates the M13 bacteriophage as a biomimetic nanovector capable of crossing in vitro models of the blood–brain barrier. By exploiting peculiar transcellular pathways, M13 avoids lysosomal degradation and preserves its structural integrity and functionality.
Silvia Vercellino, Lucia Pappagallo, Francesca De Chirico, Luca Boselli, Hazem Ahmed, Luca Spagnoletti, Giovanni Pittaluga, Paolo Emidio Costantini, Matteo Calvaresi, Pier Paolo Pompa, Alberto Danielli, Fabio Benfenati, Valentina Castagnola   +12 more
wiley   +1 more source

Exploring the role of mobile genetic elements in shaping plant–bacterial interactions for sustainable agriculture and ecosystem health

Plants, People, Planet
Societal Impact Statement Plants and bacteria interact in complex ways that are crucial to the health and productivity of native vegetation and croplands. While the range of characterised plant‐beneficial bacterial traits continues to grow, key questions
Vaheesan Rajabal, Timothy M. Ghaly, Eleonora Egidi, Mingjing Ke, Anahit Penesyan, Qin Qi, Michael R. Gillings, Sasha G. Tetu   +7 more
doaj   +1 more source

Affinity‐Tuned Albumin Hitchhiking Extends the Bioorthogonal Capture Window in Pretargeting Radiotheranostics

Advanced Healthcare Materials, EarlyView.
Pharmacokinetic engineering of clickable radioligands was achieved via albumin hitchhiking approach within a pretargeted radiotheranostics delivery platform. This approach reduced rapid renal elimination, expanded bioorthogonal capture window, boosted tumor uptake by ∼3.5‐fold, and improved the tumor‐to‐liver ratio by ∼6‐fold.
Xie He, Nicholas L. Fletcher, Gayathri R. Ediriweera, Yifei Zhu, James Humphries, Christopher B. Howard, Jiazheng Wang, Craig A. Bell, Kristian Kempe, Kristofer J. Thurecht   +9 more
wiley   +1 more source

Construction of heterologous gene expression cassettes for the development of recombinant Clostridium beijerinckii

, 2020
Gene-expression cassettes for the construction of recombinant Clostridium beijerinckii were developed as potential tools for metabolic engineering of C. beijerinckii.
박시재
core   +1 more source

Targeting UXS1‐Dependent Glucuronate Detoxification Potentiates Metformin's Anti‐Tumor Efficacy in Lung Adenocarcinoma

Advanced Science, EarlyView.
This study reveals that metformin promotes glucuronic acid metabolism in lung adenocarcinoma by activating UGDH S476 phosphorylation and enhancing the conversion of UDPG to UDPGA based on metabolomics analysis. Through compound virtual screening, it is found that plantainoside targeting UGDH downstream UXS1 leads to UDPGA toxicity accumulation ...
Qihai Sui, Zhencong Chen, Guangyao Shan, Zhengyang Hu, Xing Jin, Jiaqi Liang, Yanjun Yi, Jiacheng Yin, Haochun Shi, Xifei Jiang, Junjie Xi, Zongwu Lin, Cheng Zhan, Fenghao Sun, Wei Jiang   +14 more
wiley   +1 more source

Marburg Virus Glycoprotein Is a Remarkable Virulent Factor Linked to Hemorrhagic Pathology: Evidence from Multimodal Experimental Systems

Advanced Science, EarlyView.
By integrating data from in vitro, ex vivo, and in vivo models, our research identifies the MARV glycoprotein as a remarkable hemorrhagic factor, filling a major gap in this important field. It also provides practical experimental tools for the basic research on viral pathogenesis and applied research aimed at antiviral intervention for hemorrhagic ...
Ting Yao, Hang Liu, Yanfeng Yao, Wei Deng, Jiawen Sun, Zhenyu Kang, Ashaq Ali, Chao Shan, Zhiming Yuan, Fei Deng, Hualin Wang, Yun‐Jia Ning   +11 more
wiley   +1 more source

Effect of attC structure on cassette excision by integron integrases

Mobile DNA, 2011
Background Integrons are genetic elements able to integrate and disseminate genes as cassettes by a site-specific recombination mechanism. These elements contain a gene coding for an integrase that carries out recombination by interacting with two ...
Larouche André, Roy Paul H
doaj   +1 more source