Results 61 to 70 of about 54,378 (282)
Gene Cassettes Potentially Encoding Fosfomycin Resistance Determinants [PDF]
Antimicrobial Agents and Chemotherapy, 2005 Gene cassettes are small mobile genetic elements that are found in arrays of one or more cassettes within an integron (5, 9). In addition to a single open reading frame, or sometimes two, each cassette contains a unique recombination site that belongs to a family of sites known as 59-be (59-base elements).
Sally R, Partridge, Ruth M, Hall
openaire +2 more sources
FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source
Iranian Journal of Microbiology, 2018 Background and Objectives: Integrons play a major role in the transmission and accumulation of resistance factors in multidrug resistant bacteria. This study was aimed to evaluate the gene cassettes of class I integron and antimicrobial resistance in ...
Roya Chegene Lorestani +3 more
doaj
Frontiers in Microbiology, 2015 Integrons are genetic units characterized by ability to capture and incorporate gene cassettes that can contribute to emergence and transfer of antibiotic resistance between bacteria.
Mai eMalek +5 more
doaj +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
BMC Infectious Diseases, 2021 Background Productions of metallo-β-lactamases enzymes are the most common mechanism of antibiotic resistance to all beta-lactam classes (except monobactams) in Acinetobacter baumannii.
Mahnaz Nikibakhsh +4 more
doaj +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
New Microbes and New Infections, 2015 A total of 189 Acinetobacter baumannii isolates were collected in 2011 from a teaching hospital in Chongqing, China. Susceptibility data showed strains carrying integrons were significantly more resistant to all tested antibiotics that strains lacking ...
C. Huang +6 more
doaj +1 more source
Advanced Functional Materials, EarlyView.The nanostructure, size, and function of mRNA‐loaded lipid nanoparticles are evaluated before drying, within polymer microneedles, and after rehydration. The results reveal the polymer and LNP loadings required to recover nanostructure and preserve the delivery performance in dry‐state formulations.
Brendan P. Dyett +19 more
wiley +1 more source
, 2005 The ease of construction of multiple mutant strains in Schizosaccharomyces pombe is limited by the number of available genetic markers. We describe here three new cassettes for PCR-mediated gene disruption that can be used in combination with commonly ...
Jean Vandenhaute (16066397) +4 more
core +2 more sources