Results 41 to 50 of about 3,120,234 (211)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Gene cassettes of class I integron-associated with antimicrobial resistance in isolates of Citrobacter spp. with multidrug resistance

Iranian Journal of Microbiology, 2018
Background and Objectives: Integrons play a major role in the transmission and accumulation of resistance factors in multidrug resistant bacteria. This study was aimed to evaluate the gene cassettes of class I integron and antimicrobial resistance in ...
Roya Chegene Lorestani, Alisha Akya, Azam Elahi, Yazdan Hamzavi   +3 more
doaj  

Occurrence Of Class 1 And 2 Integrons In Enterobacteriaceae Collected From Zagazig University Hospitals, Egypt

Frontiers in Microbiology, 2015
Integrons are genetic units characterized by ability to capture and incorporate gene cassettes that can contribute to emergence and transfer of antibiotic resistance between bacteria.
Mai eMalek, Fatma Abdelaziz Amer, Ayman eAllam, Rehab eEl-Sokkary, Tarek eGheith, Mohamed eArafa   +5 more
doaj   +1 more source

Natural transformation with synthetic gene cassettes: new tools for integron research and biotechnology

, 2011
Integrons are genetic elements that can capture and express genes packaged as gene cassettes. Here we report new methods that allow integrons to be studied and manipulated in their native bacterial hosts.
Elissa F. Liew, Nicholas V. Coleman, Alicia M. Gestal   +2 more
core   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Molecular study of metallo-β-lactamases and integrons in Acinetobacter baumannii isolates from burn patients

BMC Infectious Diseases, 2021
Background Productions of metallo-β-lactamases enzymes are the most common mechanism of antibiotic resistance to all beta-lactam classes (except monobactams) in Acinetobacter baumannii.
Mahnaz Nikibakhsh, Farzaneh Firoozeh, Farzad Badmasti, Kourosh Kabir, Mohammad Zibaei   +4 more
doaj   +1 more source

Schematic diagram of resistance gene cassettes located in the class 1 integron from Stenotrophomonas maltophilia isolates.

, 2016
Schematic diagram of resistance gene cassettes located in the class 1 integron from Stenotrophomonas maltophilia isolates.
Jia-Bin Li (2179006), Li-Ping Gao (609913), Li-Fen Hu (2839187), Ying Ye (72583), Guo-Sheng Chen (2839190), Qin-Xiang Kong (2839184), Xi Chen (35903)   +6 more
core   +1 more source

Molecular epidemiological analysis of integron gene cassettes and tetA/tetB/tetD gene associations in Escherichia coli strains producing extended-spectrum beta-lactamase (ESBL) in urine cultures [PDF]

, 2021
Background. Epidemiological studies of tetracycline (TE) resistance genes and integron gene cassettes, particularly in urine samples, are limited in Turkey. Objectives.
Kalcan, Sema Koçyiğit, Çiçek, Ayşegül Çopur, Gündoğdu, Deniz Zehra Ulusan, Şemen, Vicdan, Köse, Fatma Tufan, Ejder, Nebahat Aydoğan, Özgümüş, Osman Birol, Deniz Gündoğdu, Ayşegül ÇİÇEK, Vicdan ŞEMEN, Fatma KÖSE, Sema Kalcan, Osman ÖZGÜMÜŞ, Nebahat AYDOĞAN EJDER   +13 more
core   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

Trade-off between canonical and unusual recombination sites promotes diversity and stability of gene cassette arrays of mobile integrons

Scientific Reports
Mobile integrons are the most efficient mechanism of Gram-negative bacteria to resist antimicrobial changing pressures in the nosocomial niche. Integron’s integrases mediate site-specific recombination of distinct DNA structures such as attI, attC and ...
Adrián Gonzales Machuca, María Carolina Molina, Verónica Elizabeth Álvarez, Eduardo José Carpio Díaz, María Paula Quiroga, Daniela Centrón   +5 more
doaj   +1 more source